PMID- 37338646
OWN - NLM
STAT- Publisher
LR  - 20231021
IS  - 1615-6102 (Electronic)
IS  - 0033-183X (Linking)
VI  - 260
IP  - 6
DP  - 2023 Nov
TI  - Downregulation of ITGA5 inhibits lymphangiogenesis and cell migration and 
      invasion in male laryngeal squamous cell carcinoma.
PG  - 1569-1580
LID - 10.1007/s00709-023-01873-3 [doi]
AB  - ITGA5, a fibronectin receptor was highly expressed in laryngeal squamous cell 
      carcinoma (LSCC) samples and was related to poor survival. However, the potential 
      mechanism remains unclear. To elucidate the regulatory role of ITGA5 in LSCC 
      progression, we investigated the effect of ITGA5 expression on lymphangiogenesis, 
      migration, and invasion of LSCC cells in vitro and in vivo using 
      immunohistochemistry, siRNA transfection, qRT-PCR, western blotting, 
      enzyme-linked immunosorbent assay, flow cytometry, transwell co-culture, tube 
      formation, cell migration, and invasion assays, and a subcutaneous graft tumor 
      model. The expression of ITGA5 was higher in the LSCC tissues and linked to lymph 
      node metastasis and T staging. Moreover, ITGA5 expression was significantly 
      positively correlated with VEGF-C expression, and the lymphatic vessel density of 
      patients with high ITGA5 expression was noticeably higher than that of patients 
      with low ITGA5 expression. Additionally, it was found in vitro that 
      downregulation of ITGA5 expression not only inhibited the expression and 
      secretion of VEGF-C, but also suppressed the tube-forming ability of human 
      lymphatic endothelial cells (HLECs) and the migration and invasion ability of 
      LSCC cells, while exogenous VEGF-C supplementation reversed these phenomena. 
      Furthermore, a tumor xenograft assay showed that si-ITGA5 restrained the growth 
      and metastasis of TU212-derived tumors in vivo. Our findings suggested that ITGA5 
      induces lymphangiogenesis and LSCC cell migration and invasion by enhancing 
      VEGF-C expression and secretion.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Wang, Xiaoting
AU  - Wang X
AD  - Central Laboratory, Fujian Medical University Union Hospital, 29 Xinquan Road, 
      Fuzhou, 350001, Fujian, China.
FAU - Huang, Jun
AU  - Huang J
AD  - Central Laboratory, Fujian Medical University Union Hospital, 29 Xinquan Road, 
      Fuzhou, 350001, Fujian, China.
AD  - Department of Clinical Laboratory, Fujian Medical University Union Hospital, 
      Fuzhou, China.
FAU - You, Ruolan
AU  - You R
AD  - Central Laboratory, Fujian Medical University Union Hospital, 29 Xinquan Road, 
      Fuzhou, 350001, Fujian, China.
FAU - Hou, Diyu
AU  - Hou D
AD  - Central Laboratory, Fujian Medical University Union Hospital, 29 Xinquan Road, 
      Fuzhou, 350001, Fujian, China.
FAU - Liu, Jingru
AU  - Liu J
AD  - Central Laboratory, Fujian Medical University Union Hospital, 29 Xinquan Road, 
      Fuzhou, 350001, Fujian, China.
FAU - Wu, Long
AU  - Wu L
AD  - Department of Pathology, Fujian Medical University Union Hospital, Fuzhou, China.
FAU - Yao, Meihong
AU  - Yao M
AD  - Department of Pathology, Fujian Medical University Union Hospital, Fuzhou, China.
FAU - Yang, Fuwen
AU  - Yang F
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The 900th Hospital of 
      the People's Liberation Army Joint Service Support Force, 156 North Xi-er Huan 
      Road, Fuzhou, 350025, Fujian, China. puwenyang@163.com.
FAU - Huang, Huifang
AU  - Huang H
AUID- ORCID: 0000-0002-8548-028X
AD  - Central Laboratory, Fujian Medical University Union Hospital, 29 Xinquan Road, 
      Fuzhou, 350001, Fujian, China. huanghuif@fjmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230620
PL  - Austria
TA  - Protoplasma
JT  - Protoplasma
JID - 9806853
SB  - IM
OTO - NOTNLM
OT  - ITGA5
OT  - Laryngeal squamous cell carcinoma
OT  - Lymphangiogenesis
OT  - Metastasis
OT  - VEGF-C
EDAT- 2023/06/20 13:10
MHDA- 2023/06/20 13:10
CRDT- 2023/06/20 11:09
PHST- 2023/02/28 00:00 [received]
PHST- 2023/06/09 00:00 [accepted]
PHST- 2023/06/20 13:10 [pubmed]
PHST- 2023/06/20 13:10 [medline]
PHST- 2023/06/20 11:09 [entrez]
AID - 10.1007/s00709-023-01873-3 [pii]
AID - 10.1007/s00709-023-01873-3 [doi]
PST - ppublish
SO  - Protoplasma. 2023 Nov;260(6):1569-1580. doi: 10.1007/s00709-023-01873-3. Epub 
      2023 Jun 20.