PMID- 37255101
OWN - NLM
STAT- MEDLINE
DCOM- 20230831
LR  - 20230921
IS  - 2051-6355 (Electronic)
IS  - 2051-6347 (Linking)
VI  - 10
IP  - 9
DP  - 2023 Aug 29
TI  - Ultrasound-triggered in situ gelation with ROS-controlled drug release for 
      cartilage repair.
PG  - 3507-3522
LID - 10.1039/d3mh00042g [doi]
AB  - Cartilage defects are usually caused by acute trauma and chronic degeneration. 
      However, it is still a great challenge to improve the repair of articular 
      cartilage defects due to the limited self-regeneration capacity of such defects. 
      Herein, a novel ROS-responsive in situ nanocomposite hydrogel loaded with 
      kartogenin (KGN) and bone marrow-derived stem cells (BMSCs) was designed and 
      constructed via the enzymatic reaction of fibrinogen and thrombin. Meanwhile, a 
      ROS-responsive thioketal (TK)-based liposome was synthesized to load the 
      chondrogenesis-inducing factor KGN, the bioenzyme thrombin and an 
      ultrasound-sensitive agent PpIX. Under ultrasound stimulation, the TK-based 
      liposome was destroyed, followed by in situ gelation of fibrinogen and thrombin. 
      Moreover, sustained release of KGN was realized by regulating the ultrasound 
      conditions. Importantly, ROS generation and KGN release within the 
      microenvironment of the in situ fibrin hydrogel significantly promoted 
      chondrogenic differentiation of BMSCs via the Smad5/mTOR signalling pathway and 
      effectively improved cartilage regeneration in a rat articular cartilage defect 
      model. Overall, the novel in situ nanocomposite hydrogel with ROS-controlled drug 
      release has great potential for efficient cartilage repair.
FAU - Wu, Shunli
AU  - Wu S
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
AD  - School of Medicine, Shanghai University, Shanghai 200444, China.
AD  - School of Environmental and Chemical Engineering, Shanghai University, Shanghai 
      200444, China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
FAU - Wang, Sicheng
AU  - Wang S
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - School of Medicine, Shanghai University, Shanghai 200444, China.
AD  - Department of Orthopedics, Shanghai Zhongye Hospital, Shanghai, China.
FAU - Sun, Jinru
AU  - Sun J
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
AD  - School of Medicine, Shanghai University, Shanghai 200444, China.
FAU - Hu, Yan
AU  - Hu Y
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
AD  - Shaoxing Institute of Technology at Shanghai University, Shaoxing, 312000, China.
FAU - Liu, Han
AU  - Liu H
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
FAU - Liu, Jinlong
AU  - Liu J
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
FAU - Chen, Xiao
AU  - Chen X
AD  - Department of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Medical 
      University, Shanghai, China.
FAU - Zhou, Fengjin
AU  - Zhou F
AD  - Department of Orthopaedics, Honghui Hospital, Xi'an Jiao Tong University, Xi'an 
      710000, China. dr.zhoufj@163.com.
FAU - Bai, Long
AU  - Bai L
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
FAU - Wang, Xiuhui
AU  - Wang X
AUID- ORCID: 0000-0002-6031-0380
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
FAU - Su, Jiacan
AU  - Su J
AUID- ORCID: 0000-0001-7080-263X
AD  - Institute of Translational Medicine, Shanghai University, Shanghai 200444, China. 
      bailong@shu.edu.cn.
AD  - Organoid Research Center, Shanghai University, Shanghai, 200444, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230829
PL  - England
TA  - Mater Horiz
JT  - Materials horizons
JID - 101623537
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Liposomes)
RN  - 0 (Nanogels)
RN  - EC 3.4.21.5 (Thrombin)
RN  - 0 (Hydrogels)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Reactive Oxygen Species/metabolism/pharmacology
MH  - Drug Liberation
MH  - Liposomes/metabolism/pharmacology
MH  - Nanogels
MH  - Thrombin/metabolism/pharmacology
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *Cartilage, Articular/diagnostic imaging/injuries/metabolism
MH  - Hydrogels
EDAT- 2023/05/31 13:12
MHDA- 2023/08/31 06:41
CRDT- 2023/05/31 07:53
PHST- 2023/08/31 06:41 [medline]
PHST- 2023/05/31 13:12 [pubmed]
PHST- 2023/05/31 07:53 [entrez]
AID - 10.1039/d3mh00042g [doi]
PST - epublish
SO  - Mater Horiz. 2023 Aug 29;10(9):3507-3522. doi: 10.1039/d3mh00042g.