PMID- 37258959
OWN - NLM
STAT- MEDLINE
DCOM- 20230602
LR  - 20230606
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2674
DP  - 2023
TI  - Isolation and Purification of Mycobacterial Extracellular Vesicles (EVs).
PG  - 55-60
LID - 10.1007/978-1-0716-3243-7_3 [doi]
AB  - Bacterial extracellular vesicles (EVs) contain numerous active substances that 
      mediate bacterial interactions with their host and with other microbes. Best 
      defined are the EVs from Gram-negative bacteria that have been shown to deliver 
      virulence factors, modulate the immune responses, mediate antibiotic resistance, 
      and also inhibit competitive microbes. Due to the complex cell wall structures of 
      Gram-positive bacteria and mycobacteria, EVs from these bacteria were only 
      recently reported. This protocol describes the isolation of EVs from 
      mycobacteria.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Rao, Komal Umashankar
AU  - Rao KU
AD  - Department of Microbiology, Immunology and Glycobiology, Institution of 
      Laboratory Medicine, Lund University, Lund, Sweden.
FAU - Godaly, Gabriela
AU  - Godaly G
AD  - Department of Microbiology, Immunology and Glycobiology, Institution of 
      Laboratory Medicine, Lund University, Lund, Sweden. gabriela.godaly@med.lu.se.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Virulence Factors)
SB  - IM
MH  - *Mycobacterium/physiology
MH  - Gram-Positive Bacteria
MH  - Virulence Factors/analysis
MH  - *Extracellular Vesicles/chemistry
OTO - NOTNLM
OT  - Extracellular vesicles
OT  - Mycobacterium tuberculosis
OT  - Vesicle isolation
EDAT- 2023/06/01 01:08
MHDA- 2023/06/02 06:42
CRDT- 2023/05/31 23:33
PHST- 2023/06/02 06:42 [medline]
PHST- 2023/06/01 01:08 [pubmed]
PHST- 2023/05/31 23:33 [entrez]
AID - 10.1007/978-1-0716-3243-7_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2674:55-60. doi: 10.1007/978-1-0716-3243-7_3.