PMID- 37249774 OWN - NLM STAT- MEDLINE DCOM- 20230629 LR - 20230629 IS - 1614-7499 (Electronic) IS - 0944-1344 (Linking) VI - 30 IP - 31 DP - 2023 Jul TI - Construction of a novel MPT-driven necrosis-related lncRNAs signature for prognosis prediction in laryngeal squamous cell carcinoma. PG - 77210-77225 LID - 10.1007/s11356-023-26996-1 [doi] AB - Mitochondrial permeability transition (MPT)-driven necrosis, a type of programmed cell death, has recently gained much attention in a variety of tumor types. Few studies have been performed to explore the role of MPT-driven necrosis-related lncRNAs (MPTDNRlncRNAs) in laryngeal squamous cell carcinoma (LSCC). The purpose of our study is to screen MPTDNRlncRNAs with prognostic value and to explore their potential roles in LSCC. The RNA-sequencing data and the corresponding clinical data of LSCC patients were obtained from the TCGA database, and those MPT-driven necrosis-related genes were extracted from the Gene Set Enrichment Analysis (GSEA) database. We identified MPTDNRlncRNAs differentially expressed in LSCC. Also, we gained MPT-driven necrosis-related prognostic lncRNAs by univariate cox regression analysis. A novel MPTDNRlncRNAs signature was constructed by LASSO-COX. The accuracy and utility of the MPTDNRlncRNAs signature were evaluated via a variety of statistical methods. Multiple bioinformatics tools were used to explore the underlying difference in biological functions and signaling pathways between the different risk groups. The expressions levels of MPTDNRlncRNAs were analyzed using RT-qPCR in LSCC cell line. Finally, we identified A 5 MPTDNRlncRNAs signature in LSCC. Our prognostic model demonstrated an efficient ability to predict outcomes. The proportion difference of immune cells in the subgroups were significant, such as M0 macrophage and T follicular helper cells. The in vitro experiments suggested that our MPTDNRlncRNAs were significantly different. This 5 MPTDNRlncRNAs signature is a prognostic biomarker for LSCC. More importantly, the novel biologic prognostic model can be utilized for personalized immunotherapy in LSCC patients. CI - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Liu, Jian AU - Liu J AD - Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Affilated to Fudan University, Shanghai, 201700, People's Republic of China. FAU - Zhang, Min AU - Zhang M AD - Xiangya Hospital, Central South University, Changsha, Hunan, 41000, People's Republic of China. FAU - Sun, Qing AU - Sun Q AD - Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Affilated to Fudan University, Shanghai, 201700, People's Republic of China. FAU - Qin, Xuemei AU - Qin X AD - Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Affilated to Fudan University, Shanghai, 201700, People's Republic of China. FAU - Gao, Tianle AU - Gao T AD - Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Affilated to Fudan University, Shanghai, 201700, People's Republic of China. FAU - Xu, Yinwei AU - Xu Y AD - Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Affilated to Fudan University, Shanghai, 201700, People's Republic of China. FAU - Han, Shuhui AU - Han S AD - Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Affilated to Fudan University, Shanghai, 201700, People's Republic of China. FAU - Zhang, Yujie AU - Zhang Y AD - Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Affilated to Fudan University, Shanghai, 201700, People's Republic of China. FAU - Guo, Zhiqiang AU - Guo Z AD - Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Affilated to Fudan University, Shanghai, 201700, People's Republic of China. gzqhblove@126.com. LA - eng GR - QYT2021-02/the Hospital-level Project of QingPu Branch of Zhongshan Hospital Affiliated to Fudan University/ GR - WD2019-13/the Discipline Leader Training Program of Health Commission/ GR - Qingpu District/the Discipline Leader Training Program of Health Commission/ GR - Shanghai/the Discipline Leader Training Program of Health Commission/ GR - PR China/the Discipline Leader Training Program of Health Commission/ GR - W2021-03/the Project of Health Commission/ GR - Qingpu District/the Project of Health Commission/ GR - Shanghai/the Project of Health Commission/ GR - PR China/the Project of Health Commission/ PT - Journal Article DEP - 20230530 PL - Germany TA - Environ Sci Pollut Res Int JT - Environmental science and pollution research international JID - 9441769 RN - 0 (RNA, Long Noncoding) SB - IM MH - Humans MH - *RNA, Long Noncoding/genetics MH - Mitochondrial Transmembrane Permeability-Driven Necrosis MH - Squamous Cell Carcinoma of Head and Neck/genetics MH - Necrosis MH - *Head and Neck Neoplasms OTO - NOTNLM OT - Immune microenvironment OT - Laryngeal squamous cell carcinoma OT - LncRNAs OT - MPT-driven necrosis OT - Prognostic models EDAT- 2023/05/30 13:07 MHDA- 2023/06/29 06:43 CRDT- 2023/05/30 11:12 PHST- 2023/01/12 00:00 [received] PHST- 2023/04/09 00:00 [accepted] PHST- 2023/06/29 06:43 [medline] PHST- 2023/05/30 13:07 [pubmed] PHST- 2023/05/30 11:12 [entrez] AID - 10.1007/s11356-023-26996-1 [pii] AID - 10.1007/s11356-023-26996-1 [doi] PST - ppublish SO - Environ Sci Pollut Res Int. 2023 Jul;30(31):77210-77225. doi: 10.1007/s11356-023-26996-1. Epub 2023 May 30.