PMID- 37303798 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230919 IS - 1664-302X (Print) IS - 1664-302X (Electronic) IS - 1664-302X (Linking) VI - 14 DP - 2023 TI - Cassava begomovirus species diversity changes during plant vegetative cycles. PG - 1163566 LID - 10.3389/fmicb.2023.1163566 [doi] LID - 1163566 AB - Cassava is a root crop important for global food security and the third biggest source of calories on the African continent. Cassava production is threatened by Cassava mosaic disease (CMD), which is caused by a complex of single-stranded DNA viruses (family: Geminiviridae, genus: Begomovirus) that are transmitted by the sweet potato whitefly (Bemisia tabaci). Understanding the dynamics of different cassava mosaic begomovirus (CMB) species through time is important for contextualizing disease trends. Cassava plants with CMD symptoms were sampled in Lake Victoria and coastal regions of Kenya before transfer to a greenhouse setting and regular propagation. The field-collected and greenhouse samples were sequenced using Illumina short-read sequencing and analyzed on the Galaxy platform. In the field-collected samples, African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), East African cassava mosaic Kenya virus (EACMKV), and East African cassava mosaic virus-Uganda variant (EACMV-Ug) were detected in samples from the Lake Victoria region, while EACMV and East African mosaic Zanzibar virus (EACMZV) were found in the coastal region. Many of the field-collected samples had mixed infections of EACMV and another begomovirus. After 3 years of regrowth in the greenhouse, only EACMV-like viruses were detected in all samples. The results suggest that in these samples, EACMV becomes the dominant virus through vegetative propagation in a greenhouse. This differed from whitefly transmission results. Cassava plants were inoculated with ACMV and another EACMV-like virus, East African cassava mosaic Cameroon virus (EACMCV). Only ACMV was transmitted by whiteflies from these plants to recipient plants, as indicated by sequencing reads and copy number data. These results suggest that whitefly transmission and vegetative transmission lead to different outcomes for ACMV and EACMV-like viruses. CI - Copyright (c) 2023 Dye, Muga, Mwangi, Hoyer, Ly, Rosado, Sharpee, Mware, Wambugu, Labadie, Deppong, Jackai, Jacobson, Kennedy, Ateka, Duffy, Hanley-Bowdoin, Carbone and Ascencio-Ibanez. FAU - Dye, Anna E AU - Dye AE AD - Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States. FAU - Muga, Brenda AU - Muga B AD - Department of Horticulture, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya. FAU - Mwangi, Jenniffer AU - Mwangi J AD - Department of Horticulture, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya. FAU - Hoyer, J Steen AU - Hoyer JS AD - Department of Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, NJ, United States. FAU - Ly, Vanessa AU - Ly V AD - Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, United States. FAU - Rosado, Yamilex AU - Rosado Y AD - Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, United States. FAU - Sharpee, William AU - Sharpee W AD - International Livestock Research Institute (ILRI), Nairobi, Kenya. AD - Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. FAU - Mware, Benard AU - Mware B AD - International Livestock Research Institute (ILRI), Nairobi, Kenya. FAU - Wambugu, Mary AU - Wambugu M AD - International Livestock Research Institute (ILRI), Nairobi, Kenya. FAU - Labadie, Paul AU - Labadie P AD - Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. FAU - Deppong, David AU - Deppong D AD - Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States. FAU - Jackai, Louis AU - Jackai L AD - Department of Natural Resources and Environmental Design, North Carolina Agricultural and Technical State University, Greensboro, NC, United States. FAU - Jacobson, Alana AU - Jacobson A AD - Department of Entomology and Plant Pathology, Auburn University, Auburn, AL, United States. FAU - Kennedy, George AU - Kennedy G AD - Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. FAU - Ateka, Elijah AU - Ateka E AD - Department of Horticulture, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya. FAU - Duffy, Siobain AU - Duffy S AD - Department of Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, NJ, United States. FAU - Hanley-Bowdoin, Linda AU - Hanley-Bowdoin L AD - Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States. FAU - Carbone, Ignazio AU - Carbone I AD - Center for Integrated Fungal Research, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. FAU - Ascencio-Ibanez, Jose Trinidad AU - Ascencio-Ibanez JT AD - Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, United States. LA - eng PT - Journal Article DEP - 20230525 PL - Switzerland TA - Front Microbiol JT - Frontiers in microbiology JID - 101548977 PMC - PMC10248227 OTO - NOTNLM OT - Cassava (Manihot esculenta) OT - plant virus OT - vector transmission OT - vegetative (asexual) propagation OT - whitefly (Bemisia tabaci) COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2023/06/12 06:42 MHDA- 2023/06/12 06:43 PMCR- 2023/05/25 CRDT- 2023/06/12 03:58 PHST- 2023/02/10 00:00 [received] PHST- 2023/04/17 00:00 [accepted] PHST- 2023/06/12 06:43 [medline] PHST- 2023/06/12 06:42 [pubmed] PHST- 2023/06/12 03:58 [entrez] PHST- 2023/05/25 00:00 [pmc-release] AID - 10.3389/fmicb.2023.1163566 [doi] PST - epublish SO - Front Microbiol. 2023 May 25;14:1163566. doi: 10.3389/fmicb.2023.1163566. eCollection 2023.