PMID- 37258752
OWN - NLM
STAT- MEDLINE
DCOM- 20230602
LR  - 20230608
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2675
DP  - 2023
TI  - In Vivo Hepatic Triglyceride Secretion Rate in Antisense Oligonucleotide 
      (ASO)-Treated Mice.
PG  - 15-26
LID - 10.1007/978-1-0716-3247-5_2 [doi]
AB  - The liver is a central organ in regulating the whole body metabolic homeostasis, 
      and, among many other processes, it plays a crucial role in lipoprotein 
      metabolism. The liver controls the secretion of very-low-density lipoproteins 
      (VLDLs), particles specialized in the transport of liver lipids, mainly 
      triglycerides (TGs), to the adipose tissue, heart, and muscle, among other 
      tissues, providing fatty acids to be stored or to be used as an energy source. 
      The analysis of this metabolic process provides relevant information about the 
      crosstalk between the liver and other organs. It also helps to identify how the 
      liver is able to secrete lipids to reduce its accumulation. This protocol shows 
      how to analyze the liver TG secretion rate blocking the VLDL clearance from the 
      blood by the administration of poloxamer 407. In addition, it shows how to 
      isolate the VLDL produced by the liver at the end of the experiment, so that the 
      apolipoprotein and lipid content and size can be measured. Using antisense 
      oligonucleotides (ASOs) for silencing target proteins involved in metabolic 
      diseases has emerged as a new promising therapeutic approach. Thus, the usage of 
      ASOs has also been included in this protocol. As a conclusion, evaluation of TG 
      secretion rate in mice provides key information to understand the organ crosstalk 
      in metabolic diseases and the capacity of the liver to secrete lipids to blood.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Gomez-Santos, Beatriz
AU  - Gomez-Santos B
AD  - Department of Physiology, Faculty of Medicine and Nursing, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Saenz de Urturi, Diego
AU  - Saenz de Urturi D
AD  - Department of Physiology, Faculty of Medicine and Nursing, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Buque, Xabier
AU  - Buque X
AD  - Department of Physiology, Faculty of Medicine and Nursing, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Aurrekoetxea, Igor
AU  - Aurrekoetxea I
AD  - Department of Physiology, Faculty of Medicine and Nursing, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
AD  - Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.
FAU - Nieva, Ane
AU  - Nieva A
AD  - Department of Physiology, Faculty of Medicine and Nursing, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Fernandez-Puertas, Idoia
AU  - Fernandez-Puertas I
AD  - Department of Physiology, Faculty of Medicine and Nursing, University of the 
      Basque Country UPV/EHU, Leioa, Spain.
FAU - Aspichueta, Patricia
AU  - Aspichueta P
AD  - Department of Physiology, Faculty of Medicine and Nursing, University of the 
      Basque Country UPV/EHU, Leioa, Spain. patricia.aspichueta@ehu.eus.
AD  - Biocruces Bizkaia Health Research Institute, Barakaldo, Spain. 
      patricia.aspichueta@ehu.eus.
AD  - National Institute for the Study of Liver and Gastrointestinal Diseases 
      (CIBERehd, Instituto de Salud Carlos III), Madrid, Spain. 
      patricia.aspichueta@ehu.eus.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Triglycerides)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Lipoproteins, VLDL)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Triglycerides/metabolism
MH  - *Fatty Liver/metabolism
MH  - Oligonucleotides, Antisense/genetics/metabolism
MH  - Liver/metabolism
MH  - Lipoproteins, VLDL/metabolism
OTO - NOTNLM
OT  - Lipid
OT  - Lipoprotein
OT  - Liver
OT  - Metabolism
OT  - Oligonucleotides
OT  - Triglycerides
EDAT- 2023/06/01 01:08
MHDA- 2023/06/02 06:42
CRDT- 2023/05/31 23:28
PHST- 2023/06/02 06:42 [medline]
PHST- 2023/06/01 01:08 [pubmed]
PHST- 2023/05/31 23:28 [entrez]
AID - 10.1007/978-1-0716-3247-5_2 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2675:15-26. doi: 10.1007/978-1-0716-3247-5_2.