PMID- 372792
OWN - NLM
STAT- MEDLINE
DCOM- 19790629
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 60
IP  - 1
DP  - 1979 Mar
TI  - Photodynamic effects of dyes on bacteria. III. Mutagenesis by acridine orange and 
      500-nm monochromatic light in strains of Escherichia coli that differ in repair 
      capability.
PG  - 1-11
AB  - In the presence of acridine orange (AO) and monochromatic 500-nm light, the 
      recombination-deficient strain of Escherichia coli, WP10 (recA), showed a 15-fold 
      increase in mutation rate over the wild-type (WP2) strain. Under the same 
      conditions, strain Bs--1 (uvrB lexA lon) showed a 5-fold increase in mutation 
      rate over strain WP2. In contrast, the endonuclease-deficient, strain, WP2s 
      (uvrA), showed a lower AO-500 nm mutation rate than wild-type. The extremely high 
      mutation rate of the recA strain cannot be due to error-prone inducible SOS 
      repair since the inducible recA + function is absent. Repair of the AO-500 
      nm-induced lesions is likely due to a recA+-dependent, error-free, recombination 
      process. It is concluded that the high mutation rates with AO-500 nm light 
      obtained in chemostat cultures of recA and lexA strains occur as a consequence of 
      errors during semi-conservative DNA replication in the presence of unrepaired DNA 
      lesions.
FAU - Hass, B S
AU  - Hass BS
FAU - Webb, R B
AU  - Webb RB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA, Bacterial)
RN  - 0 (Mutagens)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - *DNA Repair
MH  - DNA Replication
MH  - DNA, Bacterial/genetics/*radiation effects
MH  - Escherichia coli/drug effects/*genetics/radiation effects
MH  - *Light
MH  - Mutagens
MH  - *Mutation
MH  - Recombination, Genetic
EDAT- 1979/03/01 00:00
MHDA- 1979/03/01 00:01
CRDT- 1979/03/01 00:00
PHST- 1979/03/01 00:00 [pubmed]
PHST- 1979/03/01 00:01 [medline]
PHST- 1979/03/01 00:00 [entrez]
AID - 0027-5107(79)90204-5 [pii]
AID - 10.1016/0027-5107(79)90204-5 [doi]
PST - ppublish
SO  - Mutat Res. 1979 Mar;60(1):1-11. doi: 10.1016/0027-5107(79)90204-5.