PMID- 37340915
OWN - NLM
STAT- MEDLINE
DCOM- 20230623
LR  - 20230623
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 39
IP  - 6
DP  - 2023 Jun
TI  - [miR-877-3p causes osteoporosis in mice by inhibiting MCP-1 secretion from mouse 
      bone marrow mesenchymal stem cells and the migration and apoptosis of T 
      lymphocytes].
PG  - 481-487
AB  - Objective To investigate the effects of miR-877-3p on migration and apoptotic T 
      lymphocytes of bone mesenchymal stem cells (BMSCs). Methods The model of 
      osteoporosis induced by bilateral ovariectomy (OVX) and sham operation was 
      established. At 8 weeks after operation, the bone parameters of the two groups 
      were detected by micro-CT. The levels of monocyte chemotactic protein 1(MCP-1) in 
      BMSCs were detected by ELISA. BMSC in OVX group and sham group were co-cultured 
      with T lymphocytes, respectively. The migration ability of T lymphocytes in the 
      two groups was observed by Transwell(TM) assay with PKH26 staining and apoptosis 
      of T lymphocytes were detected by flow cytometry. Reverse transcription PCR was 
      used to detect the expression of miR-877-3p in BMSCs. miR-877-3p was 
      overexpressed or down-regulated by cell transfection. The level of MCP-1 secreted 
      by BMSCs in each group was detected by ELISA. The migration and apoptosis of T 
      lymphocytes were detected by the above methods. Results The number of trabecular 
      bone and bone mineral density in OVX group were lower than those in sham group. 
      The levels of MCP-1 secretion, chemotactic and apoptotic T lymphocyte ability of 
      BMSCs in OVX group were also lower than those in sham group. The expression level 
      of miR-877-3p in BMSC in OVX group was higher than that in sham group. After 
      overexpression of BMSC miR-877-3p, the levels of MCP-1 secreted from BMSCs, and 
      apoptotic T lymphocytes decreased, while the results were opposite after 
      down-regulation of miR-877-3p. Conclusion miR-877-3p may be one of the causes of 
      osteoporosis by inhibiting MCP-1 secretion of BMSCs and the migration and 
      apoptosis of T lymphocytes.
FAU - Qin, Jie
AU  - Qin J
AD  - Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory 
      of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of 
      Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory 
      of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of 
      Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. 
      *Corresponding author, E-mail: 500439@hospital.cqmu.edu.cn.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Chemokine CCL2)
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN877 microRNA, mouse)
SB  - IM
MH  - Animals
MH  - Female
MH  - Mice
MH  - Apoptosis/genetics
MH  - Bone Marrow Cells/metabolism
MH  - Cell Differentiation
MH  - Chemokine CCL2/genetics/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Osteogenesis
MH  - *Osteoporosis/genetics
MH  - T-Lymphocytes/metabolism
EDAT- 2023/06/21 06:42
MHDA- 2023/06/22 06:42
CRDT- 2023/06/21 04:23
PHST- 2023/06/22 06:42 [medline]
PHST- 2023/06/21 06:42 [pubmed]
PHST- 2023/06/21 04:23 [entrez]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2023 Jun;39(6):481-487.