PMID- 37258751
OWN - NLM
STAT- MEDLINE
DCOM- 20230602
LR  - 20230608
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2675
DP  - 2023
TI  - In Vivo Tissue Lipid Uptake in Antisense Oligonucleotide (ASO)-Treated Mice.
PG  - 1-13
LID - 10.1007/978-1-0716-3247-5_1 [doi]
AB  - The prevalence of obesity has increased to pandemic levels over the past years. 
      Associated comorbidities linked with the accumulation of lipids in different 
      tissues and blood are responsible for the high mortality in these patients. The 
      increased dietary lipid uptake contributes to these metabolic diseases. 
      Identifying which pathways might be dysregulated in these patients will 
      contribute to find new therapeutic targets. Thus, here, a protocol to follow up 
      the distribution of dietary lipids in blood and tissues is provided. For this, 
      radiolabeled triglyceride in olive oil is administered by oral gavage. To 
      ascertain more precisely the capacity of each tissue for fatty acid uptake, not 
      considering the intestinal barrier, the intravenous (IV) administration of 
      radiolabeled lipids is also described.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Aurrekoetxea, Igor
AU  - Aurrekoetxea I
AD  - Faculty of Medicine and Nursing, Department of Physiology, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
AD  - Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.
FAU - Gomez-Santos, Beatriz
AU  - Gomez-Santos B
AD  - Faculty of Medicine and Nursing, Department of Physiology, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Apodaka-Biguri, Maider
AU  - Apodaka-Biguri M
AD  - Faculty of Medicine and Nursing, Department of Physiology, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Ruiz de Gauna, Mikel
AU  - Ruiz de Gauna M
AD  - Faculty of Medicine and Nursing, Department of Physiology, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Gonzalez-Romero, Francisco
AU  - Gonzalez-Romero F
AD  - Faculty of Medicine and Nursing, Department of Physiology, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Buque, Xabier
AU  - Buque X
AD  - Faculty of Medicine and Nursing, Department of Physiology, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Aspichueta, Patricia
AU  - Aspichueta P
AD  - Faculty of Medicine and Nursing, Department of Physiology, University of the 
      Basque Country UPV/EHU, Leioa, Spain. patricia.aspichueta@ehu.eus.
AD  - Biocruces Bizkaia Health Research Institute, Barakaldo, Spain. 
      patricia.aspichueta@ehu.eus.
AD  - National Institute for the Study of Liver and Gastrointestinal Diseases 
      (CIBERehd, Instituto de Salud Carlos III), Madrid, Spain. 
      patricia.aspichueta@ehu.eus.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Dietary Fats)
RN  - 0 (Triglycerides)
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Oligonucleotides, Antisense/genetics
MH  - *Dietary Fats
MH  - Triglycerides/metabolism
MH  - Fatty Acids/metabolism
MH  - Obesity/genetics
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - Lipids
OT  - Metabolism
OT  - Radioactive
OT  - Tissues
OT  - Triglycerides
EDAT- 2023/06/01 01:08
MHDA- 2023/06/02 06:42
CRDT- 2023/05/31 23:28
PHST- 2023/06/02 06:42 [medline]
PHST- 2023/06/01 01:08 [pubmed]
PHST- 2023/05/31 23:28 [entrez]
AID - 10.1007/978-1-0716-3247-5_1 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2675:1-13. doi: 10.1007/978-1-0716-3247-5_1.