PMID- 3731817
OWN - NLM
STAT- MEDLINE
DCOM- 19860916
LR  - 20190912
IS  - 0011-2240 (Print)
IS  - 0011-2240 (Linking)
VI  - 23
IP  - 3
DP  - 1986 Jun
TI  - Glycerol stress and platelet integrity.
PG  - 209-13
AB  - Platelet response to glycerol gradient was studied using a few in vitro 
      parameters. These were platelet count, mean platelet size, platelet response to 
      hypotonic stress (PHRS), and collagen-induced platelet aggregation. An equal 
      volume of 1-10% (w/v) glycerol in plasma was added at once to the platelet 
      concentrate resulting in 0.5-5% (w/v) final glycerol concentration. The 
      concentrate was kept at 22 degrees C for 60 min. Platelets were then separated by 
      one centrifugation and resuspended in glycerol-free plasma. A loss in platelet 
      count was observed when the gradient of glycerol was more than 3%. This was 
      associated with an increase in mean cell size and a reduction in aggregability. 
      With 5% glycerol stress, a loss of 30% in cell count, an increase in 18% in cell 
      size, and a 78% loss in aggregability was observed. Declining of PRHS was shown 
      already with a 1% glycerol gradient and 69% of this function was suppressed by 5% 
      glycerol stress. In other experiments, 5% glycerol was first added, them removed 
      in 5 steps with a gradient of 1% each. When time interval between each step was 
      less than 0.5 min, platelet loss and PRHS reduction were 17 and 47% respectively. 
      These values were gradually improved to 4% and 11-20%, respectively, as 
      increasing time interval up to 15 min. It was concluded that a gradient of 1% 
      glycerol and a 15-min interval for each step minimizes the detrimental osmotic 
      stress on platelets while glycerol is added or removed. Our findings may lead up 
      to devising an improved protocol for platelet cryopreservation with glycerol.
FAU - Kim, B K
AU  - Kim BK
FAU - Baldini, M G
AU  - Baldini MG
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cryobiology
JT  - Cryobiology
JID - 0006252
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - Blood Platelets/*cytology/drug effects
MH  - Freezing
MH  - Glycerol/*pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Stress, Mechanical
MH  - Tissue Preservation/methods
EDAT- 1986/06/01 00:00
MHDA- 1986/06/01 00:01
CRDT- 1986/06/01 00:00
PHST- 1986/06/01 00:00 [pubmed]
PHST- 1986/06/01 00:01 [medline]
PHST- 1986/06/01 00:00 [entrez]
AID - 0011-2240(86)90046-5 [pii]
AID - 10.1016/0011-2240(86)90046-5 [doi]
PST - ppublish
SO  - Cryobiology. 1986 Jun;23(3):209-13. doi: 10.1016/0011-2240(86)90046-5.