PMID- 37277556
OWN - NLM
STAT- MEDLINE
DCOM- 20230922
LR  - 20230922
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 48
IP  - 11
DP  - 2023 Nov
TI  - Baicalein Attenuates Neuroinflammation in LPS-Treated BV-2 Cells by Inhibiting 
      Glycolysis via STAT3/c-Myc Pathway.
PG  - 3363-3377
LID - 10.1007/s11064-023-03961-5 [doi]
AB  - More and more evidence shows that metabolic reprogramming is closely related to 
      the occurrence of AD. The metabolic conversion of oxidative phosphorylation into 
      glycolysis will aggravate microglia-mediated inflammation. It has been 
      demonstrated that baicalein could inhibit neuroinflammation in LPS-treated BV-2 
      microglial cells, but whether the anti-neuroinflammatory mechanisms of baicalein 
      were related to glycolysis is unclear. Our results depicted that baicalein 
      significantly inhibited the levels of nitric oxide (NO), interleukin-6 (IL-6), 
      prostaglandin 2 (PGE2) and tumor necrosis factor (TNF-alpha) in LPS-treated BV-2 
      cells. (1)H-NMR metabolomics analysis showed that baicalein decreased the levels 
      of lactic acid and pyruvate, and significantly regulated glycolytic pathway. 
      Further study revealed that baicalein significantly inhibited the activities of 
      glycolysis-related enzymes including hexokinase (HK), 6-phosphate kinase (6-PFK), 
      pyruvate kinase (PK), lactate dehydrogenase (LDH), and inhibited STAT3 
      phosphorylation and c-Myc expression. By using of STAT3 activator RO8191, we 
      found that baicalein suppressed the increase of STAT3 phosphorylation and c-Myc 
      expression triggered by RO8191, and inhibited the increased levels of 6-PFK, PK 
      and LDH caused by RO8191. In conclusion, these results suggested that baicalein 
      attenuated the neuroinflammation in LPS-treated BV-2 cells by inhibiting 
      glycolysis through STAT3/c-Myc pathway.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Gao, Li
AU  - Gao L
AD  - Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of 
      Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi 
      University, No. 92, Wucheng Road Xiaodain District, Taiyuan, 030006, Shanxi, 
      China. gaoli87@sxu.edu.cn.
AD  - Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi 
      Province, Taiyuan, 030006, Shanxi, China. gaoli87@sxu.edu.cn.
FAU - Liu, Yu-Xin
AU  - Liu YX
AD  - Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of 
      Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi 
      University, No. 92, Wucheng Road Xiaodain District, Taiyuan, 030006, Shanxi, 
      China.
AD  - Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi 
      Province, Taiyuan, 030006, Shanxi, China.
FAU - Zhou, Yu-Zhi
AU  - Zhou YZ
AD  - Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of 
      Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi 
      University, No. 92, Wucheng Road Xiaodain District, Taiyuan, 030006, Shanxi, 
      China.
AD  - Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi 
      Province, Taiyuan, 030006, Shanxi, China.
FAU - Qin, Xue-Mei
AU  - Qin XM
AD  - Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of 
      Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi 
      University, No. 92, Wucheng Road Xiaodain District, Taiyuan, 030006, Shanxi, 
      China. qinxm@sxu.edu.cn.
AD  - Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi 
      Province, Taiyuan, 030006, Shanxi, China. qinxm@sxu.edu.cn.
LA  - eng
GR  - 202203021211292/Fundamental Research Program of Shanxi Province,China/
GR  - 81603319/National Natural Science Foundation of China, China/
PT  - Journal Article
DEP - 20230605
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 49QAH60606 (baicalein)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Flavanones)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (NF-kappa B)
RN  - 0 (STAT3 protein, human)
RN  - 0 (STAT3 Transcription Factor)
SB  - IM
MH  - Humans
MH  - *Lipopolysaccharides/toxicity
MH  - Neuroinflammatory Diseases
MH  - *Flavanones/pharmacology/therapeutic use
MH  - Inflammation/drug therapy/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Microglia/metabolism
MH  - NF-kappa B/metabolism
MH  - STAT3 Transcription Factor/metabolism
OTO - NOTNLM
OT  - Baicalein
OT  - Glycolysis
OT  - Microglia
OT  - STAT3
EDAT- 2023/06/06 01:11
MHDA- 2023/09/22 06:42
CRDT- 2023/06/05 23:23
PHST- 2022/12/27 00:00 [received]
PHST- 2023/05/23 00:00 [accepted]
PHST- 2023/05/08 00:00 [revised]
PHST- 2023/09/22 06:42 [medline]
PHST- 2023/06/06 01:11 [pubmed]
PHST- 2023/06/05 23:23 [entrez]
AID - 10.1007/s11064-023-03961-5 [pii]
AID - 10.1007/s11064-023-03961-5 [doi]
PST - ppublish
SO  - Neurochem Res. 2023 Nov;48(11):3363-3377. doi: 10.1007/s11064-023-03961-5. Epub 
      2023 Jun 5.