PMID- 37335485
OWN - NLM
STAT- MEDLINE
DCOM- 20230621
LR  - 20230622
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2672
DP  - 2023
TI  - Chromosome Painting Using Chromosome-Specific BAC Clones.
PG  - 303-313
LID - 10.1007/978-1-0716-3226-0_19 [doi]
AB  - Chromosome painting (CP) refers to visualization of large chromosome regions, 
      chromosome arms or entire chromosomes via fluorescence in situ hybridization 
      (FISH) of chromosome-specific DNA sequences. For CP in crucifers (Brassicaceae), 
      typically contigs of chromosome-specific bacterial artificial chromosomes (BAC) 
      from Arabidopsis thaliana are applied as painting probes on chromosomes of A. 
      thaliana or other species (comparative chromosome painting, CCP). CP/CCP enables 
      to identify and trace particular chromosome regions and/or chromosomes throughout 
      all mitotic and meiotic stages as well as corresponding interphase chromosome 
      territories. However, extended pachytene chromosomes provide the highest 
      resolution of CP/CCP. Fine-scale chromosome structure, structural chromosome 
      rearrangements (such as inversions, translocations, centromere repositioning), 
      and chromosome breakpoints can be investigated by CP/CCP. BAC DNA probes can be 
      accompanied by other types of DNA probes, such as repetitive DNA, genomic DNA, or 
      synthetic oligonucleotide probes. Here, we describe a robust step-by-step 
      protocol of CP and CCP which proved to be efficient across the family 
      Brassicaceae, but which is also applicable to other angiosperm families.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Mandakova, Terezie M
AU  - Mandakova TM
AD  - CEITEC - Central European Institute of Technology, Masaryk University, Brno, 
      Czech Republic. terezie.mandakova@ceitec.muni.cz.
FAU - Lysak, Martin A
AU  - Lysak MA
AD  - CEITEC - Central European Institute of Technology, Masaryk University, Brno, 
      Czech Republic. martin.lysak@ceitec.muni.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 9007-49-2 (DNA)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosome Painting/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes
MH  - *Brassicaceae/genetics
MH  - *Arabidopsis/genetics
MH  - DNA
MH  - DNA Probes
MH  - Clone Cells
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - BAC FISH
OT  - Brassicaceae
OT  - Chromosome painting
OT  - Fluorescence in situ hybridization (FISH)
OT  - Nick translation
EDAT- 2023/06/19 13:08
MHDA- 2023/06/21 06:42
CRDT- 2023/06/19 11:10
PHST- 2023/06/21 06:42 [medline]
PHST- 2023/06/19 13:08 [pubmed]
PHST- 2023/06/19 11:10 [entrez]
AID - 10.1007/978-1-0716-3226-0_19 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2672:303-313. doi: 10.1007/978-1-0716-3226-0_19.