PMID- 37258260
OWN - NLM
STAT- MEDLINE
DCOM- 20230602
LR  - 20230604
IS  - 2982-6799 (Electronic)
IS  - 2982-5164 (Print)
IS  - 2982-5164 (Linking)
VI  - 61
IP  - 2
DP  - 2023 May
TI  - Expression of cytokines and co-stimulatory molecules in the Toxoplasma 
      gondii-infected dendritic cells of C57BL/6 and BALB/c mice.
PG  - 138-146
LID - 10.3347/PHD.22150 [doi]
AB  - Toxoplasma gondii is an intracellular protozoan parasite which can infect most 
      warm-blooded animals and humans. Among the different mouse models, C57BL/6 mice 
      are more susceptible to T. gondii infection compared to BALB/c mice, and this 
      increased susceptibility has been attributed to various factors, including T-cell 
      responses. Dendritic cells (DCs) are the most prominent type of 
      antigen-presenting cells and regulate the host immune response, including the 
      response of T-cells. However, differences in the DC responses of these mouse 
      strains to T. gondii infection have yet to be characterized. In this study, we 
      cultured bone marrow-derived DCs (BMDCs) from BALB/c and C57BL/6 mice. These 
      cells were infected with T. gondii. The activation of the BMDCs was assessed 
      based on the expression of cell surface markers and cytokines. In the BMDCs of 
      both mouse strains, we detected significant increases in the expression of cell 
      surface T-cell co-stimulatory molecules (major histocompatibility complex (MHC) 
      II, CD40, CD80, and CD86) and cytokines (tumor necrosis factor (TNF)-alpha, 
      interferon (IFN)-gamma, interleukin (IL)-12p40, IL-1beta, and IL-10) from 3 h post-T. 
      gondii infection. The expression of MHC II, CD40, CD80, CD86, IFN-gamma, IL-12p40, 
      and IL-1beta was significantly higher in the T. gondii-infected BMDCs obtained from 
      the C57BL/6 mice than in those from the BALB/c mice. These findings indicate that 
      differences in the activation status of the BMDCs in the BALB/c and C57BL/6 mice 
      may account for their differential susceptibility to T. gondii.
FAU - Lee, Jae-Hyung
AU  - Lee JH
AD  - Department of Medical Science and Department of Infection Biology, Chungnam 
      National University College of Medicine, Daejeon 35015, Korea.
FAU - Yuk, Jae-Min
AU  - Yuk JM
AD  - Department of Medical Science and Department of Infection Biology, Chungnam 
      National University College of Medicine, Daejeon 35015, Korea.
FAU - Cha, Guang-Ho
AU  - Cha GH
AD  - Department of Medical Science and Department of Infection Biology, Chungnam 
      National University College of Medicine, Daejeon 35015, Korea.
FAU - Lee, Young-Ha
AU  - Lee YH
AD  - Department of Medical Science and Department of Infection Biology, Chungnam 
      National University College of Medicine, Daejeon 35015, Korea.
LA  - eng
PT  - Journal Article
DEP - 20230523
PL  - Korea (South)
TA  - Parasites Hosts Dis
JT  - Parasites, hosts and diseases
JID - 9918574074806676
RN  - 0 (Cytokines)
RN  - 0 (Interleukins)
RN  - 0 (CD40 Antigens)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - *Cytokines/metabolism
MH  - *Toxoplasma
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Interleukins/metabolism
MH  - CD40 Antigens/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Dendritic Cells
PMC - PMC10234828
OTO - NOTNLM
OT  - Toxoplasma gondii
OT  - co-stimulatory molecule
OT  - cytokine
OT  - dendritic cell
OT  - mouse
COIS- The authors declare no conflict of interest related to this study.
EDAT- 2023/06/01 01:08
MHDA- 2023/06/02 06:42
PMCR- 2023/05/01
CRDT- 2023/05/31 21:39
PHST- 2022/10/30 00:00 [received]
PHST- 2023/03/14 00:00 [accepted]
PHST- 2023/06/02 06:42 [medline]
PHST- 2023/06/01 01:08 [pubmed]
PHST- 2023/05/31 21:39 [entrez]
PHST- 2023/05/01 00:00 [pmc-release]
AID - PHD.22150 [pii]
AID - phd-22150 [pii]
AID - 10.3347/PHD.22150 [doi]
PST - ppublish
SO  - Parasites Hosts Dis. 2023 May;61(2):138-146. doi: 10.3347/PHD.22150. Epub 2023 
      May 23.