PMID- 37311562
OWN - NLM
STAT- MEDLINE
DCOM- 20230628
LR  - 20230702
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 95
IP  - 25
DP  - 2023 Jun 27
TI  - Real-Time Volatile Metabolomics Analysis of Dendritic Cells.
PG  - 9415-9421
LID - 10.1021/acs.analchem.3c00516 [doi]
AB  - Dendritic cells (DCs) actively sample and present antigen to cells of the 
      adaptive immune system and are thus vital for successful immune control and 
      memory formation. Immune cell metabolism and function are tightly interlinked, 
      and a better understanding of this interaction offers potential to develop 
      immunomodulatory strategies. However, current approaches for assessing the immune 
      cell metabolome are often limited by end-point measurements, may involve 
      laborious sample preparation, and may lack unbiased, temporal resolution of the 
      metabolome. In this study, we present a novel setup coupled to a secondary 
      electrospray ionization-high resolution mass spectrometric (SESI-HRMS) platform 
      allowing headspace analysis of immature and activated DCs in real-time with 
      minimal sample preparation and intervention, with high technical reproducibility 
      and potential for automation. Distinct metabolic signatures of DCs treated with 
      different supernatants (SNs) of bacterial cultures were detected during real-time 
      analyses over 6 h compared to their respective controls (SN only). Furthermore, 
      the technique allowed for the detection of (13)C-incorporation into volatile 
      metabolites, opening the possibility for real-time tracing of metabolic pathways 
      in DCs. Moreover, differences in the metabolic profile of naïve and activated 
      DCs were discovered, and pathway-enrichment analysis revealed three significantly 
      altered pathways, including the TCA cycle, alpha-linolenic acid metabolism, and 
      valine, leucine, and isoleucine degradation.
FAU - Arnold, Kim
AU  - Arnold K
AD  - University Children's Hospital Basel (UKBB), 4056 Basel, Switzerland.
AD  - Department of Biomedical Engineering, University of Basel, 4123 Allschwil, 
      Switzerland.
FAU - Dehio, Philippe
AU  - Dehio P
AD  - Department of Biomedicine, Immunobiology, University of Basel and University 
      Hospital of Basel, 4031 Basel, Switzerland.
FAU - Lotscher, Jonas
AU  - Lotscher J
AD  - Department of Biomedicine, Immunobiology, University of Basel and University 
      Hospital of Basel, 4031 Basel, Switzerland.
FAU - Singh, Kapil Dev
AU  - Singh KD
AUID- ORCID: 0000-0002-5682-0708
AD  - University Children's Hospital Basel (UKBB), 4056 Basel, Switzerland.
AD  - Department of Biomedical Engineering, University of Basel, 4123 Allschwil, 
      Switzerland.
FAU - Garcia-Gomez, Diego
AU  - Garcia-Gomez D
AUID- ORCID: 0000-0001-5753-2962
AD  - Department of Analytical Chemistry, Nutrition and Food Science, University of 
      Salamanca, 37008 Salamanca, Spain.
FAU - Hess, Christoph
AU  - Hess C
AD  - Department of Biomedicine, Immunobiology, University of Basel and University 
      Hospital of Basel, 4031 Basel, Switzerland.
AD  - Department of Medicine, CITIID, Jeffrey Cheah Biomedical Centre, University of 
      Cambridge, Cambridge CB2 0AW, United Kingdom.
FAU - Sinues, Pablo
AU  - Sinues P
AUID- ORCID: 0000-0001-5602-2880
AD  - University Children's Hospital Basel (UKBB), 4056 Basel, Switzerland.
AD  - Department of Biomedical Engineering, University of Basel, 4123 Allschwil, 
      Switzerland.
FAU - Balmer, Maria L
AU  - Balmer ML
AUID- ORCID: 0000-0003-0356-6352
AD  - Department of Biomedicine, Immunobiology, University of Basel and University 
      Hospital of Basel, 4031 Basel, Switzerland.
AD  - Department of Biomedical Research (DBMR), University of Bern, 3008 Bern, 
      Switzerland.
AD  - University Clinic for Diabetes, Endocrinology, Clinical Nutrition and Metabolism, 
      Inselspital, 3010 Bern, Switzerland.
AD  - Diabetes Center Bern (DCB), 3010 Bern, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230613
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
SB  - IM
MH  - *Spectrometry, Mass, Electrospray Ionization/methods
MH  - Reproducibility of Results
MH  - *Metabolomics/methods
MH  - Metabolome
MH  - Dendritic Cells
PMC - PMC10308329
COIS- The authors declare the following competing financial interest(s): P.S. is 
      cofounder of Deep Breath Intelligence A.G. (Switzerland), which develops 
      breath-based diagnostic tools. K.D.S. is a consultant for Deep Breath 
      Intelligence A.G. (Switzerland).
EDAT- 2023/06/14 01:10
MHDA- 2023/06/28 06:42
PMCR- 2023/06/29
CRDT- 2023/06/13 19:42
PHST- 2023/06/28 06:42 [medline]
PHST- 2023/06/14 01:10 [pubmed]
PHST- 2023/06/13 19:42 [entrez]
PHST- 2023/06/29 00:00 [pmc-release]
AID - 10.1021/acs.analchem.3c00516 [doi]
PST - ppublish
SO  - Anal Chem. 2023 Jun 27;95(25):9415-9421. doi: 10.1021/acs.analchem.3c00516. Epub 
      2023 Jun 13.