PMID- 37313021
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 2008-126X (Print)
IS  - 2228-7442 (Electronic)
IS  - 2008-126X (Linking)
VI  - 13
IP  - 5
DP  - 2022 Sep-Oct
TI  - Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in 
      Dopaminergic Neurons in a Three-Dimensional Culture.
PG  - 625-636
LID - 10.32598/bcn.2021.973.3 [doi]
AB  - INTRODUCTION: The induction of human umbilical cord-derived mesenchymal stem 
      cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue 
      engineering and experimental and clinical treatments of various neurodegenerative 
      diseases, including Parkinson disease. This study aims to differentiate HUC-MSCs 
      into dopaminergic neuron-like cells. METHODS: Following the isolation and 
      characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and 
      incubated with a cocktail of dopaminergic neuronal differentiation factors. The 
      capacity of differentiation into dopaminergic neuron-like cells in 2-dimensional 
      culture and on Matrigel was assessed by real-time polymerase chain reaction, 
      immunocytochemistry, and high-performance liquid chromatography. RESULTS: Our 
      results showed that dopaminergic neuronal markers' transcript and protein levels 
      were significantly increased on the Matrigel differentiated cells compared to 2D 
      culture plates. CONCLUSION: Overall, the results of this study suggest that 
      HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on 
      Matrigel, having great potential for the treatment of dopaminergic neuron-related 
      diseases.
CI  - Copyright(c) 2022 Iranian Neuroscience Society.
FAU - Moayeri, Ardeshir
AU  - Moayeri A
AUID- ORCID: 0000-0002-3704-5347
AD  - Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, 
      Ilam, Iran.
FAU - Alizadeh, Rafieh
AU  - Alizadeh R
AUID- ORCID: 0000-0002-3132-9904
AD  - ENT and Head and Neck Research Center and Department, The Five Senses Health 
      Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
FAU - Ghasemi Hamidabadi, Hatef
AU  - Ghasemi Hamidabadi H
AUID- ORCID: 0000-0002-2178-6503
AD  - Department of Anatomy & Cell Biology, Immunogenetic Research Center, School of 
      Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
FAU - Nazm Bojnordi, Maryam
AU  - Nazm Bojnordi M
AUID- ORCID: 0000-0003-1176-2417
AD  - Department of Anatomy & Cell Biology, Immunogenetic Research Center, School of 
      Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
FAU - Niapour, Ali
AU  - Niapour A
AUID- ORCID: 0000-0002-9013-4664
AD  - Department of Anatomical Sciences, Research Laboratory for Embryology and Stem 
      Cells, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
FAU - Hedayatpour, Azim
AU  - Hedayatpour A
AUID- ORCID: 0000-0002-1036-0697
AD  - Department of Anatomy, School of Medicine, University of Tehran, Tehran, Iran.
FAU - Darvishi, Marzieh
AU  - Darvishi M
AUID- ORCID: 0000-0002-5004-6213
AD  - Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, 
      Ilam, Iran.
FAU - Heidari, Fatemeh
AU  - Heidari F
AUID- ORCID: 0000-0003-3822-3480
AD  - Department of Anatomy, School of Medicine, Qom University of Medical Sciences, 
      Qom, Iran.
FAU - Soleimani, Maryam
AU  - Soleimani M
AUID- ORCID: 0000-0003-2650-9174
AD  - Department of Basic Sciences, School of Rehabilitation Sciences, University of 
      Social Welfare and Rehabilitation Sciences, Tehran, Iran.
FAU - Elyasi, Leila
AU  - Elyasi L
AUID- ORCID: 0000-0002-2618-2434
AD  - Department of Anatomy, Neuroscience Research Center, School of Medicine, Golestan 
      University of Medical Sciences, Gorgan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220901
PL  - Iran
TA  - Basic Clin Neurosci
JT  - Basic and clinical neuroscience
JID - 101575211
PMC - PMC10258594
OTO - NOTNLM
OT  - Differentiation
OT  - Dopaminergic neurons
OT  - Human umbilical cord mesenchymal stem cells
OT  - In vitro
COIS- Conflict of interest The authors declared no conflict of interest.
EDAT- 2023/06/14 06:42
MHDA- 2023/06/14 06:43
PMCR- 2022/09/01
CRDT- 2023/06/14 03:51
PHST- 2020/07/27 00:00 [received]
PHST- 2021/03/03 00:00 [revised]
PHST- 2021/08/07 00:00 [accepted]
PHST- 2023/06/14 06:43 [medline]
PHST- 2023/06/14 06:42 [pubmed]
PHST- 2023/06/14 03:51 [entrez]
PHST- 2022/09/01 00:00 [pmc-release]
AID - BCN-13-625 [pii]
AID - 10.32598/bcn.2021.973.3 [doi]
PST - ppublish
SO  - Basic Clin Neurosci. 2022 Sep-Oct;13(5):625-636. doi: 10.32598/bcn.2021.973.3. 
      Epub 2022 Sep 1.