PMID- 37317084
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230617
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 11
IP  - 5
DP  - 2023 Apr 24
TI  - Polyhydroxybutyrate Production from Methane and Carbon Dioxide by a Syntrophic 
      Consortium of Methanotrophs with Oxygenic Photogranules without an External 
      Oxygen Supply.
LID - 10.3390/microorganisms11051110 [doi]
LID - 1110
AB  - Here, a syntrophic process was developed to produce polyhydroxy-beta-butyrate (PHB) 
      from a gas stream containing CH(4) and CO(2) without an external oxygen supply 
      using a combination of methanotrophs with the community of oxygenic photogranules 
      (OPGs). The co-culture features of Methylomonas sp. DH-1 and Methylosinus 
      trichosporium OB3b were evaluated under carbon-rich and carbon-lean conditions. 
      The critical role of O(2) in the syntrophy was confirmed through the sequencing 
      of 16S rRNA gene fragments. Based on their carbon consumption rates and the 
      adaptation to a poor environment, M. trichosporium OB3b with OPGs was selected 
      for methane conversion and PHB production. Nitrogen limitation stimulated PHB 
      accumulation in the methanotroph but hindered the growth of the syntrophic 
      consortium. At 2.9 mM of the nitrogen source, 1.13 g/L of biomass and 83.0 mg/L 
      of PHB could be obtained from simulated biogas. These results demonstrate that 
      syntrophy has the potential to convert greenhouse gases into valuable products 
      efficiently.
FAU - Ashoor, Selim
AU  - Ashoor S
AUID- ORCID: 0000-0003-1552-6657
AD  - Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams 
      University, Hadayek Shoubra, Cairo 11241, Egypt.
AD  - Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 
      04107, Republic of Korea.
FAU - Jun, Seong-Hoon
AU  - Jun SH
AD  - Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 
      04107, Republic of Korea.
FAU - Ko, Han Do
AU  - Ko HD
AD  - Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 
      04107, Republic of Korea.
FAU - Lee, Jinwon
AU  - Lee J
AD  - Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 
      04107, Republic of Korea.
FAU - Hamelin, Jerome
AU  - Hamelin J
AUID- ORCID: 0000-0003-3601-6349
AD  - INRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, 
      France.
FAU - Milferstedt, Kim
AU  - Milferstedt K
AUID- ORCID: 0000-0002-1186-3982
AD  - INRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, 
      France.
FAU - Na, Jeong-Geol
AU  - Na JG
AUID- ORCID: 0000-0003-2196-7547
AD  - Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 
      04107, Republic of Korea.
LA  - eng
GR  - NRF-2015M3D3A1A01064926/National Research Foundation of Korea/
GR  - NRF-2019K1A3A1A21031925/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20230424
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10224382
OTO - NOTNLM
OT  - greenhouse gas mitigation
OT  - methanotrophy
OT  - oxygenic photogranules
OT  - polyhydroxybutyrate
OT  - syntrophic process
COIS- The authors declare no conflict of interest.
EDAT- 2023/06/15 06:42
MHDA- 2023/06/15 06:43
PMCR- 2023/04/24
CRDT- 2023/06/15 01:01
PHST- 2023/03/26 00:00 [received]
PHST- 2023/04/16 00:00 [revised]
PHST- 2023/04/22 00:00 [accepted]
PHST- 2023/06/15 06:43 [medline]
PHST- 2023/06/15 06:42 [pubmed]
PHST- 2023/06/15 01:01 [entrez]
PHST- 2023/04/24 00:00 [pmc-release]
AID - microorganisms11051110 [pii]
AID - microorganisms-11-01110 [pii]
AID - 10.3390/microorganisms11051110 [doi]
PST - epublish
SO  - Microorganisms. 2023 Apr 24;11(5):1110. doi: 10.3390/microorganisms11051110.