PMID- 37302847
OWN - NLM
STAT- MEDLINE
DCOM- 20230804
LR  - 20230901
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 85
IP  - 8
DP  - 2023 Aug 1
TI  - Evaluation of the influence of the C-X-C motif chemokine ligand 12 / C-X-C 
      chemokine receptor 4 axis on canine mammary gland tumor cell migration.
PG  - 837-843
LID - 10.1292/jvms.23-0126 [doi]
AB  - C-X-C motif chemokine ligand 12 (CXCL12) is one of the chemokines that binds to 
      C-X-C chemokine receptor 4 (CXCR4) on tumor cell membranes and induces chemotaxis 
      and/or migration. Mammary gland tumors (MGT) are the most common neoplasms in 
      intact female dogs, with local invasion and distant metastasis regarded as 
      problems. However, the influence of the CXCL12/CXCR4 axis on canine MGT cell 
      migration has not been elucidated. This study aimed to evaluate the expression of 
      CXCL12 and CXCR4 in canine MGT cells and tissues and investigate the influence of 
      CXCL12 protein on the migratory ability of MGT cells. CXCL12 expression was 
      evaluated in 10 canine malignant MGT tissues. CXCL12 expression in tumor cells 
      was identified in all examined tissues; however, the staining pattern and 
      intensity differed between the tumors. Immunocytochemistry revealed three canine 
      MGT cell lines as CXCR4-positive. Migratory ability was evaluated using a wound 
      healing assay, and the migration of CXCR4-positive MGT cells was significantly 
      activated by the addition of CXCL12 protein. This influence was canceled by 
      pre-treatment with a CXCR4 antagonist. The results of our study suggest that the 
      CXCL12/CXCR4 axis may be associated with the migration of canine MGT.
FAU - Kudo, Ayano
AU  - Kudo A
AD  - Laboratory of Small Animal Surgery, School of Veterinary Medicine, Azabu 
      University, Kanagawa, Japan.
FAU - Sawahata, Hiroki
AU  - Sawahata H
AD  - Laboratory of Small Animal Surgery, School of Veterinary Medicine, Azabu 
      University, Kanagawa, Japan.
FAU - Yoshimoto, Sho
AU  - Yoshimoto S
AD  - Laboratory of Small Animal Surgery, School of Veterinary Medicine, Azabu 
      University, Kanagawa, Japan.
AD  - Department of Clinical Sciences and Advanced Medicine, School of Veterinary 
      Medicine, University of Pennsylvania, Philadelphia, PA, USA.
FAU - Yamauchi, Akinori
AU  - Yamauchi A
AD  - Laboratory of Small Animal Surgery, School of Veterinary Medicine, Azabu 
      University, Kanagawa, Japan.
FAU - Oshita, Ryo
AU  - Oshita R
AD  - Laboratory of Small Animal Surgery, School of Veterinary Medicine, Azabu 
      University, Kanagawa, Japan.
FAU - Kanai, Eiichi
AU  - Kanai E
AD  - Laboratory of Small Animal Surgery, School of Veterinary Medicine, Azabu 
      University, Kanagawa, Japan.
AD  - Azabu University Veterinary Teaching Hospital, School of Veterinary Medicine, 
      Azabu University, Kanagawa, Japan.
FAU - Takagi, Satoshi
AU  - Takagi S
AD  - Laboratory of Small Animal Surgery, School of Veterinary Medicine, Azabu 
      University, Kanagawa, Japan.
AD  - Azabu University Veterinary Teaching Hospital, School of Veterinary Medicine, 
      Azabu University, Kanagawa, Japan.
LA  - eng
PT  - Journal Article
DEP - 20230612
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Ligands)
RN  - 0 (Chemokine CXCL12)
SB  - IM
MH  - Dogs
MH  - Animals
MH  - Female
MH  - *Receptors, CXCR4/metabolism
MH  - Ligands
MH  - *Chemokine CXCL12/metabolism
MH  - Cell Movement
MH  - Cell Line, Tumor
PMC - PMC10466059
OTO - NOTNLM
OT  - C-X-C motif chemokine ligand 12 / C-X-C chemokine receptor 4 axis
OT  - canine tumor
OT  - chemotaxis
OT  - mammary gland tumor
OT  - migration assay
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2023/06/12 00:42
MHDA- 2023/08/04 06:43
PMCR- 2023/08/01
CRDT- 2023/06/11 21:23
PHST- 2023/08/04 06:43 [medline]
PHST- 2023/06/12 00:42 [pubmed]
PHST- 2023/06/11 21:23 [entrez]
PHST- 2023/08/01 00:00 [pmc-release]
AID - 23-0126 [pii]
AID - 10.1292/jvms.23-0126 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2023 Aug 1;85(8):837-843. doi: 10.1292/jvms.23-0126. Epub 2023 Jun 
      12.