PMID- 37302070
OWN - NLM
STAT- Publisher
LR  - 20230620
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 4
IP  - 2
DP  - 2023 Jun 9
TI  - Protocol for the use of signal amplification by exchange reaction-fluorescence in 
      situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissue.
PG  - 102353
LID - S2666-1667(23)00320-9 [pii]
LID - 10.1016/j.xpro.2023.102353 [doi]
LID - 102353
AB  - Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA 
      expression, but difficulties arise with low-abundance RNA and in tissues that are 
      formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive. In 
      this protocol, we adapt a previously designed FISH amplification protocol (SABER 
      [signal amplification by exchange reaction]) for adult mouse FFPE lung sections 
      by using probes that are extended and branched to amplify the signal. We combine 
      FISH and immunostaining to identify cell-specific RNA. For complete details on 
      the use and execution of this protocol, please refer to Kishi et al.(1) and Lyu 
      et al.(2).
CI  - Copyright (c) 2023. Published by Elsevier Inc.
FAU - Warren, Rachel
AU  - Warren R
AD  - Department of Medicine, Mayo Clinic, Rochester, MN 55902, USA. Electronic 
      address: warren.rachel@mayo.edu.
FAU - Shaik, Aftab
AU  - Shaik A
AD  - Department of Medicine, Mayo Clinic, Rochester, MN 55902, USA.
FAU - Teubner, Lauren
AU  - Teubner L
AD  - Department of Medicine, Mayo Clinic, Rochester, MN 55902, USA.
FAU - Lyu, Handeng
AU  - Lyu H
AD  - Department of Medicine, Mayo Clinic, Rochester, MN 55902, USA.
FAU - De Langhe, Stijn
AU  - De Langhe S
AD  - Department of Medicine, Mayo Clinic, Rochester, MN 55902, USA. Electronic 
      address: delanghe.stijn@mayo.edu.
LA  - eng
GR  - R35 HL161169/HL/NHLBI NIH HHS/United States
GR  - T32 HL105355/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20230609
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
SB  - IM
PMC - PMC10276273
OTO - NOTNLM
OT  - Gene Expression
OT  - In Situ Hybridization
OT  - Microscopy
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2023/06/11 19:15
MHDA- 2023/06/11 19:15
PMCR- 2023/06/09
CRDT- 2023/06/11 12:36
PHST- 2023/02/24 00:00 [received]
PHST- 2023/04/11 00:00 [revised]
PHST- 2023/05/12 00:00 [accepted]
PHST- 2023/06/11 19:15 [medline]
PHST- 2023/06/11 19:15 [pubmed]
PHST- 2023/06/11 12:36 [entrez]
PHST- 2023/06/09 00:00 [pmc-release]
AID - S2666-1667(23)00320-9 [pii]
AID - 102353 [pii]
AID - 10.1016/j.xpro.2023.102353 [doi]
PST - aheadofprint
SO  - STAR Protoc. 2023 Jun 9;4(2):102353. doi: 10.1016/j.xpro.2023.102353.