PMID- 37335487
OWN - NLM
STAT- MEDLINE
DCOM- 20230621
LR  - 20230622
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2672
DP  - 2023
TI  - Preparation of Male Meiotic Chromosomes for Fluorescence In Situ Hybridization 
      and Immunodetection with Major Focus on Dogroses.
PG  - 337-349
LID - 10.1007/978-1-0716-3226-0_21 [doi]
AB  - Meiosis is a very essential cell division resulting in the formation of four 
      haploid gametes in plants. The preparation of meiotic chromosomes is a key step 
      in plant meiotic research. Well-spread chromosomes, low background signal, and 
      effective cell wall elimination give the best hybridization results. Dogroses 
      (Rosa, section Caninae) are allopolyploids and frequently pentaploids 
      (2n = 5x = 35) with asymmetrical meiosis. Their cytoplasm is enriched with 
      organic compounds such as vitamins, tannins, phenols, essential oils, and many 
      more. The cytoplasm is often a huge problem, avoiding successful cytogenetic 
      experiments using fluorescence staining techniques. Here, we present a protocol 
      with modifications for the preparation of male meiotic chromosomes suitable for 
      fluorescence in situ hybridization (FISH) and immunolabeling with a major focus 
      on dogroses.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Lunerova, Jana
AU  - Lunerova J
AD  - Department of Epigenetics, Institute of Biophysics of the Czech Academy of 
      Sciences, Kralovopolska, Brno, Czech Republic. jana.luner@ibp.cz.
FAU - Vozarova, Radka
AU  - Vozarova R
AD  - Department of Epigenetics, Institute of Biophysics of the Czech Academy of 
      Sciences, Kralovopolska, Brno, Czech Republic.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Polyploidy
MH  - *Chromosomes
MH  - Germ Cells
MH  - Meiosis/genetics
OTO - NOTNLM
OT  - Asymmetrical meiosis
OT  - Chromosome preparation
OT  - FISH
OT  - Flower buds
OT  - Immunodetection
OT  - Meiosis
OT  - Rosa
EDAT- 2023/06/19 13:08
MHDA- 2023/06/21 06:42
CRDT- 2023/06/19 11:10
PHST- 2023/06/21 06:42 [medline]
PHST- 2023/06/19 13:08 [pubmed]
PHST- 2023/06/19 11:10 [entrez]
AID - 10.1007/978-1-0716-3226-0_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2672:337-349. doi: 10.1007/978-1-0716-3226-0_21.