PMID- 37340281
OWN - NLM
STAT- MEDLINE
DCOM- 20230622
LR  - 20230622
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Linking)
VI  - 136
IP  - 7
DP  - 2023 Jun 20
TI  - Genome sequence analysis provides insights into the mode of 2n egg formation in 
      Solanum malmeanum.
PG  - 157
LID - 10.1007/s00122-023-04406-x [doi]
AB  - Our genomic investigation confirms the mechanism of 2n eggs formation in S. 
      malmeanum and aid in optimizing the use of wild germplasm. Wild potatoes are a 
      valuable source of agronomic traits. However, substantial reproductive barriers 
      limit gene flow into cultivated species. 2n gametes are instrumental in 
      preventing endosperm abortion caused by genetic imbalances in the endosperm. 
      However, little is known about the molecular mechanisms underlying the formation 
      of 2n gametes. Here, the wild species Solanum malmeanum Bitter (2x, 1EBN, 
      endosperm balance number) was used in inter- and intrapoloid crosses with other 
      Solanum species, with viable seeds being produced only when S. malmeanum was used 
      as the female parent to cross the 2EBN Solanum genus and with the likely 
      involvement of 2n gametes. Subsequently, we substantiated the formation of 2n 
      eggs in S. malmeanum using fluorescence in situ hybridization (FISH) and genomic 
      sequencing technology. Additionally, the transmission rate of maternal 
      heterozygous polymorphism sites was assessed from a genomic perspective to 
      analyze the mode of 2n egg formation in S. malmeanum x S. tuberosum and S. 
      malmeanum x S. chacoense crosses; each cross acquired an average of 31.12% and 
      22.79% maternal sites, respectively. This confirmed that 2n egg formation in S. 
      malmeanum attributed to second-division restitution (SDR) coupled with the 
      occurrence of exchange events. The high-throughput sequencing technology used in 
      this study has strong advantages over traditional cytological analyses. 
      Furthermore, S. malmeanum, which has a variety of excellent traits not available 
      from present cultivated potato genepool, has received little research attention 
      and has successfully achieved gene flow in cultivated species in the current 
      study. These findings will facilitate the understanding and optimization of wild 
      germplasm utilization in potatoes.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Dong, Jianke
AU  - Dong J
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Tu, Wei
AU  - Tu W
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
AD  - College of Biology and Agricultural Resources, Huanggang Normal University, 
      Huanggang, 438000, China.
FAU - Wang, Haibo
AU  - Wang H
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
AD  - College of Biological and Food Engineering, Hubei Minzu University, Enshi, 
      445000, China.
FAU - Zuo, Yingtao
AU  - Zuo Y
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Liu, Tengfei
AU  - Liu T
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Zhao, Qinghao
AU  - Zhao Q
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Ying, Jingwen
AU  - Ying J
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Wu, Jianghai
AU  - Wu J
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Liu, Yuanyuan
AU  - Liu Y
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Cai, Xingkui
AU  - Cai X
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Song, Botao
AU  - Song B
AUID- ORCID: 0000-0002-8493-1181
AD  - National Key Laboratory for Germplasm Innovation and Utilization of Horticultural 
      Crops, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key 
      Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural 
      Affairs, Huazhong Agricultural University, Wuhan, 430070, China. 
      songbotao@mail.hzau.edu.cn.
LA  - eng
GR  - 2022B0202060001/Key-Area Research and Development Program of Guangdong Province/
GR  - CARS-09-P07/Key Laboratory of South China Sea Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture and Rural Affairs, P. R. China/
GR  - 202211404/Project of High-level Teachers in Beijing Municipal Universities in the 
      Period of 13th Five-year Plan/
PT  - Journal Article
DEP - 20230620
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
SB  - IM
MH  - *Solanum/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Solanum tuberosum/genetics
MH  - Heterozygote
MH  - Seeds/genetics
EDAT- 2023/06/21 01:07
MHDA- 2023/06/22 06:42
CRDT- 2023/06/20 23:33
PHST- 2023/04/10 00:00 [received]
PHST- 2023/06/13 00:00 [accepted]
PHST- 2023/06/22 06:42 [medline]
PHST- 2023/06/21 01:07 [pubmed]
PHST- 2023/06/20 23:33 [entrez]
AID - 10.1007/s00122-023-04406-x [pii]
AID - 10.1007/s00122-023-04406-x [doi]
PST - epublish
SO  - Theor Appl Genet. 2023 Jun 20;136(7):157. doi: 10.1007/s00122-023-04406-x.