PMID- 37272786
OWN - NLM
STAT- MEDLINE
DCOM- 20230710
LR  - 20231119
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 325
IP  - 2
DP  - 2023 Aug 1
TI  - Hematoloechus sp. attachment shifts endothelium in vivo from pro- to 
      anti-inflammatory profile in Rana pipiens: evidence from systemic and capillary 
      physiology.
PG  - R133-R153
LID - 10.1152/ajpregu.00041.2023 [doi]
AB  - This prospective, descriptive study focused on lung flukes (Hematoloechus sp., H) 
      and their impact on systemic and individual capillary variables measured in 
      pithed Rana pipiens, a long-standing model for studies of capillary physiology. 
      Three groups were identified based on Hematoloechus attachment: no Hematoloechus 
      (No H), Hematoloechus not attached (H Not Att), and Hematoloechus attached (H 
      Att). Among 38 descriptive, cardiovascular, and immunological variables, 18 
      changed significantly with H. Symptoms of H included weight loss, elevated immune 
      cells, heart rate variability, faster coagulation, lower hematocrit, and fluid 
      accumulation. Important capillary function discoveries included median baselines 
      for hydraulic conductivity (L(p)) of 7.0 (No H), 12.4 (H Not Att), and 4.2 (H 
      Att) x 10(-7) cm.s(-1).cmH(2)O(-1) (P < 0.0001) plus seasonal adaptation of sigma 
      delta pi [sigma(pi(c)-pi(i)), P = 0.03]. Pro- and anti-inflammatory phases were 
      revealed for L(p) and plasma nitrite/nitrate concentration ([NO(x)]) in both H 
      Not Att and H Att, whereas capillary wall tensile strength increased in the H 
      Att. H attachment was advantageous for the host due to lower edema and for the 
      parasite via a sustained food source illustrating an excellent example of natural 
      symbiosis. However, H attachment also resulted in host weight loss: in time, a 
      conundrum for the highly dependent parasite. The study increases overall 
      knowledge of Rana pipiens by revealing intriguing effects of H and previously 
      unknown, naturally occurring seasonal changes in many variables. The data improve 
      Rana pipiens as a general scientific and capillary physiology model. Diseases of 
      inflammation and stroke are among the clinical applications.
FAU - Williams, Donna A
AU  - Williams DA
AUID- ORCID: 0000-0002-4706-4760
AD  - College of Nursing, Montana State University, Bozeman, Montana, United States.
FAU - Flood, Mary H
AU  - Flood MH
AD  - College of Nursing, Montana State University, Bozeman, Montana, United States.
LA  - eng
GR  - R01 HL63125/HHS | National Institutes of Health (NIH)/
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20230605
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - Animals
MH  - Rana pipiens
MH  - Prospective Studies
MH  - *Capillaries/physiology
MH  - Endothelium
MH  - *Anti-Inflammatory Agents
MH  - Capillary Permeability/physiology
PMC - PMC10393331
OTO - NOTNLM
OT  - hydration
OT  - lower vertebrates
OT  - nutrition
OT  - parasitology
OT  - pulmonary
COIS- No conflicts of interest, financial or otherwise, are declared by the authors.
EDAT- 2023/06/05 13:04
MHDA- 2023/07/10 06:42
PMCR- 2023/06/05
CRDT- 2023/06/05 09:53
PHST- 2023/07/10 06:42 [medline]
PHST- 2023/06/05 13:04 [pubmed]
PHST- 2023/06/05 09:53 [entrez]
PHST- 2023/06/05 00:00 [pmc-release]
AID - R-00041-2023 [pii]
AID - 10.1152/ajpregu.00041.2023 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2023 Aug 1;325(2):R133-R153. doi: 
      10.1152/ajpregu.00041.2023. Epub 2023 Jun 5.