PMID- 37282325
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230607
IS  - 1440-169X (Electronic)
IS  - 0012-1592 (Linking)
VI  - 37
IP  - 4
DP  - 1995 Aug
TI  - Immunohistochemical localization and possible physiological roles of tumor 
      necrosis factor-alpha in mouse cumulus-oocyte complexes.
PG  - 413-420
LID - 10.1046/j.1440-169X.1995.t01-3-00008.x [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha), a cytokine which is produced by activated 
      macrophages, has been shown to participate in the regulation of ovarian 
      functions. In the course of our investigation on the mechanism of maturation, 
      fertilization and degeneration of mouse oocytes, immunoreactivity to TNF-alpha was 
      found in the cytoplasm of the cells surrounding the maturing oocytes and of 
      granulosa cells facing the antral cavity. Immunoblot analysis with the specific 
      antibody to TNF-alpha identified the 17 kDa Mr band in the extract of cumulusoocyte 
      complexes. Various concentrations of TNF-alpha (mouse, recombinant) and anti TNF-alpha 
      antiserum (polyclonal rabbit anti-mouse recombinant TNF-alpha) were then used to 
      determine their effect on the germinal vesicle breakdown (GVBD), polar body 
      extrusion, fertilization and fragmentation of mouse oocytes/eggs. TNF-alpha at 
      concentrations of 10 ng/mL or less and anti-TNF-alpha antiserum at concentrations of 
      10% or less, had no effect on the spontaneous GVBD and polar body extrusion of 
      mouse oocytes in culture. Mouse follicular oocytes cultured for more than 72 h in 
      modified Krebs-Ringer solution in vitro undergo spontaneous fragmentation, which 
      is a degenerative change to form 'blastomeres' with or without nuclear fragments 
      or chromatin. Ghost-like blastomeres were also identified in the space among 
      fragmented 'blastomeres'. The spontaneous fragmentation of mouse follicular 
      oocytes was suppressed in the presence of TNF-alpha at concentrations of 1 ng/mL or 
      greater. Anti-TNF-alpha antiserum (1%) accelerated the induction of fragmentation of 
      oocytes cultured in vitro. The addition of anti TNF-alpha antiserum (10%) to the 
      culture medium did not influence the fertilization rates of the eggs surrounded 
      by the expanded cumulus. These results appear to indicate that the process of 
      degeneration of mouse oocytes/eggs is modulated by TNF-alpha accumulated in the 
      expanded cumulus during oocyte maturation.
FAU - Sato, Eimei
AU  - Sato E
AD  - Department of Reproductive and Developmental Biology, The Institute of Medical 
      Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108, 
      Japan.
FAU - Nakayama, Taisuke
AU  - Nakayama T
AD  - Department of Reproductive and Developmental Biology, The Institute of Medical 
      Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108, 
      Japan.
FAU - Kamio, Kae
AU  - Kamio K
AD  - Department of Applied Biology, Nihon University, 1866, Kameino, Fujisawa 252, 
      Japan.
FAU - Takahashi, Yuji
AU  - Takahashi Y
AD  - Department of Reproductive and Developmental Biology, The Institute of Medical 
      Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108, 
      Japan.
FAU - Toyoda, Yutaka
AU  - Toyoda Y
AD  - Department of Reproductive and Developmental Biology, The Institute of Medical 
      Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108, 
      Japan.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Dev Growth Differ
JT  - Development, growth & differentiation
JID - 0356504
SB  - IM
OTO - NOTNLM
OT  - cumulus-oocyte complexes
OT  - in vitro fertilization
OT  - mouse
OT  - oocyte maturation
OT  - tumor necrosis factor-alpha
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 2023/06/07 02:11
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [pubmed]
PHST- 2023/06/07 02:11 [entrez]
AID - 10.1046/j.1440-169X.1995.t01-3-00008.x [doi]
PST - ppublish
SO  - Dev Growth Differ. 1995 Aug;37(4):413-420. doi: 
      10.1046/j.1440-169X.1995.t01-3-00008.x.