PMID- 37301124 OWN - NLM STAT- MEDLINE DCOM- 20230726 LR - 20230726 IS - 1878-1705 (Electronic) IS - 1567-5769 (Linking) VI - 121 DP - 2023 Aug TI - Effects of a lycopene-layered double hydroxide composite administration in cells and lungs of adult mice. PG - 110454 LID - S1567-5769(23)00777-4 [pii] LID - 10.1016/j.intimp.2023.110454 [doi] AB - Lycopene is a natural compound with one of the highest antioxidant activities. Its consumption is associated with lower risks in lung cancer and chronic obstructive pulmonary disease, for example. Experimentally, a murine model demonstrated the ingestion of lycopene, which reduced the damage in lungs caused by cigarette smoke. Since lycopene is highly hydrophobic, its formulations in supplements and preparations for laboratory assays are based on oils, additionally, bioavailavility is low. We developed a lycopene layered double hydroxide (Lyc-LDH) composite, which is capable of transporting lycopene aqueous media. Our objective was to evaluate the cytotoxicity of Lyc-LDH and the intra-cellular production of reactive oxygen species (ROS) in J774A.1 cells. Also, in vivo assays were conducted with 50 male C57BL/6 mice intranasally treated with Lyc-LDH 10 mg/kg (LG10), Lyc-LDH 25 mg/kg (LG25) and Lyc-LDH 50 mg/kg (LG50) during five days compared against a vehicle (VG) and control (CG) group. The blood, bronchoalveolar lavage fluid (BALF) and lung tissue were analyzed. The results revealed that Lyc-LDH composite attenuated intracellular ROS production stimulated with lipopolysacharide. In BALF, the highest doses of Lyc-LDH (LG25 and LG50) promoted influx of macrophages, lymphocytes, neutrophils and eosinophils compared to CG and VG. Also, LG50 increased the levels of IL-6 and IL-13, and promoted the redox imbalance in the pulmonary tissue. On the contrary, low concentrations did not produce significative effects. In conclusion, our results suggest that intranasal administration of high concentrations of Lyc-LDH induces inflammation as well as redox status changes in the lungs of healthy mice, however, results with low concentrations open a promising way to study LDH composites as vehicles for intranasal administration of antioxidant coadjuvants. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - de Carvalho, Iriane Marques AU - de Carvalho IM AD - Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences and Center of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - de Souza, Ana Beatriz Farias AU - de Souza ABF AD - Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences and Center of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Castro, Thalles de Feitas AU - Castro TF AD - Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences and Center of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Machado-Junior, Pedro Alves AU - Machado-Junior PA AD - Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences and Center of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Menezes, Tatiana Prata AU - Menezes TP AD - Laboratory of Immunobiology of Inflammation (LABIIN), Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Dias, Andreia da Silva AU - Dias ADS AD - Laboratory of Neurobiology and Biomaterials (LNBio), Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Oliveira, Laser Antonio Machado AU - Oliveira LAM AD - Laboratory of Neurobiology and Biomaterials (LNBio), Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Nogueira, Katiane de Oliveira Pinto Coelho AU - Nogueira KOPC AD - Laboratory of Neurobiology and Biomaterials (LNBio), Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Talvani, Andre AU - Talvani A AD - Laboratory of Immunobiology of Inflammation (LABIIN), Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Cangussu, Silvia Dantas AU - Cangussu SD AD - Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences and Center of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. FAU - Arizaga, Gregorio Guadalupe Carbajal AU - Arizaga GGC AD - Chemistry Department, University of Guadalajara, 44430 Guadalajara, Jalisco, Mexico. FAU - Bezerra, Frank Silva AU - Bezerra FS AD - Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences and Center of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000 Ouro Preto, Minas Gerais, Brazil. Electronic address: frank@ufop.edu.br. LA - eng PT - Journal Article DEP - 20230608 PL - Netherlands TA - Int Immunopharmacol JT - International immunopharmacology JID - 100965259 RN - SB0N2N0WV6 (Lycopene) RN - 0 (Antioxidants) RN - 0 (Reactive Oxygen Species) RN - 0 (Hydroxides) SB - IM MH - Mice MH - Male MH - Animals MH - Lycopene/pharmacology MH - *Antioxidants/pharmacology MH - Reactive Oxygen Species MH - *Oxidative Stress MH - Mice, Inbred C57BL MH - Lung/metabolism MH - Hydroxides/pharmacology OTO - NOTNLM OT - Inflammation OT - Intranasal OT - Layered double hydroxide OT - Lung OT - Lycopene OT - Oxidative stress COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/06/11 01:06 MHDA- 2023/07/26 06:43 CRDT- 2023/06/10 18:09 PHST- 2023/03/14 00:00 [received] PHST- 2023/05/24 00:00 [revised] PHST- 2023/06/02 00:00 [accepted] PHST- 2023/07/26 06:43 [medline] PHST- 2023/06/11 01:06 [pubmed] PHST- 2023/06/10 18:09 [entrez] AID - S1567-5769(23)00777-4 [pii] AID - 10.1016/j.intimp.2023.110454 [doi] PST - ppublish SO - Int Immunopharmacol. 2023 Aug;121:110454. doi: 10.1016/j.intimp.2023.110454. Epub 2023 Jun 8.