PMID- 37315291
OWN - NLM
STAT- MEDLINE
DCOM- 20230627
LR  - 20230701
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 15
IP  - 11
DP  - 2023 Jun 13
TI  - LAMP2A, and other chaperone-mediated autophagy related proteins, do not decline 
      with age in genetically heterogeneous UM-HET3 mice.
PG  - 4685-4698
LID - 10.18632/aging.204796 [doi]
AB  - Chaperone-mediated autophagy (CMA) selectively degrades proteins that are crucial 
      for glycolysis, fatty acid metabolism, and the progression of several 
      age-associated diseases. Several previous studies, each of which evaluated males 
      of a single inbred mouse or rat strain, have reported that CMA declines with age 
      in many tissues, attributed to an age-related loss of LAMP2A, the primary and 
      indispensable component of the CMA translocation complex. This has led to a 
      paradigm in the field of CMA research, stating that the age-associated decline in 
      LAMP2A in turn decreases CMA, contributing to the pathogenesis of late-life 
      disease. We assessed LAMP2A levels and CMA substrate uptake in both sexes of the 
      genetically heterogeneous UM-HET3 mouse stock, which is the current global 
      standard for the evaluation of anti-aging interventions. We found no evidence for 
      age-related changes in LAMP2A levels, CMA substrate uptake, or whole liver levels 
      of CMA degradation targets, despite identifying sex differences in CMA.
FAU - Zhang, Katherine K
AU  - Zhang KK
AD  - University of Michigan, College of Literature, Science, and The Arts, Ann Arbor, 
      MI 48109, USA.
FAU - Zhang, Peichuan
AU  - Zhang P
AD  - Calico Life Sciences, South San Francisco, CA 94080, USA.
AD  - Current Affiliation: WuXi AppTec, Shanghai, China.
FAU - Kodur, Anagha
AU  - Kodur A
AD  - University of Michigan, College of Literature, Science, and The Arts, Ann Arbor, 
      MI 48109, USA.
FAU - Erturk, Ilkim
AU  - Erturk I
AD  - University of Michigan, Department of Pathology, Ann Arbor, MI 48109, USA.
FAU - Burns, Calvin M
AU  - Burns CM
AD  - University of Michigan, Department of Pathology, Ann Arbor, MI 48109, USA.
FAU - Kenyon, Cynthia
AU  - Kenyon C
AD  - Calico Life Sciences, South San Francisco, CA 94080, USA.
FAU - Miller, Richard A
AU  - Miller RA
AD  - University of Michigan, Department of Pathology, Ann Arbor, MI 48109, USA.
AD  - University of Michigan Geriatrics Center, Ann Arbor, MI 48109, USA.
FAU - Endicott, S Joseph
AU  - Endicott SJ
AD  - University of Michigan, Department of Pathology, Ann Arbor, MI 48109, USA.
AD  - University of Michigan Geriatrics Center, Ann Arbor, MI 48109, USA.
LA  - eng
GR  - P30 AG024824/AG/NIA NIH HHS/United States
GR  - U19 AG023122/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20230613
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Autophagy-Related Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Lysosomal-Associated Membrane Protein 2)
SB  - IM
MH  - Animals
MH  - Female
MH  - Male
MH  - Mice
MH  - Rats
MH  - Aging/genetics
MH  - Autophagy/genetics
MH  - Autophagy-Related Proteins/metabolism
MH  - *Chaperone-Mediated Autophagy/genetics
MH  - Lysosomes/metabolism
MH  - Molecular Chaperones/genetics/metabolism
MH  - Lysosomal-Associated Membrane Protein 2/metabolism
PMC - PMC10292871
OTO - NOTNLM
OT  - aging
OT  - autophagy
OT  - chaperone-mediated autophagy
COIS- CONFLICTS OF INTEREST: The authors have no conflicts of interest to disclose.
EDAT- 2023/06/14 19:10
MHDA- 2023/06/22 06:42
PMCR- 2023/06/15
CRDT- 2023/06/14 16:13
PHST- 2023/04/21 00:00 [received]
PHST- 2023/05/30 00:00 [accepted]
PHST- 2023/06/22 06:42 [medline]
PHST- 2023/06/14 19:10 [pubmed]
PHST- 2023/06/14 16:13 [entrez]
PHST- 2023/06/15 00:00 [pmc-release]
AID - 204796 [pii]
AID - 10.18632/aging.204796 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2023 Jun 13;15(11):4685-4698. doi: 10.18632/aging.204796. Epub 
      2023 Jun 13.