PMID- 37288149
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230610
IS  - 2364-3420 (Electronic)
IS  - 2364-3439 (Print)
IS  - 2364-3439 (Linking)
VI  - 7
IP  - 2
DP  - 2021 Apr 30
TI  - Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells.
PG  - 142-151
LID - 10.52601/bpr.2021.200046 [doi]
AB  - Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent and 
      functional in many biological processes, by which releasing secretary factors to 
      promote the self-repairing of damaged tissue or developing into functional cell 
      at local organ. However, there is a high risk that oxidative stress would reduce 
      the pluripotency and factor-secretion during the preparation and transplantation. 
      Therefore, reducing oxidative stress is expected to improve the efficacy of 
      HUC-MSCs therapy. Zinc (Zn) is an essential trace element which involves in the 
      resistance of oxidative stress. To investigate Zn-regulated signaling pathways, 
      we have profiled the gene expression at transcriptome level in primary HUC-MSCs 
      treated with zinc sulfate, followed with GO and KEGG gene enrichment analysis. Zn 
      treatment improved signal pathways for mineral absorption, cell growth, and cell 
      death. Zn deficiency was mimicked by TPEN administration, which suppressed cell 
      proliferation and reduced the expression of HUC-MSCs surface stem cell markers 
      CD73, CD90 and CD105 by flow cytometry. Nuclear factor erythrocyte 2 related 
      factor 2 (Nrf2) plays an important role in antioxidant biological processes. In 
      vitro treatment of Zn significantly increased Nrf2 and Sirt3 expression at gene 
      level and protein level respectively. Zn supplementation inhibited TPEN-induced 
      failure of cell survival and reversed the reduction of Nrf2 and Sirt3 expression, 
      which further reduced the production of ROS. Zn successfully presented its 
      anti-oxidation effect by activating Nrf2/Sirt3 signaling pathway in HUC-MSCs. Zn 
      supplementation may improve the efficacy of HUC-MSCs therapy with reduced 
      oxidative stress.
CI  - (c) The Author(s) 2021.
FAU - Lu, Xiaodan
AU  - Lu X
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
AD  - School of Medicine, Changchun University of Chinese Medicine, Changchun 130021, 
      China.
FAU - Lin, Yifan
AU  - Lin Y
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
AD  - School of Medicine, Changchun University of Chinese Medicine, Changchun 130021, 
      China.
FAU - Lin, Xiuying
AU  - Lin X
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
FAU - Zhang, Qiang
AU  - Zhang Q
AD  - Department of Clinical Laboratory, the Second Hospital of Jilin University, 
      Changchun 130041, China.
FAU - Wang, Zihang
AU  - Wang Z
AD  - General Surgery, the First Hospital of Jilin University, Changchun 130021, China.
FAU - Mi, Xuguang
AU  - Mi X
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
FAU - Wang, Ruobing
AU  - Wang R
AD  - General Surgery, the First Hospital of Jilin University, Changchun 130021, China.
FAU - Zhang, Xiaofang
AU  - Zhang X
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
AD  - School of Medicine, Changchun University of Chinese Medicine, Changchun 130021, 
      China.
FAU - Luan, Xu
AU  - Luan X
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
AD  - School of Medicine, Changchun University of Chinese Medicine, Changchun 130021, 
      China.
FAU - Liu, Yan
AU  - Liu Y
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
AD  - School of Medicine, Changchun University of Chinese Medicine, Changchun 130021, 
      China.
FAU - Li, Bing
AU  - Li B
AD  - Department of Clinical Laboratory, the Second Hospital of Jilin University, 
      Changchun 130041, China.
FAU - Tan, Yan
AU  - Tan Y
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
FAU - Fang, Yanqiu
AU  - Fang Y
AD  - Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, 
      China.
AD  - School of Medicine, Changchun University of Chinese Medicine, Changchun 130021, 
      China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Biophys Rep
JT  - Biophysics reports
JID - 101676603
PMC - PMC10235909
OTO - NOTNLM
OT  - Human umbilical cord mesenchymal stem cells
OT  - Nuclear factor erythroid-2-related factor 2
OT  - Oxidative stress
OT  - Sirtuin 3
OT  - Zinc
COIS- Xiaodan Lu, Yifan Lin, Xiuying Lin, Qiang Zhang, Zihang Wang, Xuguang Mi, Ruobing 
      Wang, Xiaofang Zhang, Xu Luan, Yan Liu, Bing Li, Yan Tan and Yanqiu Fang declare 
      that they have no conflict of interest.
EDAT- 2021/04/30 00:00
MHDA- 2021/04/30 00:01
PMCR- 2021/04/30
CRDT- 2023/06/08 04:30
PHST- 2020/09/11 00:00 [received]
PHST- 2021/03/05 00:00 [accepted]
PHST- 2021/04/30 00:01 [medline]
PHST- 2021/04/30 00:00 [pubmed]
PHST- 2023/06/08 04:30 [entrez]
PHST- 2021/04/30 00:00 [pmc-release]
AID - br-7-2-142 [pii]
AID - 10.52601/bpr.2021.200046 [doi]
PST - ppublish
SO  - Biophys Rep. 2021 Apr 30;7(2):142-151. doi: 10.52601/bpr.2021.200046.