PMID- 37256923
OWN - NLM
STAT- MEDLINE
DCOM- 20230613
LR  - 20230615
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 9
IP  - 6
DP  - 2023 Jun 12
TI  - Bilayer Silk Fibroin/Sodium Alginate Scaffold Delivered hUC-MSCs to Enhance Skin 
      Scarless Healing and Hair Follicle Regeneration with the IRE1/XBP1 Pathway 
      Inhibition.
PG  - 3476-3487
LID - 10.1021/acsbiomaterials.3c00059 [doi]
AB  - Efficient local delivery of mesenchymal stem cells (MSCs) is a decisive factor 
      for their application in regeneration processes. Here, we prepared a biomimetic 
      bilayer silk fibroin/sodium alginate (SF/SA) scaffold to deliver human umbilical 
      mesenchymal stem cells (hUC-MSCs) for wound healing. An SA membrane was prepared 
      by the casting method on the upper layer of the scaffold to simulate the dense 
      epidermal structure. On the lower layer, porous materials simulating the loose 
      structure of the dermis were formed by the freeze-drying method. In vitro, the 
      scaffold was proven to have a high-density pore structure, good swelling 
      property, and suitable degradation rate. The hUC-MSCs could survive on the 
      scaffold for up to 14 days and maintain cell stemness for at least 7 days. In 
      vivo, SF/SA scaffolds loaded with hUC-MSCs (M-SF/SA) were applied to 
      full-thickness defect wounds and compared with the local injection of hUC-MSCs. 
      The M-SF/SA group showed excellent therapeutic efficacy, characterized by 
      induction of macrophage polarization, regulation of TGF-beta expression and collagen 
      components, and enhancement of vascular regeneration, thereby preventing scar 
      formation and promoting hair follicle regeneration. Furthermore, the expression 
      of endoplasmic reticulum stress markers IRE1, XBP1, and CHOP was inhibited 
      significantly in M-SF/SA treatment. In conclusion, the bilayer SF/SA scaffold is 
      an ideal delivery platform for hUC-MSCs, and the M-SF/SA system could locally 
      promote scarless skin healing and hair follicle regeneration by alleviating the 
      IRE1/XBP1 signal pathway.
FAU - Dou, Zhaona
AU  - Dou Z
AUID- ORCID: 0000-0003-2862-7385
AD  - State Key Laboratory of Advanced Technology for Materials Synthesis and 
      Processing, Biomedical Materials and Engineering Research Center of Hubei 
      Province, Wuhan University of Technology, Wuhan, Hubei 430070, China.
FAU - Qiu, Tong
AU  - Qiu T
AUID- ORCID: 0000-0001-6314-0885
AD  - State Key Laboratory of Advanced Technology for Materials Synthesis and 
      Processing, Biomedical Materials and Engineering Research Center of Hubei 
      Province, Wuhan University of Technology, Wuhan, Hubei 430070, China.
FAU - Ren, Yimeng
AU  - Ren Y
AD  - School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of 
      Technology, Wuhan, Hubei 430070, China.
FAU - Wang, Xinyu
AU  - Wang X
AD  - State Key Laboratory of Advanced Technology for Materials Synthesis and 
      Processing, Biomedical Materials and Engineering Research Center of Hubei 
      Province, Wuhan University of Technology, Wuhan, Hubei 430070, China.
FAU - Wen, Quan
AU  - Wen Q
AD  - Yinfeng Academy of Life Science, Yichang Laboratory of Hematopoietic Stem Cells, 
      1109 Gangxing Sanlu, Jinan, Shandong Province 250100, China.
FAU - Shen, Ying
AU  - Shen Y
AD  - State Key Laboratory of Advanced Technology for Materials Synthesis and 
      Processing, Biomedical Materials and Engineering Research Center of Hubei 
      Province, Wuhan University of Technology, Wuhan, Hubei 430070, China.
FAU - Wu, Lin
AU  - Wu L
AD  - State Key Laboratory of Advanced Technology for Materials Synthesis and 
      Processing, Biomedical Materials and Engineering Research Center of Hubei 
      Province, Wuhan University of Technology, Wuhan, Hubei 430070, China.
FAU - Han, Lei
AU  - Han L
AD  - State Key Laboratory of Advanced Technology for Materials Synthesis and 
      Processing, Biomedical Materials and Engineering Research Center of Hubei 
      Province, Wuhan University of Technology, Wuhan, Hubei 430070, China.
FAU - Jiang, Tao
AU  - Jiang T
AD  - School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of 
      Technology, Wuhan, Hubei 430070, China.
FAU - Xia, Xinke
AU  - Xia X
AD  - Institute of Life Science, Yinfeng Biological Group, High-tech Zone, Jinan, 
      Shandong Province 250100, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230531
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
RN  - 9007-76-5 (Fibroins)
RN  - 0 (Alginates)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - 0 (XBP1 protein, human)
RN  - 0 (X-Box Binding Protein 1)
SB  - IM
MH  - Humans
MH  - *Fibroins/pharmacology
MH  - Hair Follicle
MH  - Alginates/pharmacology/chemistry
MH  - Wound Healing
MH  - *Mesenchymal Stem Cells/physiology
MH  - Protein Serine-Threonine Kinases
MH  - X-Box Binding Protein 1/genetics
OTO - NOTNLM
OT  - bilayer silk fibroin/sodium alginate
OT  - hair follicle regeneration
OT  - human umbilical mesenchymal stem cells
OT  - scarless healing
OT  - the IRE1/XBP1 pathway
EDAT- 2023/05/31 19:16
MHDA- 2023/06/13 06:42
CRDT- 2023/05/31 13:52
PHST- 2023/06/13 06:42 [medline]
PHST- 2023/05/31 19:16 [pubmed]
PHST- 2023/05/31 13:52 [entrez]
AID - 10.1021/acsbiomaterials.3c00059 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2023 Jun 12;9(6):3476-3487. doi: 
      10.1021/acsbiomaterials.3c00059. Epub 2023 May 31.