PMID- 37283897
OWN - NLM
STAT- MEDLINE
DCOM- 20230609
LR  - 20230611
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 11
DP  - 2023
TI  - Clinical diagnostic value of amino acids in laryngeal squamous cell carcinomas.
PG  - e15469
LID - 10.7717/peerj.15469 [doi]
LID - e15469
AB  - BACKGROUND: Early diagnosis and treatment can improve the survival rates of 
      patients with laryngeal squamous cell carcinoma (LSCC). Therefore, it is 
      necessary to discover new biomarkers for laryngeal cancer screening and early 
      diagnosis. METHODS: We collected fasting plasma from LSCC patients and healthy 
      volunteers, as well as cancer and para-carcinoma tissues from LSCC patients, and 
      performed quantitative detection of amino acid levels using liquid 
      chromatography-mass spectrometry. We used overall analysis and multivariate 
      statistical analysis to screen out the statistically significant differential 
      amino acids in the plasma and tissue samples, conducted receiver operating 
      characteristic (ROC) analysis of the differential amino acids to evaluate the 
      sensitivity and specificity of the differential amino acids, and finally 
      determined the diagnostic value of amino acids for laryngeal cancer. 
      Additionally, we identified amino acids in the plasma and tissue samples that are 
      valuable for the early diagnosis of laryngeal cancer classified according to the 
      tumor-node-metastasis (TNM) classification. RESULTS: Asparagine (Asp) and 
      homocysteine (Hcy) were two amino acids of common significance in plasma and 
      tissue samples, and their specificity and sensitivity analysis showed that they 
      may be new biomarkers for the diagnosis and treatment of LSCC. According to the 
      TNM staging system, phenylalanine (Phe) and isoleucine (Ile) were screened out in 
      the plasma of LSCC patients at early (I and II) and advanced (III and IV) stages; 
      ornithine hydrochloride (Orn), glutamic acid (Glu), and Glycine (Gly) were 
      selected in the tissue. These dysregulated amino acids found in LSCC patients may 
      be useful as clinical biomarkers for the early diagnosis and screening of LSCC.
CI  - (c) 2023 Hu et al.
FAU - Hu, Shousen
AU  - Hu S
AD  - Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital 
      of Zhengzhou University, Zhengzhou, China.
FAU - Zhao, Chang
AU  - Zhao C
AD  - Department of Otorhinolaryngology, Zhengzhou Seventh People's Hospital, 
      Zhengzhou, China.
FAU - Wang, Zi'an
AU  - Wang Z
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
AD  - Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou University, 
      Zhengzhou, China.
FAU - Li, Zeyun
AU  - Li Z
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
AD  - Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou University, 
      Zhengzhou, China.
FAU - Kong, Xiangzhen
AU  - Kong X
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
AD  - Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou University, 
      Zhengzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230601
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
RN  - 0 (Amino Acids)
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/diagnosis
MH  - *Laryngeal Neoplasms/diagnosis
MH  - *Carcinoma, Squamous Cell/diagnosis
MH  - Amino Acids
MH  - Biomarkers, Tumor
MH  - Early Detection of Cancer
MH  - *Head and Neck Neoplasms
PMC - PMC10239616
OTO - NOTNLM
OT  - Amino acid
OT  - Biomarker
OT  - LC-MS
OT  - Laryngeal cancer
OT  - Staging
COIS- The authors declare that they have no competing interests.
EDAT- 2023/06/07 13:10
MHDA- 2023/06/09 06:42
PMCR- 2023/06/01
CRDT- 2023/06/07 09:39
PHST- 2023/01/20 00:00 [received]
PHST- 2023/05/05 00:00 [accepted]
PHST- 2023/06/09 06:42 [medline]
PHST- 2023/06/07 13:10 [pubmed]
PHST- 2023/06/07 09:39 [entrez]
PHST- 2023/06/01 00:00 [pmc-release]
AID - 15469 [pii]
AID - 10.7717/peerj.15469 [doi]
PST - epublish
SO  - PeerJ. 2023 Jun 1;11:e15469. doi: 10.7717/peerj.15469. eCollection 2023.