PMID- 37296219
OWN - NLM
STAT- MEDLINE
DCOM- 20230705
LR  - 20230705
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 370
DP  - 2023 Jan 17
TI  - Function of a hydrophobin in growth and development, nitrogen regulation, and 
      abiotic stress resistance of Ganoderma lucidum.
LID - fnad051 [pii]
LID - 10.1093/femsle/fnad051 [doi]
AB  - Fungal hydrophobins have many important physiological functions, such as 
      maintaining hydrophobicity and affecting virulence, growth, and development. In 
      Ganoderma lucidum, the molecular regulation mechanisms of hydrophobins in 
      mushroom are unclear. In this study, we investigated a hydrophobin protein 1 
      (Hyd1) in G. lucidum, which belongs to the fungal Class I hydrophobins. The hyd1 
      gene was highly expressed during the formation of primordia, and expression was 
      the lowest in fruiting bodies. Through the construction of hyd1 silenced strains, 
      we found that primordia formation was not initiated in these strains. This 
      finding indicated that Hyd1 played an important role in the development of G. 
      lucidum. Second, AreA, a key transcription factor in nitrogen metabolism, 
      negatively regulated the expression of hyd1. In an areA-silenced strain, the 
      expression of hyd1 increased by  approximately 14-fold compared with that of the wild-type (WT) 
      strain. Electrophoretic mobility shift assays (EMSA) indicated binding of AreA to 
      the promoter of hyd1. Additionally, expression of hyd1 was determined in the 
      presence of different nitrogen sources. Compared with that in the ammonia 
      nitrogen source, the expression of hyd1 in nitrate nitrogen source significantly 
      increased. Finally, we found that hyd1 plays important roles not only in nitrogen 
      regulation but also in the resistance to other abiotic stresses. After silencing 
      of hyd1, the resistance to heat, cell wall, and salt stresses decreased. Our 
      findings reveal the important roles of Hyd1 in the development and resistance to 
      abiotic stresses in G. lucidum and provide insights into the nitrogen regulation 
      mechanism of hydrophobins in higher basidiomycetes.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.
FAU - Qiao, Jinjin
AU  - Qiao J
AD  - Department of Microbiology, College of Life Sciences, Nanjing Agricultural 
      University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
FAU - Liu, He
AU  - Liu H
AD  - Department of Microbiology, College of Life Sciences, Nanjing Agricultural 
      University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
FAU - Xue, Peng
AU  - Xue P
AUID- ORCID: 0000-0002-8198-9222
AD  - Department of Microbiology, College of Life Sciences, Nanjing Agricultural 
      University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
FAU - Hong, Mengting
AU  - Hong M
AD  - Department of Microbiology, College of Life Sciences, Nanjing Agricultural 
      University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
FAU - Guo, Xiaoyu
AU  - Guo X
AD  - Department of Microbiology, College of Life Sciences, Nanjing Agricultural 
      University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
FAU - Xing, Zhenzhen
AU  - Xing Z
AD  - Department of Microbiology, College of Life Sciences, Nanjing Agricultural 
      University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
FAU - Zhao, Mingwen
AU  - Zhao M
AUID- ORCID: 0000-0002-9413-1743
AD  - Department of Microbiology, College of Life Sciences, Nanjing Agricultural 
      University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
FAU - Zhu, Jing
AU  - Zhu J
AUID- ORCID: 0000-0003-0694-7812
AD  - Department of Microbiology, College of Life Sciences, Nanjing Agricultural 
      University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Fungal Proteins)
SB  - IM
MH  - *Reishi
MH  - Fungal Proteins/metabolism
MH  - Stress, Physiological
MH  - Promoter Regions, Genetic
MH  - Growth and Development
OTO - NOTNLM
OT  - Ganoderma lucidum
OT  - abiotic stresses
OT  - growth and development
OT  - hydrophobin
OT  - nitrogen regulation
EDAT- 2023/06/10 15:14
MHDA- 2023/07/05 06:42
CRDT- 2023/06/09 23:23
PHST- 2022/12/28 00:00 [received]
PHST- 2023/05/23 00:00 [revised]
PHST- 2023/06/05 00:00 [accepted]
PHST- 2023/07/05 06:42 [medline]
PHST- 2023/06/10 15:14 [pubmed]
PHST- 2023/06/09 23:23 [entrez]
AID - 7193373 [pii]
AID - 10.1093/femsle/fnad051 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2023 Jan 17;370:fnad051. doi: 10.1093/femsle/fnad051.