PMID- 37343839 OWN - NLM STAT- MEDLINE DCOM- 20230808 LR - 20230808 IS - 1872-8359 (Electronic) IS - 0167-7012 (Linking) VI - 211 DP - 2023 Aug TI - Factors affecting soil treatment with the microbially induced carbonate precipitation technique and its optimization. PG - 106771 LID - S0167-7012(23)00105-7 [pii] LID - 10.1016/j.mimet.2023.106771 [doi] AB - The microbially induced carbonate precipitation (MICP) technique has been used to increase mechanical strength, reduce permeability, and fix radionuclides of soils, etc. To achieve effective soil cementation by MICP, 3 aspects should be considered: MICP efficiency, bacterium retention (in soils after injections), and precipitation uniformity. Here, experiments and statistical analyses were conducted to understand the parameters affecting the 3 aspects. Moreover, the parameters leading to better performance in these aspects were designed and used to conduct MICP soil cementation with varying the number of injections. The results present that temperature and OD600nm of bacterial suspension are the most important parameters affecting MICP efficiency, followed by reaction time, pH, and concentration of cementation solution, and they are all statistically significant. As these parameters increased, MICP efficiency (ratio of CaCO(3) formed to Ca(2+) added) first increased quickly and then slowly or decreased. The soil particle size distribution and injection rate affected bacterium retention greatly. Smaller particle sizes, wider particle-size-distribution spans, and slower injection rates are beneficial to bacterium retention. However, higher injection rates favour precipitation uniformity. Finally, the unconfined compressive strength (UCS) of the bio-treated soil can be increased further by increasing the number of injections. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - Su, Fei AU - Su F AD - State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, China; China Institute of Nuclear Industry Strategy, Beijing 100045, China. FAU - Wang, Yajian AU - Wang Y AD - State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, China; National Center for Materials Service Safety, University of Science and Technology Beijing (USTB), Beijing 100083, China; School of Engineering and Technology, China University of Geosciences (Beijing), Beijing 100083, China. Electronic address: wangyj@ustb.edu.cn. FAU - Liu, Yunqi AU - Liu Y AD - China Institute of Nuclear Industry Strategy, Beijing 100045, China. FAU - Zhang, Jiaqi AU - Zhang J AD - China Institute of Nuclear Industry Strategy, Beijing 100045, China. FAU - Liu, Xing AU - Liu X AD - China Institute of Nuclear Industry Strategy, Beijing 100045, China. FAU - Zhang, Siyuan AU - Zhang S AD - China Institute of Nuclear Industry Strategy, Beijing 100045, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230619 PL - Netherlands TA - J Microbiol Methods JT - Journal of microbiological methods JID - 8306883 RN - 0 (Soil) RN - H0G9379FGK (Calcium Carbonate) RN - 0 (Carbonates) SB - IM MH - *Soil MH - *Calcium Carbonate MH - Chemical Precipitation MH - Carbonates MH - Bacteria OTO - NOTNLM OT - Bacterium retention OT - Injection rate OT - MICP efficiency OT - MICP soil cementation OT - Particle size distribution OT - Precipitation uniformity COIS- Declaration of Competing Interest No conflict of interest was declared. EDAT- 2023/06/22 01:07 MHDA- 2023/08/08 06:42 CRDT- 2023/06/21 19:17 PHST- 2022/11/15 00:00 [received] PHST- 2023/06/13 00:00 [revised] PHST- 2023/06/17 00:00 [accepted] PHST- 2023/08/08 06:42 [medline] PHST- 2023/06/22 01:07 [pubmed] PHST- 2023/06/21 19:17 [entrez] AID - S0167-7012(23)00105-7 [pii] AID - 10.1016/j.mimet.2023.106771 [doi] PST - ppublish SO - J Microbiol Methods. 2023 Aug;211:106771. doi: 10.1016/j.mimet.2023.106771. Epub 2023 Jun 19.