PMID- 37308428
OWN - NLM
STAT- MEDLINE
DCOM- 20230614
LR  - 20230614
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 38
IP  - 6
DP  - 2022 Nov
TI  - [Effects of fucoidan inducing impairment of human osteosarcoma cell 143B and its 
      mechanism].
PG  - 739-744
LID - 10.12047/j.cjap.6331.2022.135 [doi]
AB  - Objective: To investigate the effects of fucoidan inducing impairment of human 
      osteosarcoma cell 143B, as well its mechanisms. Methods: After 143B cells were 
      treated with different concentrations of FUC (0, 0.5, 1, 10, 100, 400, 800 mug/ml) 
      for 48 h, the cell viability and dehydrogenase (LDH) level were detected by MTT 
      assay and chemical colorimetry with six multiple wells for each concentration. 
      Based on MTT results, we determined the value of IC(50) was 244.5 mug/ml. The 
      follow-up experiments were divided into control group (without FUC), FUC (10 
      mug/ml)-treated group, FUC (100 mug/ml)-treated group, FUC (400 mug/ml)-treated 
      group and positive group (resveratrol, 40 mumol/L). There were four multiple wells 
      for each concentration, and each experiment was repeated at least three times. 
      Flow cytometry was performed to detect cell apoptosis and intracellular reactive 
      oxygen species (ROS) level; acridine orange (AO) staining and lyso-tracker red 
      staining were used to observe the autophagolysosome formation; chemical 
      colorimetric analysis was performed to determine malondialdehyde (MDA) content 
      and the activities of superoxide dismutase (SOD) and glutathione peroxidase 
      (GSH-Px); Western blot was used to detect protein expressions of nuclear factor 
      E2-associated factor 2 (Nrf2), heme oxygenase 1 (HO-1) and autophagy-associated 
      proteins including microtubule-associated light chain protein 3 (LC-3), Atg7, 
      Beclin-1 and p62. Results: Compared with control group, the cell viability was 
      decreased significantly in FUC (100～400 mug/ml)-treated groups (P＜0.01); LDH 
      levels in the supernatant (P＜0.05 or P＜0.01), the percentage of cell apoptosis 
      (P＜0.01), intracellular ROS level and MDA content (P＜0.01) were increased 
      remarkably; protein expressions of Atg7 and Beclin-1 were upregulated (P＜0.05 or 
      P＜0.01); the conversion from LC-3I to LC-3II was significant (P＜0.01) together 
      with elevation of autophagolysosome formation (P＜0.05 or P＜0.01); while the 
      activities of SOD and GSH-Px and protein expressions of Nrf2, HO-1 and p62 were 
      decreased remarkably (P＜0.05 or P＜0.01). Conclusion: FUC (100～400 mug/ml) 
      treatment induces oxidative damage and autophagic death in osteosarcoma 143B 
      cells.
FAU - Wang, Qi-Qi
AU  - Wang QQ
AD  - College of Medicine, Shaoxing University, Shaoxing 312000, China.
FAU - Lin, Qiao
AU  - Lin Q
AD  - College of Medicine, Shaoxing University, Shaoxing 312000, China.
FAU - Shan, Wei-Yan
AU  - Shan WY
AD  - College of Medicine, Shaoxing University, Shaoxing 312000, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - College of Medicine, Shaoxing University, Shaoxing 312000, China.
FAU - Li, Yu-Rong
AU  - Li YR
AD  - College of Medicine, Shaoxing University, Shaoxing 312000, China.
FAU - Zhang, Yun
AU  - Zhang Y
AD  - College of Medicine, Shaoxing University, Shaoxing 312000, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 9072-19-9 (fucoidan)
RN  - 0 (Beclin-1)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
SB  - IM
MH  - Humans
MH  - Beclin-1
MH  - NF-E2-Related Factor 2
MH  - Reactive Oxygen Species
MH  - *Osteosarcoma
MH  - Glutathione Peroxidase
MH  - *Bone Neoplasms
OTO - NOTNLM
OT  - autophagy
OT  - cell culture
OT  - fucoidan
OT  - osteosarcoma 143B cells
OT  - oxidative damage
EDAT- 2023/06/13 01:13
MHDA- 2023/06/14 06:42
CRDT- 2023/06/12 22:03
PHST- 2023/06/14 06:42 [medline]
PHST- 2023/06/13 01:13 [pubmed]
PHST- 2023/06/12 22:03 [entrez]
AID - 10.12047/j.cjap.6331.2022.135 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022 Nov;38(6):739-744. doi: 
      10.12047/j.cjap.6331.2022.135.