PMID- 37271505
OWN - NLM
STAT- MEDLINE
DCOM- 20230606
LR  - 20230610
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 24
IP  - 3
DP  - 2023 May
TI  - In vitro effects of monophosphoryl lipid A and Poly I:C combination on equine 
      cells.
PG  - e37
LID - 10.4142/jvs.23007 [doi]
LID - e37
AB  - BACKGROUND: Toll-like receptor (TLR) agonists have been used as adjuvants to 
      modulate immune responses in both animals and humans. OBJECTIVES: The objective 
      of this study was to evaluate the combined effects of the TLR 4 agonist 
      monophosphoryl lipid A (MPL) and the TLR 3 agonist polyinosinic:polycytidylic 
      acid (Poly I:C) on equine peripheral blood mononuclear cells (PBMCs), 
      monocyte-derived dendritic cells (MoDCs), and bone marrow-derived mesenchymal 
      stromal cells (BM-MSCs). METHODS: The PBMCs, MoDCs, and BM-MSCs collected from 
      three mixed breed horses were treated with MPL, Poly I:C, and their combination. 
      The mRNA expression of interferon gamma (IFN-gamma), interleukin (IL)-1beta, IL-4, IL-6, 
      IL-8, IL-12p40, tumor necrosis factor alpha (TNF-alpha), vascular endothelial growth 
      factor (VEGF), and monocyte chemoattractant protein-1 (MCP-1) was determined 
      using real-time polymerase chain reaction. RESULTS: The combination of MPL and 
      Poly I:C significantly upregulated immunomodulatory responses in equine cells/ 
      without cytotoxicity. The combination induced greater mRNA expression of 
      pro-inflammatory cytokines IFN-gamma and IL-6 than MPL or Poly I:C stimulation alone 
      in PBMCs. In addition, the combination induced significantly higher mRNA 
      expression of IL-1beta, IL-6, and IL-12p40 in MoDCs, and IL-8, MCP-1, and VEGF in 
      BM-MSCs compared to stimulation with a single TLR agonist. CONCLUSIONS: The 
      combination of MPL and Poly I:C can be used as a potential adjuvant candidate for 
      vaccines to aid in preventing infectious diseases in horses.
CI  - (c) 2023 The Korean Society of Veterinary Science.
FAU - Lee, Dong-Ha
AU  - Lee DH
AUID- ORCID: 0000-0002-8898-3632
AD  - College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju 
      National University, Jeju 63243, Korea.
FAU - Lee, Eun-Bee
AU  - Lee EB
AUID- ORCID: 0000-0003-3654-5654
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National 
      University, Jeju 63243, Korea.
FAU - Seo, Jong-Pil
AU  - Seo JP
AUID- ORCID: 0000-0002-6418-9813
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National 
      University, Jeju 63243, Korea.
FAU - Ko, Eun-Ju
AU  - Ko EJ
AUID- ORCID: 0000-0002-1081-904X
AD  - College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju 
      National University, Jeju 63243, Korea.
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National 
      University, Jeju 63243, Korea. eunju@jejunu.ac.kr.
LA  - eng
GR  - Jeju National University/Korea
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - MWC0ET1L2P (monophosphoryl lipid A)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Interleukin-8)
RN  - 0 (Cytokines)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (RNA, Messenger)
RN  - 25249-22-3 (Poly I)
SB  - IM
MH  - Humans
MH  - Horses/genetics
MH  - Animals
MH  - *Leukocytes, Mononuclear/metabolism
MH  - *Vascular Endothelial Growth Factor A
MH  - Interleukin-6
MH  - Interleukin-12 Subunit p40
MH  - Interleukin-8
MH  - Cytokines/genetics/metabolism
MH  - Interferon-gamma
MH  - Adjuvants, Immunologic/pharmacology
MH  - RNA, Messenger
MH  - Poly I
PMC - PMC10244135
OTO - NOTNLM
OT  - Toll-Like Receptors
OT  - bone marrow stromal cell
OT  - dendritic cells
OT  - immunomodulation
OT  - peripheral blood mononuclear cell
COIS- The authors declare no conflicts of interest.
EDAT- 2023/06/05 00:42
MHDA- 2023/06/06 06:42
PMCR- 2023/05/01
CRDT- 2023/06/04 20:52
PHST- 2023/01/04 00:00 [received]
PHST- 2023/02/09 00:00 [revised]
PHST- 2023/03/10 00:00 [accepted]
PHST- 2023/06/06 06:42 [medline]
PHST- 2023/06/05 00:42 [pubmed]
PHST- 2023/06/04 20:52 [entrez]
PHST- 2023/05/01 00:00 [pmc-release]
AID - 24.e37 [pii]
AID - 10.4142/jvs.23007 [doi]
PST - ppublish
SO  - J Vet Sci. 2023 May;24(3):e37. doi: 10.4142/jvs.23007.