PMID- 37308409
OWN - NLM
STAT- MEDLINE
DCOM- 20230614
LR  - 20230614
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 38
IP  - 6
DP  - 2022 Nov
TI  - [Intervention effects of estradiol on myocardial ischemia- reperfusion injury of 
      rat and its mechanisms].
PG  - 638-643
LID - 10.12047/j.cjap.6362.2022.116 [doi]
AB  - Objective: To study the effects of estradiol (E2) on alleviating myocardial 
      ischemia/reperfusion(I/R) injury through estrogen receptorbeta(ERbeta) mediated 
      extracellular regulated protein kinases(ERK) pathway activation. Methods: 
      Eighty-four adult female SD rats were ovariectomized and randomly divided into 
      control group, NC siRNA adeno-associated virus (AAV) group received sham 
      operation, the myocardial I/R injury model was prepared by ligation of the left 
      anterior descending coronary artery in I/R group, E2+I/R group, NC siRNA AAV+I/R 
      group, NC siRNA AAV+E2+I/R group and ERbeta-siRNA AAV+E2+I/R group. E2+I/R group, NC 
      siRNA AAV+E2+I/R group and ERbeta-siRNA AAV+E2+I/R group were treated with E2 0.8 
      mg/kg by gavage for 60 days before modeling. NC siRNA AAV+I/R group, NC siRNA 
      AAV+E2+I/R group, and ERbeta-siRNA AAV+E2+I/R group were treated with AAV by caudal 
      vein injection 24 h before modeling. After 120 min of reperfusion, the contents 
      of serum lactate dehydrogenase (LDH), phosphocreatine kinase (CK), 
      phosphocreatine kinase isoenzyme (CK-MB), myocardial infarction area and the 
      expressions of ERbeta, p-ERK, the contents of tumor necrosis factor-alpha(TNF-alpha), 
      interleukin-1beta(IL-1 beta), malondialdehyde (MDA) and total antioxidant capacity 
      (T-AOC) in myocardium were measured. Results: The contents of serum LDH, CK, 
      CK-MB, myocardial infarction area and the contents of TNF-alpha, IL-1 beta, MDA in 
      myocardium of I/R group were higher than those of the control group, the 
      expression levels of ERbeta and p-ERK and the content of T-AOC were lower than those 
      in the control group (P＜0.05). The contents of serum LDH, CK and CK-MB, 
      myocardial infarction area and the contents of TNF-alpha, IL-1 beta and MDA in 
      myocardium of E2+I/R group were lower than those of the I/R group, the expression 
      levels of ERbeta and p-ERK and the content of T-AOC were higher than those of the 
      I/R group(P＜0.05). After knockdown ERbeta by caudal vein injection of ERbeta-siRNA AAV, 
      the contents of serum LDH, CK and CK-MB, myocardial infarction area and the 
      contents of TNF-alpha, IL-1 beta and MDA in myocardium of ERbeta-siRNA AAV+E2+I/R group 
      were higher than those of NC-siRNA AAV+E2+I/R, the expression levels of ERbeta and 
      p-ERK and the content of T-AOC were lower than those of NC-siRNA 
      AAV+E2+I/R(P＜0.05). Conclusion: E2 has protective effects on myocardial I / R 
      injury in ovariectomized rats, which are related to the promotion of ERbeta 
      mediating the activation of ERK pathway, reducing inflammatory and oxidative 
      stress responses.
FAU - Feng, Jing-Ru
AU  - Feng JR
AD  - Department of Gynecology, the Second Affiliated Hospital of Hebei North 
      University, Zhangjiakou 075100.
FAU - Zhang, Hai-Yang
AU  - Zhang HY
AD  - Department of Cardiology, the Second Affiliated Hospital of Hebei North 
      University, Zhangjiakou 075100.
FAU - Shi, He
AU  - Shi H
AD  - Department of Pharmacology, the Fourth Hospital of Shijiazhuang, Shijiazhuang 
      050011.
FAU - Wang, Teng-Fei
AU  - Wang TF
AD  - Clinical Laboratory, the Second Affiliated Hospital of Hebei North University, 
      Zhangjiakou 075100, China.
FAU - Wang, Zi-Jian
AU  - Wang ZJ
AD  - Department of Cardiology, the Second Affiliated Hospital of Hebei North 
      University, Zhangjiakou 075100.
FAU - Cheng, Guang-Hui
AU  - Cheng GH
AD  - Department of Cardiology, the Second Affiliated Hospital of Hebei North 
      University, Zhangjiakou 075100.
FAU - Bi, Sheng-Li
AU  - Bi SL
AD  - Department of Gynecology, the Second Affiliated Hospital of Hebei North 
      University, Zhangjiakou 075100.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 4TI98Z838E (Estradiol)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Estrogen Receptor beta)
RN  - 020IUV4N33 (Phosphocreatine)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.3.2 (Creatine Kinase)
RN  - EC 2.7.3.2 (Creatine Kinase, MB Form)
RN  - 0 (Antioxidants)
SB  - IM
MH  - Female
MH  - Animals
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Estradiol
MH  - *Myocardial Reperfusion Injury
MH  - Interleukin-1beta
MH  - Estrogen Receptor beta
MH  - Phosphocreatine
MH  - Tumor Necrosis Factor-alpha
MH  - RNA, Small Interfering
MH  - *Myocardial Ischemia
MH  - Creatine Kinase
MH  - Creatine Kinase, MB Form
MH  - *Myocardial Infarction
MH  - Antioxidants
OTO - NOTNLM
OT  - estradiol
OT  - estrogen receptor beta
OT  - extracellular regulated protein kinase
OT  - inflammatory response
OT  - myocardial ischemia/reperfusion
OT  - oxidative stress response
OT  - rats
EDAT- 2023/06/13 01:13
MHDA- 2023/06/14 06:42
CRDT- 2023/06/12 22:03
PHST- 2023/06/14 06:42 [medline]
PHST- 2023/06/13 01:13 [pubmed]
PHST- 2023/06/12 22:03 [entrez]
AID - 10.12047/j.cjap.6362.2022.116 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022 Nov;38(6):638-643. doi: 
      10.12047/j.cjap.6362.2022.116.