PMID- 37267204
OWN - NLM
STAT- MEDLINE
DCOM- 20230620
LR  - 20230620
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 6
IP  - 6
DP  - 2023 Jun 19
TI  - Multi-Color-Emissive Magneto-Luminescent Nanoarchitectures for Targeted 
      Identification of Heterogeneous Exosomes Associated with Lung Cancer Metastasis.
PG  - 2446-2458
LID - 10.1021/acsabm.3c00255 [doi]
AB  - Due to the lack of early detection before metastasis and failure of current 
      therapy to cure the disease, lung cancer contributes to the highest 
      cancer-related mortality worldwide. Tenascin C (TNC) (+) exosomes promote 
      metastasis, amphiregulin (AREG) (+) exosomes are associated with chemotherapy 
      resistance, and programmed cell death ligand-1 (PDL-1) (+) exosomes are 
      associated with immunotherapy resistance, and they are emerging as biomarkers in 
      clinics. However, due to heterogeneity, rapid isolation and multiplex detection 
      of these exosomes are challenging. Herein, we report the design of an 
      antibody-conjugated multi-color (orange, yellow, and green)-emissive carbon dot 
      (CD)-attached cobalt spinel ferrite (CoFe(2)O(4))-based magneto-luminescent 
      nanoarchitecture for targeted capturing and identification of TNC (+), AREG (+), 
      and PDL-1(+) exosomes selectively and simultaneously from whole blood samples. 
      More importantly, to capture and identify the targeted AREG (+) exosome from an 
      infected whole-blood sample, an anti-AREG antibody-attached green (520 
      nm)-emissive CD-conjugated CoFe(2)O(4) nanoparticle-based magnetic-green 
      luminescence nanoarchitecture was developed. Similarly, an anti-PDL-1 
      antibody-attached orange (600 nm)-emissive CDs-based magnetic-orange luminescence 
      nanoarchitecture has been produced to capture and identify the PDL-1 (+) exosome. 
      Furthermore, an anti-TNC antibody-attached yellow (560 nm)-emissive CD-based 
      magnetic-orange luminescent nanoarchitecture has been designed to capture and 
      identify the TNC (+) exosome. Notably, our finding reveals that 100% TNC (+) 
      exosomes can be captured and imaged selectively from an infected blood sample 
      using an anti-TNC antibody-conjugated nanoarchitecture. In addition, 100% AREG 
      (+) exosomes can be captured and imaged selectively using an anti-AREG 
      antibody-conjugated nanoarchitecture. Moreover, 100% PDL-1 (+) exosomes can be 
      captured and imaged selectively using an anti-PDL-1 antibody-conjugated 
      nanoarchitecture. Furthermore, we have demonstrated that a multi-color-emissive 
      nanoarchitecture can be used for capturing and imaging all three exosomes 
      simultaneously.
FAU - Pramanik, Avijit
AU  - Pramanik A
AUID- ORCID: 0000-0002-4623-2099
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
FAU - Kundu, Sanchita
AU  - Kundu S
AUID- ORCID: 0000-0002-9843-0476
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
FAU - Gates, Kaelin
AU  - Gates K
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
FAU - Kumar, Animesh
AU  - Kumar A
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
FAU - Kolawole, Olorunsola Praise
AU  - Kolawole OP
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
FAU - Talukdar, Arohan
AU  - Talukdar A
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
FAU - Iftekhar, Ridwan
AU  - Iftekhar R
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
FAU - Corby, Lauren R
AU  - Corby LR
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
FAU - Ray, Paresh Chandra
AU  - Ray PC
AUID- ORCID: 0000-0001-5398-9930
AD  - Department of Chemistry and Biochemistry, Jackson State University, Jackson, 
      Mississippi 39217, United States.
LA  - eng
GR  - U54 MD015929/MD/NIMHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20230602
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
RN  - 0 (Biomarkers)
SB  - IM
MH  - Humans
MH  - *Exosomes/metabolism
MH  - Luminescence
MH  - *Lung Neoplasms/metabolism
MH  - Biomarkers/metabolism
MH  - *Nanoparticles
OTO - NOTNLM
OT  - antibody-conjugated magnetic-luminescent nanoplatforms
OT  - identification of heterogeneous exosomes
OT  - lung cancer metastasis
OT  - multi-color-emissive carbon dots
OT  - selective exosome separation
EDAT- 2023/06/02 19:13
MHDA- 2023/06/20 06:42
CRDT- 2023/06/02 13:23
PHST- 2023/06/20 06:42 [medline]
PHST- 2023/06/02 19:13 [pubmed]
PHST- 2023/06/02 13:23 [entrez]
AID - 10.1021/acsabm.3c00255 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2023 Jun 19;6(6):2446-2458. doi: 10.1021/acsabm.3c00255. Epub 
      2023 Jun 2.