PMID- 37272437
OWN - NLM
STAT- MEDLINE
DCOM- 20230606
LR  - 20230606
IS  - 0004-1955 (Print)
IS  - 0004-1955 (Linking)
VI  - 85
IP  - 3
DP  - 2023
TI  - [Detection of SARS-CoV-2 RNA in the mucosa of the appendices of children with 
      COVID-19].
PG  - 23-28
LID - 10.17116/patol20238503123 [doi]
AB  - Background. The novel coronavirus infection (COVID-19) often manifests in 
      children as diarrhea, vomiting, abdominal pain, and some children develop acute 
      appendicitis. To elucidate the role of SARS-CoV-2 in the development of acute 
      appendicitis, a more detailed study of the presence of its genetic material in 
      the tissue of the appendix. OBJECTIVE: Determination of SARS-CoV-2 RNA in 
      appendices of children with COVID-19 by fluorescence in situ hybridization 
      (FISH). MATERIAL AND METHODS: A retrospective analysis of case histories and 
      morphological analysis using FISH of appendices of pediatric patients with 
      established clinical diagnosis of acute appendicitis and confirmed infection with 
      SARS-CoV-2 was performed. The material was divided into 3 groups: 1st -appendices 
      obtained during appendectomy in children with established clinical diagnosis of 
      <<coronavirus infection>> (COVID-19, PCR+) (n=42; mean age 10.8 years); 2nd - 
      appendices of children (n=55; mean age 9.7 years) with acute appendicitis 
      obtained before the onset of the COVID-19 pandemic; 3rd (control) group (n=38; 
      mean age 10.3 years) - autopsy material of the appendices (intact). RESULTS: In 
      all samples of the appendices of the 1st group, a positive SARS-CoV-2 viral RNA 
      signal was noted in the cytoplasm of most epithelial cells and single 
      immunocompetent cells. The signal intensity remained the same in all slides, 
      regardless of age. In all samples obtained from patients without COVID-19 (groups 
      2 and 3), confocal microscopy did not reveal a signal, which indicates successful 
      adaptation of the FISH method in this study and excludes the false positive 
      results. CONCLUSION: In the epithelium of the appendices of children of different 
      age with COVID-19, the FISH method revealed SARS-CoV-2 RNA, which does not 
      exclude the association between viral invasion and the development of acute 
      appendicitis.
FAU - Demyashkin, G A
AU  - Demyashkin GA
AUID- ORCID: 0000-0001-8447-2600
AD  - National Medical Research Centre of Radiology, Obninsk, Russia.
AD  - I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
FAU - Gorokhov, K R
AU  - Gorokhov KR
AUID- ORCID: 0000-0001-5252-0436
AD  - National Medical Research Centre of Radiology, Obninsk, Russia.
FAU - Nikitin, P V
AU  - Nikitin PV
AUID- ORCID: 0000-0003-3223-4584
AD  - I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
FAU - Zorin, I A
AU  - Zorin IA
AUID- ORCID: 0000-0002-1621-7015
AD  - I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
FAU - Voznesenskaya, S I
AU  - Voznesenskaya SI
AUID- ORCID: 0000-0003-3473-9797
AD  - I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Vyyavlenie RNK SARS-CoV-2 v slizistoi obolochke cherveobraznogo otrostka u detei 
      s COVID-19.
PL  - Russia (Federation)
TA  - Arkh Patol
JT  - Arkhiv patologii
JID - 0370604
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Child
MH  - Humans
MH  - *Appendix
MH  - *Appendicitis/diagnosis/genetics/surgery
MH  - *COVID-19/diagnosis
MH  - SARS-CoV-2/genetics
MH  - RNA, Viral/genetics
MH  - Retrospective Studies
MH  - Pandemics
MH  - In Situ Hybridization, Fluorescence
MH  - Mucous Membrane
OTO - NOTNLM
OT  - FISH
OT  - SARS-CoV-2
OT  - appendicitis
OT  - children
EDAT- 2023/06/05 13:05
MHDA- 2023/06/06 06:42
CRDT- 2023/06/05 06:11
PHST- 2023/06/06 06:42 [medline]
PHST- 2023/06/05 13:05 [pubmed]
PHST- 2023/06/05 06:11 [entrez]
AID - 10.17116/patol20238503123 [doi]
PST - ppublish
SO  - Arkh Patol. 2023;85(3):23-28. doi: 10.17116/patol20238503123.