PMID- 37264740
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231026
IS  - 1613-6829 (Electronic)
IS  - 1613-6810 (Linking)
VI  - 19
IP  - 40
DP  - 2023 Oct
TI  - Structural Properties and Surface Modification Decided Pharmacokinetic Behavior 
      and Bio-Distribution of DNA Origami Frameworks in Mice.
PG  - e2302932
LID - 10.1002/smll.202302932 [doi]
AB  - This study establishes and validates a series of three dimentional (3D) DNA 
      origami frameworks (DOFs) carrying imaging probes to evaluate their 
      pharmacokinetics and real-time bio-distribution in mice. Three typical DOFs with 
      distinguished structural properties are subjected to mice intravenous injection 
      to systematically investigate their in vivo behaviors. Tracing the radioisotope 
      zirconium-89 ((89) Zr) trapped at the inner space of the frameworks, positron 
      emission tomography (PET) imaging is employed to record the real-time 
      bio-distribution of the structures and acquire their pharmacokinetic parameters 
      in the major metabolic organs. The 3D DOFs show different behavior compared to 
      previous structures, with lower kidney accumulation and higher liver retention. 
      Modifications to the structures, such as exposed ssDNA or polyethylene glycol 
      (PEG) moieties, impact their behavior, but are structure-dependent. The 43 nm 
      icosahedra framework among the DOFs perform the best in liver targeting, with the 
      ssDNA extensions enhancing this tendency. The modification of triantennary 
      N-acetylgalactosamine (GalNAc), further improves its uptake in liver cells, 
      especially in hepatocytes over other cell types, discovered by flow cytometry 
      analysis.
CI  - (c) 2023 Wiley-VCH GmbH.
FAU - Liu, Dunfang
AU  - Liu D
AD  - Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, 
      Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 
      200127, China.
AD  - Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao 
      Tong University, Shanghai, 200127, China.
FAU - Chen, Xiao
AU  - Chen X
AD  - Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, 
      Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 
      200127, China.
FAU - Jiang, Dawei
AU  - Jiang D
AD  - Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology and Hubei Key Laboratory of Molecular 
      Imaging, Wuhan, 430022, China.
FAU - Wang, Cheng
AU  - Wang C
AD  - Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao 
      Tong University, Shanghai, 200127, China.
FAU - Xia, Qian
AU  - Xia Q
AD  - Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, 
      Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 
      200127, China.
AD  - Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao 
      Tong University, Shanghai, 200127, China.
FAU - Yang, Yang
AU  - Yang Y
AUID- ORCID: 0000-0002-2630-6062
AD  - Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, 
      Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 
      200127, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230601
PL  - Germany
TA  - Small
JT  - Small (Weinheim an der Bergstrasse, Germany)
JID - 101235338
RN  - 0 (Radiopharmaceuticals)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - C6V6S92N3C (Zirconium)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Radiopharmaceuticals/chemistry
MH  - *Positron-Emission Tomography/methods
MH  - Polyethylene Glycols/chemistry
MH  - Zirconium/chemistry
MH  - DNA
MH  - Cell Line, Tumor
OTO - NOTNLM
OT  - DNA origami
OT  - bio-distribution
OT  - hepatocyte targeting
OT  - pharmacokinetics
OT  - radioactive labeling
EDAT- 2023/06/02 06:42
MHDA- 2023/10/23 12:42
CRDT- 2023/06/02 03:50
PHST- 2023/05/08 00:00 [revised]
PHST- 2023/04/07 00:00 [received]
PHST- 2023/10/23 12:42 [medline]
PHST- 2023/06/02 06:42 [pubmed]
PHST- 2023/06/02 03:50 [entrez]
AID - 10.1002/smll.202302932 [doi]
PST - ppublish
SO  - Small. 2023 Oct;19(40):e2302932. doi: 10.1002/smll.202302932. Epub 2023 Jun 1.