PMID- 37293445
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230612
IS  - 2500-0462 (Print)
IS  - 2500-3259 (Electronic)
IS  - 2500-3259 (Linking)
VI  - 27
IP  - 3
DP  - 2023 Jun
TI  - Species identification of spider mites (Tetranychidae: Tetranychinae): a review 
      of methods.
PG  - 240-249
LID - 10.18699/VJGB-23-30 [doi]
AB  - Spider mites (Acari: Tetranychidae) are dangerous pests of agricultural and 
      ornamental crops, the most economically significant of them belonging to the 
      genera Tetranychus, Eutetranychus, Oligonychus and Panonychus. The expansion of 
      the distribution areas, the increased harmfulness and dangerous status of certain 
      species in the family Tetranychidae and their invasion of new regions pose a 
      serious threat to the phytosanitary status of agro- and biocenoses. Various 
      approaches to acarofauna species diagnosis determine a rather diverse range of 
      currently existing methods generally described in this review. Identification of 
      spider mites by morphological traits, which is currently considered the main 
      method, is complicated due to the complexity of preparing biomaterials for 
      diagnosis and a limited number of diagnostic signs. In this regard, biochemical 
      and molecular genetic methods such as allozyme analysis, DNA barcoding, 
      restriction fragment length polymorphism (PCR-RFLP), selection of 
      species-specific primers and real-time PCR are becoming important. In the review, 
      close attention is paid to the successful use of these methods for species 
      discrimination in the mites of the subfamily Tetranychinae. For some species, e. 
      g., the two-spotted spider mite (Tetranychus urticae), a range of identification 
      methods has been developed - from allozyme analysis to loop isothermal 
      amplification (LAMP), while for many other species a much smaller variety of 
      approaches is available. The greatest accuracy in the identification of spider 
      mites can be achieved using a combination of several methods, e. g., examination 
      of morphological features and one of the molecular approaches (DNA barcoding, 
      PCR-RFLP, etc.). This review may be useful to specialists who are in search of an 
      effective system for spider mite species identification as well as when 
      developing new test systems relevant to specific plant crops or a specific 
      region.
CI  - Copyright (c) AUTHORS.
FAU - Razuvaeva, A V
AU  - Razuvaeva AV
AD  - Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy 
      of Sciences, Novosibirsk, Russia.
FAU - Ulyanova, E G
AU  - Ulyanova EG
AD  - Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy 
      of Sciences, Krasnoobsk, Novosibirsk Region, Russia.
FAU - Skolotneva, E S
AU  - Skolotneva ES
AD  - Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy 
      of Sciences, Novosibirsk, Russia.
FAU - Andreeva, I V
AU  - Andreeva IV
AD  - Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy 
      of Sciences, Krasnoobsk, Novosibirsk Region, Russia.
LA  - eng
PT  - Journal Article
PL  - Russia (Federation)
TA  - Vavilovskii Zhurnal Genet Selektsii
JT  - Vavilovskii zhurnal genetiki i selektsii
JID - 101583566
PMC - PMC10244583
OTO - NOTNLM
OT  - COI
OT  - DNA barcoding
OT  - ITS
OT  - MALDI-TOF MS
OT  - PCR-RFLP
OT  - allozyme analysis
OT  - species identification
OT  - spider mites
COIS- The authors declare no conflict of interest.
EDAT- 2023/06/09 06:42
MHDA- 2023/06/09 06:43
PMCR- 2023/06/01
CRDT- 2023/06/09 04:36
PHST- 2022/07/14 00:00 [received]
PHST- 2022/12/25 00:00 [revised]
PHST- 2022/01/07 00:00 [accepted]
PHST- 2023/06/09 06:43 [medline]
PHST- 2023/06/09 06:42 [pubmed]
PHST- 2023/06/09 04:36 [entrez]
PHST- 2023/06/01 00:00 [pmc-release]
AID - 10.18699/VJGB-23-30 [doi]
PST - ppublish
SO  - Vavilovskii Zhurnal Genet Selektsii. 2023 Jun;27(3):240-249. doi: 
      10.18699/VJGB-23-30.