PMID- 37304779
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230613
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 13
IP  - 25
DP  - 2023 Jun 5
TI  - Improved stability and activity of laccase through de novo and post-synthesis 
      immobilization on a hierarchically porous metal-organic framework (ZIF-8).
PG  - 17194-17201
LID - 10.1039/d3ra01571h [doi]
AB  - Porous materials such as metal-organic frameworks (MOFs) are considered to be 
      suitable materials for immobilizing enzymes to improve their stability. However, 
      conventional MOFs reduce the enzymes' catalytic activity due to difficulties with 
      mass transfer and diffusing reactants after their micropores are occupied by 
      enzyme molecules. To address these issues, a novel hierarchically structured 
      zeolitic imidazolate framework-8 (HZIF-8) was prepared to study the effects of 
      different laccase immobilization approaches such as the post-synthesis 
      (LAC@HZIF-8-P) and de novo (LAC@HZIF-8-D) immobilization of catalytic activities 
      for removing 2,4-dichlorophenol (2,4-DCP). The results showed higher catalytic 
      activity for the laccase-immobilized LAC@HZIF-8 prepared using different methods 
      than for the LAC@MZIF-8 sample, with 80% of 2,4-DCP removed under optimal 
      conditions. These results could be attributable to the multistage structure of 
      HZIF-8. The LAC@HZIF-8-D sample was stable and superior to LAC@HZIF-8-P, 
      maintaining a 2,4-DCP removal efficiency of 80% after three recycles and 
      demonstrating superior laccase thermostability and storage stability. Moreover, 
      after loading with copper nanoparticles, the LAC@HZIF-8-D approach exhibited a 
      2,4-DCP removal efficiency of 95%, a promising finding for its potential use in 
      environmental purification.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Xu, Ran
AU  - Xu R
AD  - College of Environmental Science and Engineering, State Key Laboratory of 
      Pollution Control and Resource Reuse, Tongji University 1239 Siping Rd. Shanghai 
      200092 China 51n@tongji.edu.cn.
AD  - Shanghai Institute of Pollution Control and Ecological Security 1239 Siping Rd. 
      Shanghai 200092 China.
FAU - Zhang, Xujie
AU  - Zhang X
AD  - College of Environmental Science and Engineering, State Key Laboratory of 
      Pollution Control and Resource Reuse, Tongji University 1239 Siping Rd. Shanghai 
      200092 China 51n@tongji.edu.cn.
AD  - Shanghai Institute of Pollution Control and Ecological Security 1239 Siping Rd. 
      Shanghai 200092 China.
FAU - Zelekew, Osman Ahmend
AU  - Zelekew OA
AUID- ORCID: 0000-0003-2633-5426
AD  - College of Environmental Science and Engineering, State Key Laboratory of 
      Pollution Control and Resource Reuse, Tongji University 1239 Siping Rd. Shanghai 
      200092 China 51n@tongji.edu.cn.
AD  - Shanghai Institute of Pollution Control and Ecological Security 1239 Siping Rd. 
      Shanghai 200092 China.
AD  - Department of Materials Science and Engineering, Adama Science and Technology 
      University Adama Ethiopia.
FAU - Schott, Eduardo
AU  - Schott E
AD  - Department of Inorganic Chemistry of the Faculty of Chemistry and Pharmacy, 
      Pontificia Universidad Catolica de Chile Vicuna Mackenna 4860, Macul Santiago 
      Chile edschott@uc.cl.
FAU - Wu, Yi-Nan
AU  - Wu YN
AUID- ORCID: 0000-0001-6703-019X
AD  - College of Environmental Science and Engineering, State Key Laboratory of 
      Pollution Control and Resource Reuse, Tongji University 1239 Siping Rd. Shanghai 
      200092 China 51n@tongji.edu.cn.
AD  - Shanghai Institute of Pollution Control and Ecological Security 1239 Siping Rd. 
      Shanghai 200092 China.
LA  - eng
PT  - Journal Article
DEP - 20230608
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC10248541
COIS- There are no conflicts to declare.
EDAT- 2023/06/12 06:42
MHDA- 2023/06/12 06:43
PMCR- 2023/06/08
CRDT- 2023/06/12 04:14
PHST- 2023/03/10 00:00 [received]
PHST- 2023/05/26 00:00 [accepted]
PHST- 2023/06/12 06:43 [medline]
PHST- 2023/06/12 06:42 [pubmed]
PHST- 2023/06/12 04:14 [entrez]
PHST- 2023/06/08 00:00 [pmc-release]
AID - d3ra01571h [pii]
AID - 10.1039/d3ra01571h [doi]
PST - epublish
SO  - RSC Adv. 2023 Jun 8;13(25):17194-17201. doi: 10.1039/d3ra01571h. eCollection 2023 
      Jun 5.