PMID- 37310074
OWN - NLM
STAT- MEDLINE
DCOM- 20231027
LR  - 20231112
IS  - 1607-8438 (Electronic)
IS  - 0300-8207 (Linking)
VI  - 64
IP  - 6
DP  - 2023 Nov
TI  - Knockdown of LncRNA DICER1-AS1 arrests the cell cycle, inhibits cell 
      proliferation, and induces cell apoptosis by regulating CDC5L nuclear transfer in 
      osteosarcoma.
PG  - 519-531
LID - 10.1080/03008207.2023.2223289 [doi]
AB  - BACKGROUND: DICER1-AS1 is reported to promote the progression and disturb the 
      cell cycle in osteosarcoma; however, its mechanism has rarely been studied. 
      MATERIALS AND METHODS: DICER1-AS1 expression levels were evaluated by qPCR and 
      fluorescence in situ hybridization (FISH). The total, nuclear, and cytosolic 
      levels of CDC5L were measured by western blotting and immunofluorescence (IF). 
      Cell proliferation, apoptosis, and cell cycle analyses were conducted using the 
      colony formation, CCK-8 assay, terminal transferase-mediated UTP nick 
      end-labeling kit (TUNEL) assay, and flow cytometry. Levels of cell 
      proliferation-, cell cycle-, and cell apoptosis-related proteins were determined 
      by western blotting. RNA immunoprecipitation (RIP) and RNA pull-down assays were 
      conducted to evaluate the relationship between DICER1-AS1 and CDC5L. RESULTS: 
      LncRNA DICER1-AS1 was highly expressed in samples of osteosarcoma tissue and in 
      osteosarcoma cell lines. DICER1-AS1 knockdown inhibited cell proliferation, 
      promoted cell apoptosis, and disturbed the cell cycle. Moreover, DICER1-AS1 was 
      found to bind with CDC5L, and knockdown of DICER-AS1 inhibited the nuclear 
      transfer of CDC5L. DICER1-AS1 knockdown also reversed the effects of CDC5L 
      overexpression on cell proliferation, apoptosis, and the cell cycle. Moreover, 
      CDC5L inhibition suppressed cell proliferation, promoted cell apoptosis, and 
      disturbed the cell cycle, and those effects were further enhanced by DICER1-AS1 
      knockdown. Finally, DICER1-AS knockdown inhibited tumor growth and proliferation, 
      and promoted cell apoptosis in vivo. CONCLUSION: LncRNA DICER1-AS1 knockdown 
      inhibits the nuclear transfer of CDC5L protein, arrests the cell cycle, and 
      induces apoptosis to suppress the development of osteosarcoma. Our results 
      suggest a novel target (DICER1-AS1) for treatment of osteosarcoma.
FAU - Fu, Laihua
AU  - Fu L
AD  - National Cancer Center, National Clinical Research Center for Cancer, Cancer 
      Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking 
      Union Medical College, Shenzhen, China.
FAU - Xu, Songfeng
AU  - Xu S
AD  - National Cancer Center, National Clinical Research Center for Cancer, Cancer 
      Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking 
      Union Medical College, Shenzhen, China.
FAU - Zhou, Yang
AU  - Zhou Y
AD  - National Cancer Center, National Clinical Research Center for Cancer, Cancer 
      Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking 
      Union Medical College, Shenzhen, China.
FAU - Huang, Jingyang
AU  - Huang J
AD  - National Cancer Center, National Clinical Research Center for Cancer, Cancer 
      Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking 
      Union Medical College, Shenzhen, China.
FAU - Qiu, Jin
AU  - Qiu J
AD  - National Cancer Center, National Clinical Research Center for Cancer, Cancer 
      Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking 
      Union Medical College, Shenzhen, China.
FAU - Huang, Pengzhou
AU  - Huang P
AD  - National Cancer Center, National Clinical Research Center for Cancer, Cancer 
      Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking 
      Union Medical College, Shenzhen, China.
LA  - eng
PT  - Journal Article
DEP - 20230613
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (MicroRNAs)
RN  - EC 3.1.26.3 (DICER1 protein, human)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
RN  - 0 (CDC5L protein, human)
SB  - IM
MH  - Humans
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Cell Proliferation/genetics
MH  - Cell Cycle/genetics
MH  - *Osteosarcoma/genetics
MH  - *Bone Neoplasms/genetics/metabolism/pathology
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - *MicroRNAs/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Ribonuclease III/genetics/metabolism
MH  - DEAD-box RNA Helicases/genetics/metabolism
OTO - NOTNLM
OT  - CDC5L
OT  - DICER1-AS1
OT  - Osteosarcoma
OT  - cell cycle
OT  - nuclear/cytosolic sublocation
EDAT- 2023/06/13 13:12
MHDA- 2023/10/27 06:42
CRDT- 2023/06/13 08:33
PHST- 2023/10/27 06:42 [medline]
PHST- 2023/06/13 13:12 [pubmed]
PHST- 2023/06/13 08:33 [entrez]
AID - 10.1080/03008207.2023.2223289 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2023 Nov;64(6):519-531. doi: 10.1080/03008207.2023.2223289. 
      Epub 2023 Jun 13.