PMID- 37316758
OWN - NLM
STAT- MEDLINE
DCOM- 20230811
LR  - 20230811
IS  - 1559-1182 (Electronic)
IS  - 0893-7648 (Linking)
VI  - 60
IP  - 9
DP  - 2023 Sep
TI  - The Temporal and Spatial Changes of Autophagy and PI3K Isoforms in Different 
      Neural Cells After Hypoxia/Reoxygenation Injury.
PG  - 5366-5377
LID - 10.1007/s12035-023-03421-9 [doi]
AB  - There are limited therapeutic options for patient with traumatic spinal cord 
      injury (SCI). Phosphoinositide 3-kinase family (PI3Ks) are the key molecules for 
      regulating cell autophagy, which is a possible way of treating SCI. As we know, 
      PI3K family are composed of eight isoforms, which are distributed into three 
      classes. While the role of PI3Ks in regulating autophagy is controversial and the 
      effects may be in a cell-specific manner. Different isoforms do not distribute in 
      neural cells consistently and it is not clear how the PI3K isoforms regulate and 
      interact with autophagy. Therefore, we explored the distributions and expression 
      of different PI3K isoforms in two key neural cells (PC12 cells and astrocytes). 
      The results showed that the expression of LC3II/I and p62, which are the markers 
      of autophagy, changed in different patterns in PC12 cells and astrocytes after 
      hypoxia/reoxygenation injury (H/R). Furthermore, the mRNA level of eight PI3K 
      isoforms did not change in the same way, and even for the same isoform the mRNA 
      activities are different between PC12 cells and astrocytes. What is more, the 
      results of western blot of PI3K isoforms after H/R were inconsistent with the 
      relevant mRNA. Based on this study, the therapeutic effects of regulating 
      autophagy on SCI are not confirmed definitely, and its molecular mechanisms may 
      be related with different temporal and spatial patterns of activation and 
      distributions of PI3K isoforms.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Zhang, Duo
AU  - Zhang D
AD  - Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing, 100070, China.
FAU - Chen, Xuanyu
AU  - Chen X
AD  - Department of Orthopedics, Capital Medical University Electric Power Hospital, 
      Beijing, 100073, China.
FAU - Liu, Baoge
AU  - Liu B
AUID- ORCID: 0000-0002-2156-0870
AD  - Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing, 100070, China. baogeliu@hotmail.com.
FAU - Yuan, Yuan
AU  - Yuan Y
AD  - Department of Spinal Cord Injury Rehabilitation, China Rehabilitation Research 
      Center, Beijing, 100068, China.
FAU - Cui, Wei
AU  - Cui W
AD  - Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing, 100070, China.
FAU - Zhu, Di
AU  - Zhu D
AD  - Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing, 100070, China.
FAU - Zhu, Jichao
AU  - Zhu J
AD  - Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing, 100070, China.
FAU - Duan, Shuo
AU  - Duan S
AD  - Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing, 100070, China.
FAU - Li, Chenxi
AU  - Li C
AD  - Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing, 100070, China.
LA  - eng
GR  - 2017000021469G215/Beijing Excellent Talent Training Funding/
GR  - PYZ2017082/the Natural Science Foundation of Capital Medical University of China/
GR  - PYZ2018081/the Natural Science Foundation of Capital Medical University of China/
GR  - 2018YFF0301103/the National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20230614
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Humans
MH  - *Phosphatidylinositol 3-Kinases
MH  - *Autophagy
MH  - Hypoxia
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - RNA, Messenger
MH  - Apoptosis
OTO - NOTNLM
OT  - Astrocytes
OT  - Autophagy
OT  - Hypoxia/reoxygenation injury
OT  - PC12 cells
OT  - PI3K isoforms
OT  - Spinal cord injury
EDAT- 2023/06/15 01:08
MHDA- 2023/08/11 06:43
CRDT- 2023/06/14 23:30
PHST- 2022/07/27 00:00 [received]
PHST- 2023/06/02 00:00 [accepted]
PHST- 2023/08/11 06:43 [medline]
PHST- 2023/06/15 01:08 [pubmed]
PHST- 2023/06/14 23:30 [entrez]
AID - 10.1007/s12035-023-03421-9 [pii]
AID - 10.1007/s12035-023-03421-9 [doi]
PST - ppublish
SO  - Mol Neurobiol. 2023 Sep;60(9):5366-5377. doi: 10.1007/s12035-023-03421-9. Epub 
      2023 Jun 14.