PMID- 37249337
OWN - NLM
STAT- MEDLINE
DCOM- 20230621
LR  - 20240102
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Print)
IS  - 1672-9145 (Linking)
VI  - 55
IP  - 5
DP  - 2023 May 29
TI  - NQO1/CPT1A promotes the progression of pancreatic adenocarcinoma via fatty acid 
      oxidation.
PG  - 758-768
LID - 10.3724/abbs.2023066 [doi]
AB  - NQO1, a cytosolic enzyme, is closely related to the progression of cancers and 
      poor outcome of cancer patients. However, the molecular biological mechanism of 
      NQO1 tumorigenicity in pancreatic adenocarcinoma (PAAD) has not been clearly 
      understood. In this study, we demonstrate the molecular mechanism of NQO1 in PAAD 
      proliferation, metastasis and fatty acid oxidation (FAO). Multiple databases and 
      western blot analysis show that NQO1 is overexpressed in PAAD and associated with 
      lymph node metastasis and shorter survival. Furthermore, in vitro and in vivo 
      experiments reveal that overexpression of NQO1 improves tumor growth, metastasis 
      and FAO in PAAD. Mechanistically, NQO1 is able to bind to carnitine 
      palmitoyltransferase 1A (CPT1A), a key enzyme controlling FAO. Therefore, Co-IP 
      and a series of rescue experiments demonstrate that NQO1 promotes PAAD 
      progression via CPT1A-mediated FAO. Our findings identify CPT1A-dependent FAO as 
      an essential metabolic pathway for NQO1 to promote the PAAD process. Targeting 
      the NQO1/CPT1A/FAO axis in PAAD to attenuate proliferation and dissemination is a 
      potential approach to promote a better antitumour effect and improve patient 
      outcomes.
FAU - Xu, Ran
AU  - Xu R
AD  - Inner Mongolia Minzu University, Tongliao 028000, China.
AD  - Key Laboratory of Pathobiology of High Frequency Oncology in Ethnic Minority 
      Areas (Yanbian University), State Ethnic Affairs Commission, Yanji 133000, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Key Laboratory of Pathobiology of High Frequency Oncology in Ethnic Minority 
      Areas (Yanbian University), State Ethnic Affairs Commission, Yanji 133000, China.
FAU - Ma, Liang
AU  - Ma L
AD  - Department of General Surgery, Siping Central People Hospital, Siping 136000, 
      China.
FAU - Sun, Yao
AU  - Sun Y
AD  - Department of General Surgery, Siping Central People Hospital, Siping 136000, 
      China.
FAU - Liu, Haifeng
AU  - Liu H
AD  - Department of General Surgery, Siping Central People Hospital, Siping 136000, 
      China.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of General Surgery, Siping Central People Hospital, Siping 136000, 
      China.
AD  - Key Laboratory of Pathobiology of High Frequency Oncology in Ethnic Minority 
      Areas (Yanbian University), State Ethnic Affairs Commission, Yanji 133000, China.
FAU - Jin, Tiefeng
AU  - Jin T
AD  - Key Laboratory of Pathobiology of High Frequency Oncology in Ethnic Minority 
      Areas (Yanbian University), State Ethnic Affairs Commission, Yanji 133000, China.
FAU - Yang, Dawei
AU  - Yang D
AD  - Department of General Surgery, Siping Central People Hospital, Siping 136000, 
      China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
RN  - 0 (Fatty Acids)
RN  - EC 1.6.5.2 (NQO1 protein, human)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
SB  - IM
MH  - Humans
MH  - *Adenocarcinoma/genetics
MH  - Cell Line, Tumor
MH  - Carnitine O-Palmitoyltransferase/genetics/metabolism
MH  - *Pancreatic Neoplasms/genetics
MH  - Fatty Acids/metabolism
MH  - NAD(P)H Dehydrogenase (Quinone)/genetics
PMC - PMC10281888
OTO - NOTNLM
OT  - CPT1A
OT  - NQO1
OT  - PAAD
OT  - fatty acid oxidation
OT  - metastasis
COIS- The authors declare that they have no conflict of interest.
EDAT- 2023/05/30 13:07
MHDA- 2023/06/21 06:42
PMCR- 2023/05/29
CRDT- 2023/05/30 09:12
PHST- 2023/06/21 06:42 [medline]
PHST- 2023/05/30 13:07 [pubmed]
PHST- 2023/05/30 09:12 [entrez]
PHST- 2023/05/29 00:00 [pmc-release]
AID - 10.3724/abbs.2023066 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2023 May 29;55(5):758-768. doi: 
      10.3724/abbs.2023066.