PMID- 37305810
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231127
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 17
IP  - 1
DP  - 2023
TI  - Comparative karyotype analysis of eight Cucurbitaceae crops using fluorochrome 
      banding and 45S rDNA-FISH.
PG  - 31-58
LID - 10.3897/compcytogen.v17.i1.99236 [doi]
AB  - To have an insight into the karyotype variation of eight Cucurbitaceae crops 
      including Cucumissativus Linnaeus, 1753, Cucumismelo Linnaeus, 1753, 
      Citrulluslanatus (Thunberg, 1794) Matsumura et Nakai, 1916, Benincasahispida 
      (Thunberg, 1784) Cogniaux, 1881, Momordicacharantia Linnaeus, 1753, 
      Luffacylindrica (Linnaeus, 1753) Roemer, 1846, Lagenariasicerariavar.hispida 
      (Thunberg, 1783) Hara, 1948 and Cucurbitamoschata Duchesne ex Poiret, 1819, well 
      morphologically differentiated mitotic metaphase chromosomes were prepared using 
      the enzymatic maceration and flame-drying method, and the chromosomal 
      distribution of heterochromatin and 18S-5.8S-26S rRNA genes (45S rDNA) was 
      investigated using sequential combined PI and DAPI (CPD) staining and 
      fluorescence in situ hybridization (FISH) with 45S rDNA probe. Detailed 
      karyotypes were established using the dataset of chromosome measurements, 
      fluorochrome bands and rDNA FISH signals. Four karyotype asymmetry indices, 
      CV(CI), CV(CL), M(CA) and Stebbins' category, were measured to elucidate the 
      karyological relationships among species. All the species studied had symmetrical 
      karyotypes composed of metacentric and submetacentric or only metacentric 
      chromosomes, but their karyotype structure can be discriminated by the scatter 
      plot of M(CA) vs. CV(CL). The karyological relationships among these species 
      revealed by PCoA based on x, 2n, TCL, M(CA), CV(CL) and CV(CI) was basically in 
      agreement with the phylogenetic relationships revealed by DNA sequences. CPD 
      staining revealed all 45S rDNA sites in all species, (peri)centromeric GC-rich 
      heterochromatin in C.sativus, C.melo, C.lanatus, M.charantia and L.cylindrica, 
      terminal GC-rich heterochromatin in C.sativus. DAPI counterstaining after FISH 
      revealed pericentromeric DAPI(+) heterochromatin in C.moschata. rDNA FISH 
      detected two 45S loci in five species and five 45S loci in three species. Among 
      these 45S loci, most were located at the terminals of chromosome arms, and a few 
      in the proximal regions. In C.sativus, individual chromosomes can be precisely 
      distinguished by the CPD band and 45S rDNA signal patterns, providing an easy 
      method for chromosome identification of cucumber. The genome differentiation 
      among these species was discussed in terms of genome size, heterochromatin, 45S 
      rDNA site, and karyotype asymmetry based on the data of this study and previous 
      reports.
CI  - Chao-Wen She, Xiang-Hui Jiang, Chun-Ping He.
FAU - She, Chao-Wen
AU  - She CW
AUID- ORCID: 0000-0003-1935-5509
AD  - Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of 
      Hunan Province, Huaihua University, Huaihua, Hunan, 418008, China Huaihua 
      University Huaihua China.
AD  - College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou, 
      Hunan, 412007, China Hunan University of Technology Zhuzhou China.
FAU - Jiang, Xiang-Hui
AU  - Jiang XH
AUID- ORCID: 0000-0003-0923-210X
AD  - Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of 
      Hunan Province, Huaihua University, Huaihua, Hunan, 418008, China Huaihua 
      University Huaihua China.
FAU - He, Chun-Ping
AU  - He CP
AD  - College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou, 
      Hunan, 412007, China Hunan University of Technology Zhuzhou China.
LA  - eng
PT  - Journal Article
DEP - 20230209
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC10252140
OTO - NOTNLM
OT  - Cucurbitaceae
OT  - cytotaxonomy
OT  - fluorescence in situ hybridization
OT  - fluorochrome banding
OT  - karyotype
OT  - karyotype asymmetry
OT  - ribosomal RNA genes (rDNA)
COIS- The authors have declared that no competing interests exist.
EDAT- 2023/06/12 06:42
MHDA- 2023/06/12 06:43
PMCR- 2023/02/09
CRDT- 2023/06/12 04:28
PHST- 2022/12/24 00:00 [received]
PHST- 2023/01/23 00:00 [accepted]
PHST- 2023/06/12 06:43 [medline]
PHST- 2023/06/12 06:42 [pubmed]
PHST- 2023/06/12 04:28 [entrez]
PHST- 2023/02/09 00:00 [pmc-release]
AID - 99236 [pii]
AID - 10.3897/compcytogen.v17.i1.99236 [doi]
PST - epublish
SO  - Comp Cytogenet. 2023 Feb 9;17(1):31-58. doi: 10.3897/compcytogen.v17.i1.99236. 
      eCollection 2023.