PMID- 37344084 OWN - NLM STAT- MEDLINE DCOM- 20230623 LR - 20230625 IS - 2405-4577 (Electronic) IS - 2405-4577 (Linking) VI - 56 DP - 2023 Aug TI - Eryngium billardieri extract affects cardiac gene expression of master regulators of cardiomyaopathy in rats with high fatdiet-induced insulin resistance. PG - 59-66 LID - S2405-4577(23)00109-2 [pii] LID - 10.1016/j.clnesp.2023.04.016 [doi] AB - BACKGROUND: For years, numerous studies have focused on identifying approaches to increase insulin sensitivity by modifying the signaling factors. In the present study, we examined the effects of Eryngium billardieri extract, as an anti-diabetic herbal medication, on the heart mRNA level of Akt serine/threonine kinase (Akt), mechanistic target of rapamycin kinase (mTOR), peroxisome proliferator-activated receptor gamma (PPARgamma), and Forkhead box o1 (Foxo1) in rats with high-fat diet (HFD)-induced insulin resistance (IR). We also assessed the anti-diabetic effects of E. billardieri extract in rats with insulin resistance. METHODS: Twenty-seven male Wistar rats were divided into two groups. Nine rats were fed a normal diet (control group), and 18 rats were fed an HFD for 13 weeks (HFD group). To confirm the induction of insulin resistance, the oral glucose tolerance test (OGTT) was performed and homeostatic model assessment for insulin resistance (HOMA-IR) was calculated. Then rats with IR were randomly divided into the following groups: the HFD group, which continued an HFD, and the group treated with E. billardieri extract, which received the extract at a concentration of 50 mg/kg for 30 days. On the 30th day, the animals were sacrificed and serum samples were collected for biochemistry analyses. Furthermore, the expression of Akt, mTOR, PPARgamma, and Foxo1 was measured in heart tissue using the real-time polymerase chain reaction (PCR) method. RESULTS: Real-time PCR analyses revealed that an HFD can significantly decrease the expression level of Akt, mTOR, and PPARgamma in the heart tissue. However, an HFD significantly increased the expression level of Foxo1 in the HFD group compared to the control group (P < 0.05). In addition, our data showed that the administration of E. billardieri extract significantly enhanced the mRNA levels of Akt, PPARgamma, and mTOR in the heart tissue compared to the HFD group (P < 0.05), while it significantly decreased the Foxo1 mRNA levels (P < 0.01). CONCLUSION: Given that Akt, mTOR, PPARgamma, and Foxo1 are critical factors in insulin resistance, the present study suggests that E. billardieri could probably be used as an alternative treatment for IR as a major feature of metabolic syndrome. CI - Copyright (c) 2023 European Society for Clinical Nutrition and Metabolism. Published by Elsevier Ltd. All rights reserved. FAU - Osqueei, Mohaddeseh Rashedi AU - Osqueei MR AD - Department of Biochemistry, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran. FAU - Mahmoudabadi, Ali Zaree AU - Mahmoudabadi AZ AD - Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran; Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. FAU - Bahari, Zahra AU - Bahari Z AD - Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Iran. FAU - Meftahi, Gholam Hossein AU - Meftahi GH AD - Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. FAU - Movahedi, Monireh AU - Movahedi M AD - Department of Biochemistry, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran. FAU - Taghipour, Reza AU - Taghipour R AD - Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. FAU - Mousavi, Naser AU - Mousavi N AD - Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. FAU - Huseini, Hasan Fallah AU - Huseini HF AD - Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran. FAU - Jangravi, Zohreh AU - Jangravi Z AD - Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran; Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: jangraviz89@gmail.com. LA - eng PT - Journal Article DEP - 20230508 PL - England TA - Clin Nutr ESPEN JT - Clinical nutrition ESPEN JID - 101654592 RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - 0 (PPAR gamma) RN - 0 (RNA, Messenger) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) SB - IM MH - Rats MH - Male MH - Animals MH - *Insulin Resistance MH - *Eryngium/metabolism MH - Proto-Oncogene Proteins c-akt/genetics/metabolism MH - PPAR gamma/genetics MH - Rats, Wistar MH - RNA, Messenger MH - TOR Serine-Threonine Kinases/genetics MH - Gene Expression OTO - NOTNLM OT - Akt OT - Eryngium billardieri OT - Foxo1 OT - High-fat diet OT - Insulin resistance OT - PPARgamma OT - mTOR COIS- Declaration of competing interest The authors declare that there are no conflicts of interest regarding the publication of this paper. EDAT- 2023/06/22 01:07 MHDA- 2023/06/23 06:42 CRDT- 2023/06/21 20:57 PHST- 2022/06/29 00:00 [received] PHST- 2023/04/05 00:00 [revised] PHST- 2023/04/21 00:00 [accepted] PHST- 2023/06/23 06:42 [medline] PHST- 2023/06/22 01:07 [pubmed] PHST- 2023/06/21 20:57 [entrez] AID - S2405-4577(23)00109-2 [pii] AID - 10.1016/j.clnesp.2023.04.016 [doi] PST - ppublish SO - Clin Nutr ESPEN. 2023 Aug;56:59-66. doi: 10.1016/j.clnesp.2023.04.016. Epub 2023 May 8.