PMID- 37309647
OWN - NLM
STAT- MEDLINE
DCOM- 20230623
LR  - 20230625
IS  - 1759-9679 (Electronic)
IS  - 1759-9660 (Linking)
VI  - 15
IP  - 24
DP  - 2023 Jun 22
TI  - Point of care testing (POCT) of cholesterol in blood serum via a moving reaction 
      boundary electrophoresis titration chip.
PG  - 2971-2978
LID - 10.1039/d3ay00550j [doi]
AB  - Cholesterol (CHO) in human blood is one of the most frequently and crucially 
      quantified substances in diagnostic laboratories. However, visual and portable 
      point of care testing (POCT) methods have been rarely developed for the bioassay 
      of CHO in blood samples. Here, we developed an electrophoresis titration (ET) 
      model, a chip device of  approximately 60 grams, and a quantification method for the POCT of 
      CHO in blood serum based on a moving reaction boundary (MRB). In this model, the 
      selective enzymatic reaction is integrated with an ET chip for visual and 
      portable quantification. At first, CHO reacted with cholesterol oxidase (CHOx) in 
      the anode well, producing H(2)O(2) and cholest-4-en-3-one in the solution. 
      H(2)O(2) further oxidized the colorless and chargeless leucocrystal violet (LCV) 
      dye into violet colored positively charged crystal violet (CV(+)) and, under the 
      influence of the electric field, the CV(+) migrates in the ET channels and is 
      titrated by the alkali of sodium hydroxide immobilized in the ET channels. The 
      length covered by the MRB was measured as a function of the CHO content. The 
      relevant experiments validated the feasibility of the model and method. 
      Furthermore, the experiments revealed the high selectivity, portability, and 
      visuality of the ET-MRB model, device, and method. Finally, the experiments 
      showed a fair sensitivity of LOD of 5 muM, good linearity of 10-1000 muM (r(2) = 
      0.9919), fair stability (intra-day RSD of less than 5.09% and an inter-day RSD of 
      less than 6.36%), and high recovery (99.4-105%). All the data and results 
      indicate the potential of the ET-MRB model, chip device, and method for POCT of 
      CHO in human blood samples.
FAU - Khan, Muhammad Idrees
AU  - Khan MI
AD  - School of Sensing Science and Engineering, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. 
      cxcao@sjtu.edu.cn.
FAU - Zhang, Qiang
AU  - Zhang Q
AUID- ORCID: 0000-0001-7603-1530
AD  - School of Sensing Science and Engineering, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. 
      cxcao@sjtu.edu.cn.
FAU - Tian, Youli
AU  - Tian Y
AD  - School of Life Sciences and Biotechnology, State Key Laboratory of Microbial 
      Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Saud, Shah
AU  - Saud S
AD  - School of Sensing Science and Engineering, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. 
      cxcao@sjtu.edu.cn.
FAU - Cao, Yiren
AU  - Cao Y
AD  - School of Sensing Science and Engineering, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. 
      cxcao@sjtu.edu.cn.
AD  - School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, 
      Shanghai 200240, China.
FAU - Ren, Jicun
AU  - Ren J
AUID- ORCID: 0000-0002-8157-5548
AD  - School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, 
      Shanghai 200240, China.
FAU - Liu, Weiwen
AU  - Liu W
AD  - School of Sensing Science and Engineering, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. 
      cxcao@sjtu.edu.cn.
AD  - Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 
      China.
FAU - Cao, Chengxi
AU  - Cao C
AUID- ORCID: 0000-0002-3873-5112
AD  - School of Sensing Science and Engineering, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. 
      cxcao@sjtu.edu.cn.
AD  - School of Life Sciences and Biotechnology, State Key Laboratory of Microbial 
      Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China.
AD  - School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, 
      Shanghai 200240, China.
AD  - Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230622
PL  - England
TA  - Anal Methods
JT  - Analytical methods : advancing methods and applications
JID - 101519733
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.1.3.6 (Cholesterol Oxidase)
SB  - IM
MH  - Humans
MH  - *Serum
MH  - *Hydrogen Peroxide/chemistry
MH  - Electrophoresis/methods
MH  - Cholesterol Oxidase
MH  - Point-of-Care Testing
EDAT- 2023/06/13 06:42
MHDA- 2023/06/23 06:42
CRDT- 2023/06/13 04:42
PHST- 2023/06/23 06:42 [medline]
PHST- 2023/06/13 06:42 [pubmed]
PHST- 2023/06/13 04:42 [entrez]
AID - 10.1039/d3ay00550j [doi]
PST - epublish
SO  - Anal Methods. 2023 Jun 22;15(24):2971-2978. doi: 10.1039/d3ay00550j.