PMID- 37337400
OWN - NLM
STAT- MEDLINE
DCOM- 20240221
LR  - 20240221
IS  - 1600-0781 (Electronic)
IS  - 0905-4383 (Linking)
VI  - 39
IP  - 6
DP  - 2023 Nov
TI  - A decrease of mitochondrial ubiquitin ligase increases the secretion of matrix 
      metalloproteinase-1 by dermal fibroblasts through the induction of ER stress.
PG  - 582-588
LID - 10.1111/phpp.12897 [doi]
AB  - BACKGROUND: We previously reported that the level of mitochondrial ubiquitin 
      ligase (MITOL) protein in fibroblasts was decreased by UVA and that the 
      knock-down (KD) of MITOL increased the secretion of matrix metalloprotease-1 
      (MMP-1) by fibroblasts. A recent study reported that MITOL suppresses endoplasmic 
      reticulum (ER) stress by stabilizing the interaction between ER and mitochondria 
      (MT) through the ubiquitination of mitofusin 2. These facts suggest that a 
      decrease of MITOL would increase the secretion of MMP-1 through ER stress, but 
      the detailed mechanism of that process in dermal fibroblasts remains unclear. 
      Thus, this study was conducted to clarify the involvement of ER stress in the 
      oversecretion of MMP-1 induced by the decreased MT quality caused by MITOL-KD. 
      METHODS: MITOL-KD normal human dermal fibroblast (NHDFs) were prepared by 
      treating them with MITOL-small interfering RNA, after which their MMP-1 protein 
      levels were measured. ER stress in NHDFs was evaluated by measuring the mRNA 
      levels of spliced X-box binding protein 1 (sXBP1) and the protein levels of 
      inositol-requiring enzyme 1alpha (IRE1alpha). RESULTS: MITOL-KD NHDFs enhanced the 
      secretion of MMP-1 via interleukin-6 (IL-6) elicited by the activation of nuclear 
      factor-kappa B (NF-kappaB). The secretion of MMP-1 could be abrogated by a 
      neutralizing IL-6 antibody and by JSH23, which is an inhibitor of NF-kappaB 
      activation. Furthermore, MITOL-KD NHDFs as well as UVA-irradiated NHDFs showed 
      increased ER stress levels. In addition, tunicamycin, which is an inducer of ER 
      stress, also increased MMP-1 secretion. CONCLUSION: These results suggested that 
      the decrease of MITOL caused the oversecretion of MMP-1 via NF-kappaB-IL-6 signaling 
      through the activation of ER stress in fibroblasts.
CI  - (c) 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Katsuyama, Yushi
AU  - Katsuyama Y
AUID- ORCID: 0000-0002-7608-3370
AD  - CIEL CO., LTD, Sagamihara, Japan.
FAU - Okano, Yuri
AU  - Okano Y
AD  - CIEL CO., LTD, Sagamihara, Japan.
FAU - Masaki, Hitoshi
AU  - Masaki H
AD  - CIEL CO., LTD, Sagamihara, Japan.
AD  - Research Institute for Human Health Science, Konan University, Kobe, Japan.
LA  - eng
PT  - Journal Article
DEP - 20230619
PL  - England
TA  - Photodermatol Photoimmunol Photomed
JT  - Photodermatology, photoimmunology & photomedicine
JID - 9013641
RN  - EC 3.1.- (Endoribonucleases)
RN  - 0 (Interleukin-6)
RN  - EC 6.- (Ligases)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 0 (NF-kappa B)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.27 (MARCHF5 protein, human)
RN  - EC 3.4.24.7 (MMP1 protein, human)
SB  - IM
MH  - Humans
MH  - Endoribonucleases/metabolism
MH  - Fibroblasts/metabolism
MH  - Interleukin-6
MH  - Ligases/metabolism
MH  - *Matrix Metalloproteinase 1/genetics
MH  - Matrix Metalloproteinase 3/metabolism
MH  - *NF-kappa B/metabolism
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Ubiquitins/metabolism
OTO - NOTNLM
OT  - endoplasmic reticulum stress
OT  - matrix metalloproteinase-1
OT  - mitochondria
OT  - mitochondrial ubiquitin ligase
EDAT- 2023/06/20 06:42
MHDA- 2024/02/12 18:42
CRDT- 2023/06/20 01:02
PHST- 2023/06/01 00:00 [revised]
PHST- 2023/02/25 00:00 [received]
PHST- 2023/06/07 00:00 [accepted]
PHST- 2024/02/12 18:42 [medline]
PHST- 2023/06/20 06:42 [pubmed]
PHST- 2023/06/20 01:02 [entrez]
AID - 10.1111/phpp.12897 [doi]
PST - ppublish
SO  - Photodermatol Photoimmunol Photomed. 2023 Nov;39(6):582-588. doi: 
      10.1111/phpp.12897. Epub 2023 Jun 19.