PMID- 37306239
OWN - NLM
STAT- MEDLINE
DCOM- 20230719
LR  - 20240315
IS  - 1753-4267 (Electronic)
IS  - 1753-4259 (Print)
IS  - 1753-4259 (Linking)
VI  - 29
IP  - 5
DP  - 2023 Jul
TI  - Single cell RNA-sequencing of human precision-cut lung slices: A novel approach 
      to study the effect of vaping and viral infection on lung health.
PG  - 61-70
LID - 10.1177/17534259231181029 [doi]
AB  - Vaping is an increasing health threat in the US and worldwide. The damaging 
      impact of vaping on the human distal lung has been highlighted by the recent 
      epidemic of electronic cigarette or vaping use-associated lung injury (EVALI). 
      The pathogenesis of EVALI remains incompletely understood, due to a paucity of 
      models that recapitulate the structural and functional complexity of the human 
      distal lung and the still poorly defined culprit exposures to vaping products and 
      respiratory viral infections. Our aim was to establish the feasibility of using 
      single cell RNA-sequencing (scRNA-seq) technology in human precision-cut lung 
      slices (PCLS) as a more physiologically relevant model to better understand how 
      vaping regulates the antiviral and pro-inflammatory response to influenza A virus 
      infection. Normal healthy donor PCLS were treated with vaping extract and 
      influenza A viruses for scRNA-seq analysis. Vaping extract augmented host 
      antiviral and pro-inflammatory responses in structural cells such as lung 
      epithelial cells and fibroblasts, as well as in immune cells such as macrophages 
      and monocytes. Our findings suggest that human distal lung slice model is useful 
      to study the heterogeneous responses of immune and structural cells under EVALI 
      conditions, such as vaping and respiratory viral infection.
FAU - Crue, Taylor
AU  - Crue T
AD  - Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, 
      CO, USA.
FAU - Lee, Grace Yihua
AU  - Lee GY
AD  - Singulomics Corporation, Bronx, NY, USA.
FAU - Peng, Joyce Yao-Chun
AU  - Peng JY
AD  - Singulomics Corporation, Bronx, NY, USA.
FAU - Schaunaman, Niccolette
AU  - Schaunaman N
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Agraval, Hina
AU  - Agraval H
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Day, Brian J
AU  - Day BJ
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Dimasuay, Kris Genelyn
AU  - Dimasuay KG
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Cervantes, Diana
AU  - Cervantes D
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Nouri, Hamid
AU  - Nouri H
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Nichols, Taylor
AU  - Nichols T
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Hartsoe, Paige
AU  - Hartsoe P
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Numata, Mari
AU  - Numata M
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Petrache, Irina
AU  - Petrache I
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
FAU - Chu, Hong Wei
AU  - Chu HW
AUID- ORCID: 0000-0002-8329-4803
AD  - Department of Medicine, National Jewish Health, Denver, CO, USA.
LA  - eng
GR  - U19 AI125357/AI/NIAID NIH HHS/United States
GR  - R01 HL077328/HL/NHLBI NIH HHS/United States
GR  - R01 AI150082/AI/NIAID NIH HHS/United States
GR  - R01 AI152504/AI/NIAID NIH HHS/United States
GR  - R01 AI161296/AI/NIAID NIH HHS/United States
GR  - R01 HL144396/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20230612
PL  - United States
TA  - Innate Immun
JT  - Innate immunity
JID - 101469670
RN  - 0 (Antiviral Agents)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Humans
MH  - *Lung Injury
MH  - *Vaping/adverse effects
MH  - *Electronic Nicotine Delivery Systems
MH  - Lung
MH  - *Virus Diseases
MH  - Antiviral Agents
MH  - RNA
PMC - PMC10357887
OTO - NOTNLM
OT  - electronic cigarettes
OT  - human
OT  - lung slices
OT  - single cell RNA-sequencing
OT  - vaping
OT  - viral infection
COIS- The author(s) declared no potential conflicts of interest with respect to the 
      research, authorship, and/or publication of this article.
EDAT- 2023/06/12 13:07
MHDA- 2023/07/19 06:42
PMCR- 2023/06/12
CRDT- 2023/06/12 07:13
PHST- 2023/07/19 06:42 [medline]
PHST- 2023/06/12 13:07 [pubmed]
PHST- 2023/06/12 07:13 [entrez]
PHST- 2023/06/12 00:00 [pmc-release]
AID - 10.1177_17534259231181029 [pii]
AID - 10.1177/17534259231181029 [doi]
PST - ppublish
SO  - Innate Immun. 2023 Jul;29(5):61-70. doi: 10.1177/17534259231181029. Epub 2023 Jun 
      12.