PMID- 37264745 OWN - NLM STAT- MEDLINE DCOM- 20231023 LR - 20231025 IS - 2192-2659 (Electronic) IS - 2192-2640 (Linking) VI - 12 IP - 25 DP - 2023 Oct TI - 3D Bioprinting of Neurovascular Tissue Modeling with Collagen-Based Low-Viscosity Composites. PG - e2300004 LID - 10.1002/adhm.202300004 [doi] AB - In vitro neurovascular unit (NVU) models are valuable for investigating brain functions and developing drugs. However, it remains challenging to recapitulate the native architectural features and ultra-soft extracellular matrix (ECM) properties of the natural NVU. Cell-laden bioprinting is promising to prepare complex living tissues, but hard to balance the fidelity and cell growth. This study proposes a novel two-stage methodology for biomanufacturing functional 3D neurovascular constructs in vitro with low modulus of ECM. At the shaping stage, a low-viscosity alginate/collagen is printed through an embedded approach; at the culturing stage, the alginate is removed through targeted lysing. The low-viscosity and rapid crosslinking properties provide a printing resolution of approximately 10 microm, and the lysis processing can decrease the hydrogels' modulus to approximately 1 kPa and adjust the porosity of the microstructure, providing cells with an environment closing to the brain ECM. A 3D hollow coaxial neurovascular model is fabricated, in which the endothelial cells has expressed tight junction proteins and shown selective permeability, and the astrocytes outside of the endothelial layer are found to spread out with branches and directly interact with endothelial cells. The present study offers a promising modeling method for better understanding the NVU function and screening neuro-drugs. CI - (c) 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH. FAU - Wang, Sen AU - Wang S AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. FAU - Bai, Luge AU - Bai L AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. FAU - Hu, Xiaoxuan AU - Hu X AD - Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, China. AD - Key Laboratory of Ministry of Education for Environment and Genes Related to Diseases, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, China. FAU - Yao, Siqi AU - Yao S AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. FAU - Hao, Zhiyan AU - Hao Z AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. FAU - Zhou, JiaJia AU - Zhou J AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. FAU - Li, Xiao AU - Li X AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. FAU - Lu, Haixia AU - Lu H AD - Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, China. AD - Key Laboratory of Ministry of Education for Environment and Genes Related to Diseases, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, China. AD - Department of Human Anatomy & Histoembryology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, China. FAU - He, Jiankang AU - He J AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. FAU - Wang, Ling AU - Wang L AUID- ORCID: 0000-0001-5897-8122 AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. FAU - Li, Dichen AU - Li D AD - State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710054, China. AD - NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an, 710054, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230614 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 RN - 0 (Hydrogels) RN - 9007-34-5 (Collagen) RN - 0 (Alginates) SB - IM MH - *Endothelial Cells MH - *Bioprinting/methods MH - Viscosity MH - Hydrogels/chemistry MH - Collagen MH - Alginates/chemistry MH - Printing, Three-Dimensional MH - Tissue Scaffolds MH - Tissue Engineering/methods OTO - NOTNLM OT - 3D bioprinting OT - embedded printing OT - extracellular matrix OT - in vitro neural tissue OT - vascular-like structures EDAT- 2023/06/02 06:43 MHDA- 2023/10/23 12:41 CRDT- 2023/06/02 03:50 PHST- 2023/05/27 00:00 [revised] PHST- 2023/01/01 00:00 [received] PHST- 2023/10/23 12:41 [medline] PHST- 2023/06/02 06:43 [pubmed] PHST- 2023/06/02 03:50 [entrez] AID - 10.1002/adhm.202300004 [doi] PST - ppublish SO - Adv Healthc Mater. 2023 Oct;12(25):e2300004. doi: 10.1002/adhm.202300004. Epub 2023 Jun 14.