PMID- 37338083
OWN - NLM
STAT- Publisher
LR  - 20230620
IS  - 1601-0825 (Electronic)
IS  - 1354-523X (Linking)
DP  - 2023 Jun 20
TI  - Effect and mechanism of fluctuant glucose on restraining implant osseointegration 
      in diabetes.
LID - 10.1111/odi.14600 [doi]
AB  - OBJECTIVES: The objectives of the study was to discuss the effect and mechanism 
      of fluctuant glucose (FG) on implant osseointegration in type 2 diabetic mellitus 
      (T2DM). MATERIALS AND METHODS: Rats were divided into control, T2DM and FG group, 
      and the implants were inserted into their femurs. Micro-CT and histological 
      analysis were used to evaluate the effect on osseointegration in vivo. And we 
      investigated the effect of different conditions (normal, control, high glucose, 
      and FG medium) on rat osteoblast in vitro. Then transmission electron microscope 
      (TEM) and Western blot were used to evaluate the endoplasmic reticulum stress 
      (ERS) response. Finally, 4-PBA, an inhibitor of ERS, was added into different 
      conditions to observe the functions of osteoblast. RESULTS: In vivo, Micro-CT and 
      histological analysis showed that the percentage of osseointegration in FG rats 
      were lower than other two group. In vitro, the results demonstrated that the 
      adhesion of the cells becomes worst, and osteogenic ability was also severely 
      impaired in FG group. In addition, FG could induce more serious ERS and 4-PBA 
      could improve the dysfunction of osteoblasts induced by FG. CONCLUSION: Fluctuant 
      glucose could restrain the implant osseointegration in T2DM, and the effect was 
      more obvious than consistent high glucose by a possible mechanism of activation 
      ERS pathway.
CI  - (c) 2023 Wiley Periodicals LLC.
FAU - Song, Dawei
AU  - Song D
AD  - Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong 
      First Medical University, Jinan, China.
AD  - School of Stomatology, Shandong First Medical University and Shandong Academy of 
      Medical Sciences, Jinan, China.
FAU - Wang, Chenchen
AU  - Wang C
AD  - Department of Oral Medicine, Qilu Hospital of Shandong University, Jinan, China.
FAU - Liang, Jin
AU  - Liang J
AD  - Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong 
      First Medical University, Jinan, China.
AD  - School of Stomatology, Shandong First Medical University and Shandong Academy of 
      Medical Sciences, Jinan, China.
FAU - Jia, Tingting
AU  - Jia T
AUID- ORCID: 0000-0001-6696-5436
AD  - Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong 
      First Medical University, Jinan, China.
AD  - School of Stomatology, Shandong First Medical University and Shandong Academy of 
      Medical Sciences, Jinan, China.
LA  - eng
GR  - 82071148/National Natural Science Foundation of China/
GR  - ZR2022QH283/Natural Science Foundation of Shandong Province/
GR  - 2021FY016/Research Incubation Fund of Shandong Provincial Hospital/
PT  - Journal Article
DEP - 20230620
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
OTO - NOTNLM
OT  - endoplasmic reticulum stress
OT  - fluctuant glucose
OT  - osseointegration
OT  - type 2 diabetic mellitus
EDAT- 2023/06/20 13:10
MHDA- 2023/06/20 13:10
CRDT- 2023/06/20 07:43
PHST- 2023/04/04 00:00 [revised]
PHST- 2022/08/18 00:00 [received]
PHST- 2023/04/14 00:00 [accepted]
PHST- 2023/06/20 13:10 [medline]
PHST- 2023/06/20 13:10 [pubmed]
PHST- 2023/06/20 07:43 [entrez]
AID - 10.1111/odi.14600 [doi]
PST - aheadofprint
SO  - Oral Dis. 2023 Jun 20. doi: 10.1111/odi.14600.