PMID- 37273978
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230606
IS  - 2424-8002 (Electronic)
IS  - 2424-7723 (Print)
IS  - 2424-8002 (Linking)
VI  - 9
IP  - 3
DP  - 2023
TI  - 3D-printed dual drug delivery nanoparticle- loaded hydrogels to combat 
      antibiotic-resistant bacteria.
PG  - 683
LID - 10.18063/ijb.683 [doi]
LID - 683
AB  - Implant-associated infections are not easy to diagnose and very difficult to 
      treat, due to the ability of major pathogens, such as Staphylococcus aureus, to 
      develop biofilms and escape the immune response and antibiotic treatment. We, 
      therefore, aimed to develop a 3D-printed dual rifampicin (Rif)- and vancomycin 
      (Van)-loaded polylactic- co-glycolic acid (PLGA) nanoparticles (NPs) delivery 
      system based on hydrogels made of gelatin methacrylate (GelMA). The release of 
      Rif and Van from NPs manufactured from different PLGA molecular weights was 
      studied in phosphate-buffered saline for 21 days. Low molecular weight PLGA NPs 
      exhibited the fastest release of Rif and Van within the first 7 days and were 
      selected for antimicrobial evaluation. Four different GelMA-based 3D-printed 
      samples were successfully produced, carrying non-loaded NPs, Rif-NPs, Van-NPs, or 
      alternating layers of Rif-NPs and Van-NP. The exposition of S. aureus against 
      increased concentrations of Rif or Van produced new resistant strains to Rif 
      (Rif(R)) or Van (Van(R)). The GelMA hydrogel co-delivering Rif and Van eradicated 
      S. aureus RN4220 Rif(R) and RN4220 Van(R) strains. S. aureus RN4220 and S. aureus 
      AMC 201 colonies developed resistance to Rif after contact with the GelMA 
      hydrogel containing only Rif-NPs which appeared to be due to known mutations in 
      the rpoB gene. In conclusion, 3D-printed GelMA hydrogel loaded with PLGA 
      Rif-Van-NPs drug delivery system show promising in vitro results to prevent 
      implant-associated infections caused by antimicrobial-resistant bacteria.
CI  - Copyright: (c) 2023 Martinez-Perez D, Guarch-Perez C, Purbayanto MAK, et al.
FAU - Martinez-Perez, David
AU  - Martinez-Perez D
AD  - Faculty of Materials Science and Engineering, Warsaw University of Technology, 
      Woloska 141, 02-507, Warsaw, Poland.
FAU - Guarch-Perez, Clara
AU  - Guarch-Perez C
AD  - Department of Medical Microbiology and Infection Prevention, Amsterdam Institute 
      for Infection and Immunity, Amsterdam UMC, University of Amsterdam, Meibergdreef 
      9, 1105 AZ, Amsterdam, the Netherlands.
FAU - Purbayanto, Muhammad Abiyyu Kenichi
AU  - Purbayanto MAK
AD  - Faculty of Materials Science and Engineering, Warsaw University of Technology, 
      Woloska 141, 02-507, Warsaw, Poland.
FAU - Choinska, Emilia
AU  - Choinska E
AD  - Faculty of Materials Science and Engineering, Warsaw University of Technology, 
      Woloska 141, 02-507, Warsaw, Poland.
FAU - Riool, Martijn
AU  - Riool M
AD  - Department of Medical Microbiology and Infection Prevention, Amsterdam Institute 
      for Infection and Immunity, Amsterdam UMC, University of Amsterdam, Meibergdreef 
      9, 1105 AZ, Amsterdam, the Netherlands.
FAU - Zaat, Sebastian A J
AU  - Zaat SAJ
AD  - Department of Medical Microbiology and Infection Prevention, Amsterdam Institute 
      for Infection and Immunity, Amsterdam UMC, University of Amsterdam, Meibergdreef 
      9, 1105 AZ, Amsterdam, the Netherlands.
FAU - Wojciech, Swieszkowski
AU  - Wojciech S
AD  - Faculty of Materials Science and Engineering, Warsaw University of Technology, 
      Woloska 141, 02-507, Warsaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20230213
PL  - Singapore
TA  - Int J Bioprint
JT  - International journal of bioprinting
JID - 101709763
PMC - PMC10236340
OTO - NOTNLM
OT  - 3D printing
OT  - Antibiotic resistance
OT  - Controlled drug delivery
OT  - Gelatin methacrylate
OT  - Nanoparticles
OT  - Staphylococcus aureus
COIS- The authors declare no conflicts of interest.
EDAT- 2023/06/05 13:04
MHDA- 2023/06/05 13:05
PMCR- 2023/02/13
CRDT- 2023/06/05 11:55
PHST- 2022/10/04 00:00 [received]
PHST- 2022/11/08 00:00 [accepted]
PHST- 2023/06/05 13:05 [medline]
PHST- 2023/06/05 13:04 [pubmed]
PHST- 2023/06/05 11:55 [entrez]
PHST- 2023/02/13 00:00 [pmc-release]
AID - IJB-9-3-683 [pii]
AID - 10.18063/ijb.683 [doi]
PST - epublish
SO  - Int J Bioprint. 2023 Feb 13;9(3):683. doi: 10.18063/ijb.683. eCollection 2023.