PMID- 37250396
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231005
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 17
DP  - 2023
TI  - High fidelity sensory-evoked responses in neocortex after intravenous injection 
      of genetically encoded calcium sensors.
PG  - 1181828
LID - 10.3389/fnins.2023.1181828 [doi]
LID - 1181828
AB  - Two-photon imaging of genetically-encoded calcium indicators (GECIs) has 
      traditionally relied on intracranial injections of adeno-associated virus (AAV) 
      or transgenic animals to achieve expression. Intracranial injections require an 
      invasive surgery and result in a relatively small volume of tissue labeling. 
      Transgenic animals, although they can have brain-wide GECI expression, often 
      express GECIs in only a small subset of neurons, may have abnormal behavioral 
      phenotypes, and are currently limited to older generations of GECIs. Inspired by 
      recent developments in the synthesis of AAVs that readily cross the blood brain 
      barrier, we tested whether an alternative strategy of intravenously injecting 
      AAV-PHP.eB is suitable for two-photon calcium imaging of neurons over many months 
      after injection. We injected C57BL/6 J mice with AAV-PHP.eB-Synapsin-jGCaMP7s via 
      the retro-orbital sinus. After allowing 5 to 34 weeks for expression, we 
      performed conventional and widefield two-photon imaging of layers 2/3, 4 and 5 of 
      the primary visual cortex. We found reproducible trial-by-trial neural responses 
      and tuning properties consistent with known feature selectivity in the visual 
      cortex. Thus, intravenous injection of AAV-PHP.eB does not interfere with the 
      normal processing in neural circuits. In vivo and histological images show no 
      nuclear expression of jGCaMP7s for at least 34 weeks post-injection.
CI  - Copyright (c) 2023 Leikvoll and Kara.
FAU - Leikvoll, Austin
AU  - Leikvoll A
AD  - Department of Neuroscience, University of Minnesota, Minneapolis, MN, United 
      States.
FAU - Kara, Prakash
AU  - Kara P
AD  - Department of Neuroscience, University of Minnesota, Minneapolis, MN, United 
      States.
LA  - eng
GR  - T32 GM008244/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20230512
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
UOF - bioRxiv. 2023 Mar 10;:. PMID: 36945523
PMC - PMC10213453
OTO - NOTNLM
OT  - AAV-PHP GCaMP7
OT  - blood brain barrier
OT  - brain-wide calcium imaging
OT  - long-term imaging
OT  - trial-by-trial variability
OT  - two-photon microscopy
OT  - visual cortex (V1)
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/05/30 13:07
MHDA- 2023/05/30 13:08
PMCR- 2023/01/01
CRDT- 2023/05/30 11:23
PHST- 2023/03/07 00:00 [received]
PHST- 2023/04/12 00:00 [accepted]
PHST- 2023/05/30 13:08 [medline]
PHST- 2023/05/30 13:07 [pubmed]
PHST- 2023/05/30 11:23 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2023.1181828 [doi]
PST - epublish
SO  - Front Neurosci. 2023 May 12;17:1181828. doi: 10.3389/fnins.2023.1181828. 
      eCollection 2023.