PMID- 37300939
OWN - NLM
STAT- MEDLINE
DCOM- 20230804
LR  - 20230804
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 139
DP  - 2023 Aug
TI  - Association between proNGF receptors and apoptotic factors in human placentae.
PG  - 43-48
LID - S0143-4004(23)00118-2 [pii]
LID - 10.1016/j.placenta.2023.05.011 [doi]
AB  - INTRODUCTION: Earlier studies have shown higher apoptosis in the pre-term 
      placenta as compared to term. However, the exact mechanisms triggering these are 
      not completely understood. Studies in neuronal and non-neuronal tissues have 
      shown that the precursor form of NGF (proNGF) triggers apoptosis through 
      preferential activation of p75NTR and sortilin receptors. We therefore, 
      investigated placental expression of proNGF, mature NGF, p75NTR, co-receptor 
      sortilin and their association with apoptosis. We further compared the levels of 
      pro-protein convertase, furin between samples having high and low proNGF: mature 
      NGF ratio. METHODS: Placenta samples were collected from women delivering at term 
      (>/=37 weeks; n = 41) and preterm (<37 weeks; n = 44). The protein levels of NGF, 
      proNGF, p75NTR, Bax, Bcl-2 and furin were estimated by ELISA. Mean values of 
      variables between different groups were compared using the independent sample 
      t-test and associations were studied by Pearson correlation analysis. RESULTS: 
      The placental mature NGF, proNGF and p75NTR protein levels were comparable 
      between groups. Bax: Bcl-2 ratio was higher in preterm (p < 0.05) compared to 
      term placenta. p75NTR was positively associated with Bax levels and sortilin 
      levels were positively associated with p75NTR in whole cohort as well as 
      individual groups. DISCUSSION: The higher Bax: Bcl-2 ratio in preterm placenta 
      suggests the sensitivity to apoptosis. There were no differences in levels of 
      NGF, proNGF, p75NTR, sortilin, and furin between groups. The observed 
      associations between p75NTR, sortilin and Bax suggest that p75NTR and sortilin 
      mediated signalling may be involved in the mechanisms leading to higher apoptosis 
      in preterm placentae.
CI  - Copyright (c) 2023. Published by Elsevier Ltd.
FAU - Pathare-Ingawale, Prachi
AU  - Pathare-Ingawale P
AD  - Interdisciplinary School of Health Sciences, Savitribai Phule Pune University, 
      Pune, India.
FAU - Gogate, Niharika
AU  - Gogate N
AD  - Interdisciplinary School of Health Sciences, Savitribai Phule Pune University, 
      Pune, India.
FAU - Thube, Yogesh
AU  - Thube Y
AD  - Interdisciplinary School of Health Sciences, Savitribai Phule Pune University, 
      Pune, India.
FAU - Mansour, Salma M A
AU  - Mansour SMA
AD  - Interdisciplinary School of Health Sciences, Savitribai Phule Pune University, 
      Pune, India.
FAU - Chavan-Gautam, Preeti
AU  - Chavan-Gautam P
AD  - Interdisciplinary School of Health Sciences, Savitribai Phule Pune University, 
      Pune, India. Electronic address: chavanpriti@gmail.com.
FAU - Wagh, Girija
AU  - Wagh G
AD  - Department of Obstetrics and Gynecology, Bharati Medical College and Hospital, 
      Bharati Vidyapeeth University, Pune, India.
FAU - Joshi, Sadhana
AU  - Joshi S
AD  - Interactive Research School for Health Affairs, Bharati Vidyapeeth University, 
      Pune, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230601
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - EC 3.4.21.75 (Furin)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (Carrier Proteins)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Pregnancy
MH  - Infant, Newborn
MH  - Humans
MH  - Female
MH  - *Furin/metabolism
MH  - bcl-2-Associated X Protein
MH  - *Placenta/metabolism
MH  - Carrier Proteins
MH  - Nerve Growth Factor/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Bax
OT  - Bcl-2
OT  - Furin
OT  - Neurotrophins
OT  - Placenta
OT  - Pro-neurotrophins
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this article.
EDAT- 2023/06/11 01:06
MHDA- 2023/08/04 06:43
CRDT- 2023/06/10 18:01
PHST- 2022/11/03 00:00 [received]
PHST- 2023/04/26 00:00 [revised]
PHST- 2023/05/17 00:00 [accepted]
PHST- 2023/08/04 06:43 [medline]
PHST- 2023/06/11 01:06 [pubmed]
PHST- 2023/06/10 18:01 [entrez]
AID - S0143-4004(23)00118-2 [pii]
AID - 10.1016/j.placenta.2023.05.011 [doi]
PST - ppublish
SO  - Placenta. 2023 Aug;139:43-48. doi: 10.1016/j.placenta.2023.05.011. Epub 2023 Jun 
      1.